PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS03835 [old locus tag: SACOL0747 ]
• pan locus tag^?: SAUPAN002571000
• symbol: SACOL_RS03835
• pan gene symbol^?: —
• synonym:
• product: GTP-binding protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS03835 [old locus tag: SACOL0747 ]
• symbol: SACOL_RS03835
• product: GTP-binding protein
• replicon: chromosome
• strand: +
• coordinates: 768539..769465
• length: 927
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (768539..769465) NCBI
• BioCyc: SACOL_RS03835 BioCyc
• MicrobesOnline: see SACOL0747

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  841
  901
  
  ATGAAAAATAATAAAGATGAAAAAATAAGAATATCCATAATTAACGGATTTTTGGGTAGT
  GGTAAAACCACGTTACTGACACATTATATTAGTGAATTATTAAAAAATGATGAGAAAATT
  AAAATCATCATGAATGAATTCGGTACTTTTGATATTGATAGCAATAGTATTTCAAATGAA
  ATTGAAGTCCATTCATTGATTAATGGTTGTGTTTGTTGCGATCTTAAACAAGAACTTGTC
  TATGAACTAAAAGCCATTGCTTTAAAAGGGGACGTTAATCATGTCATCATAGAAGCGACA
  GGCATTGCGCATCCTTTGGAATTACTAGTTGCATGTCAAGATCCGCAAATCGTTAATTTC
  TTTGAAAAGCCGATTATTTATGGTGTATTAGATGCGACTCGATTTTTAGAACGTCATCAA
  TATACCGAAAATACAGTTTCGCTGATGGAAGATCAGTTGAAACTAAGTGACATGATTATT
  ATTAATAAAATTGATCTTATAACTGATGACAGTCTTGAGAAAATTGATAAGCAATTAGGT
  ATGATTTGTGCAAGTATTCCAACTTATAAAACAACCTATGGAAAAGTTTCGTTGGAAGAA
  TTGGACTTAACTGTTAAAGACAGAGAGATATCGTCTCATCATCACCATCATCATGGGATT
  AAAAGTATGACTTACACGTTTACAGGTCCGATTGATCGTCATTTGTTTTATCAATTTATA
  ATGAAATTACCGGAATCTGTTCTACGTTTGAAAGGTTATGTGTCATTTAGAGATCAACCA
  AATGCAATTTATGAATTTCAATATGCATATGGTTTACCAGACTATGGAATAATTGGCATG
  CAATTACCATTAACGATTGTTATTATTGGTGAAACTTTAGATACAAATCACATACGTAAT
  CAATTGGATATGCTACAATTTACGTAA
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  927
  

⊟Protein

⊟General

• locus tag: SACOL_RS03835 [old locus tag: SACOL0747 ]
• symbol: SACOL_RS03835
• description: GTP-binding protein
• length: 308
• theoretical pI: 5.52628
• theoretical MW: 35224.4
• GRAVY: -0.0918831

⊟Function

• TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin cobalamin biosynthesis protein CobW (TIGR02475; HMM-score: 101.4)
  and 3 more
  Genetic information processing Protein fate Protein modification and repair hydrogenase accessory protein HypB (TIGR00073; HMM-score: 22.6)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Molybdopterin molybdopterin-guanine dinucleotide biosynthesis protein B (TIGR00176; HMM-score: 15.6)
  KaiC domain protein, AF_0351 family (TIGR03880; HMM-score: 13.6)
• TheSEED: see SACOL0747
• PFAM:
  P-loop_NTPase (CL0023) cobW; CobW/HypB/UreG, nucleotide-binding domain (PF02492; HMM-score: 152.1)
  and 11 more
  no clan defined CobW_C; Cobalamin synthesis protein cobW C-terminal domain (PF07683; HMM-score: 52.1)
  P-loop_NTPase (CL0023) MobB; Molybdopterin guanine dinucleotide synthesis protein B (PF03205; HMM-score: 20.5)
  RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 16.7)
  ATPase_2; ATPase domain predominantly from Archaea (PF01637; HMM-score: 16.4)
  AAA_14; AAA domain (PF13173; HMM-score: 16.3)
  no clan defined DUF2824; Protein of unknown function (DUF2824) (PF11039; HMM-score: 14.9)
  P-loop_NTPase (CL0023) AAA_22; AAA domain (PF13401; HMM-score: 14.3)
  NACHT; NACHT domain (PF05729; HMM-score: 13.5)
  Arf; ADP-ribosylation factor family (PF00025; HMM-score: 12)
  T2SSE; Type II/IV secretion system protein (PF00437; HMM-score: 12)
  AAA_30; AAA domain (PF13604; HMM-score: 11.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.009718
  • TAT(Tat/SPI): 0.000283
  • LIPO(Sec/SPII): 0.001616
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446717094 NCBI
• RefSeq: WP_000794407 NCBI
• UniProt: see SACOL0747

⊟Protein sequence

• MKNNKDEKIRISIINGFLGSGKTTLLTHYISELLKNDEKIKIIMNEFGTFDIDSNSISNEIEVHSLINGCVCCDLKQELVYELKAIALKGDVNHVIIEATGIAHPLELLVACQDPQIVNFFEKPIIYGVLDATRFLERHQYTENTVSLMEDQLKLSDMIIINKIDLITDDSLEKIDKQLGMICASIPTYKTTYGKVSLEELDLTVKDREISSHHHHHHGIKSMTYTFTGPIDRHLFYQFIMKLPESVLRLKGYVSFRDQPNAIYEFQYAYGLPDYGIIGMQLPLTIVIIGETLDTNHIRNQLDMLQFT

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: see SACOL0747
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications