PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA0642 [new locus tag: SA_RS03670 ]
• pan locus tag^?: SAUPAN002571000
• symbol: SA0642
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0642 [new locus tag: SA_RS03670 ]
• symbol: SA0642
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 736087..737013
• length: 927
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123449 NCBI
• RefSeq: NP_373897 NCBI
• BioCyc: see SA_RS03670
• MicrobesOnline: 102923 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
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  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  
  ATGAAAAATAATAAAGATGAAAAAATAAGAATATCCATAATTAACGGATTTTTGGGTAGT
  GGTAAAACCACGTTACTGACACATTATATTAGTGAATTATTAAAAAATGATGAGAAAATT
  AAAATCATCATGAATGAATTCGGTACTTTTGATATTGATAGCAATAGTATTTCAAATGAA
  ATTGAAGTCCATTCATTGATTAATGGTTGTGTTTGTTGCGATCTTAAACAAGAACTTGTC
  TATGAACTAAAAGCCATTGCTTTAAAAGGGGACGTTAATCATGTCATCATAGAAGCGACA
  GGCATTGCGCATCCTTTGGAATTACTAGTTGCATGTCAAGATCCGCAAATCGTTAATTTC
  TTTGAAAAGCCGATTATTTATGGTGTATTAGATGCGACTCGATTTTTAGAACGTCATCAA
  TATACCGAAAATACAGTTTCGCTGATGGAAGAACAGTTGAAACTAAGTGACATGATTATT
  ATTAATAAAATTGATCTTATAACTGATGACAGTCTTGAGAAAATTGATAAGCAATTAGGT
  ATGATTTGTGCAAGTATTCCAACTTATAAAACAACCTATGGAAAAGTTTCGTTGGAAGAA
  TTGGACTTAACTGTTAAAGACAGAGAGATATCGTCTCATCATCACCATCATCATGGGATT
  AAAAGTATGACTTACACGTTTACAGGTCCGATTGATCGTCAATTGTTTTATCAATTTATA
  ATGAAATTACCGGAATCTGTTCTACGTTTGAAAGGTTATGTGTCATTTAGAAATCAACCA
  AATGCAATTTATGAATTTCAATATGCATATGGTTTACCAGACTATGGAATAATTGGCATG
  CAATTACCATTAACGATTGTTATTATTGGTGAAACTTTAGATACAAATCACATACGTAAT
  CAATTGGATATGCTACAATTTACGTAA
  60
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  927
  

⊟Protein

⊟General

• locus tag: SA0642 [new locus tag: SA_RS03670 ]
• symbol: SA0642
• description: hypothetical protein
• length: 308
• theoretical pI: 5.54289
• theoretical MW: 35228.5
• GRAVY: -0.0928571

⊟Function

• TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin cobalamin biosynthesis protein CobW (TIGR02475; HMM-score: 99.6)
  and 3 more
  Genetic information processing Protein fate Protein modification and repair hydrogenase accessory protein HypB (TIGR00073; HMM-score: 23.3)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Molybdopterin molybdopterin-guanine dinucleotide biosynthesis protein B (TIGR00176; HMM-score: 15.6)
  KaiC domain protein, AF_0351 family (TIGR03880; HMM-score: 14.2)
• TheSEED  :
  • Metal chaperone, involved in Zn homeostasis
  COG0523-Histidine  Putative metal chaperone, involved in Zn homeostasis, GTPase of COG0523 family
• PFAM:
  P-loop_NTPase (CL0023) cobW; CobW/HypB/UreG, nucleotide-binding domain (PF02492; HMM-score: 151.4)
  and 13 more
  no clan defined CobW_C; Cobalamin synthesis protein cobW C-terminal domain (PF07683; HMM-score: 52.4)
  P-loop_NTPase (CL0023) MobB; Molybdopterin guanine dinucleotide synthesis protein B (PF03205; HMM-score: 20.5)
  RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 17.5)
  ATPase_2; ATPase domain predominantly from Archaea (PF01637; HMM-score: 16.3)
  AAA_14; AAA domain (PF13173; HMM-score: 16.2)
  no clan defined DUF2824; Protein of unknown function (DUF2824) (PF11039; HMM-score: 14.9)
  P-loop_NTPase (CL0023) AAA_22; AAA domain (PF13401; HMM-score: 14.2)
  NACHT; NACHT domain (PF05729; HMM-score: 13.6)
  ATP_bind_1; Conserved hypothetical ATP binding protein (PF03029; HMM-score: 12.8)
  Arf; ADP-ribosylation factor family (PF00025; HMM-score: 12.5)
  ABC_tran; ABC transporter (PF00005; HMM-score: 12.3)
  T2SSE; Type II/IV secretion system protein (PF00437; HMM-score: 12.1)
  AAA_30; AAA domain (PF13604; HMM-score: 11.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.009718
  • TAT(Tat/SPI): 0.000283
  • LIPO(Sec/SPII): 0.001616
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15926364 NCBI
• RefSeq: NP_373897 NCBI
• UniProt: A0A0H3JK64 UniProt

⊟Protein sequence

• MKNNKDEKIRISIINGFLGSGKTTLLTHYISELLKNDEKIKIIMNEFGTFDIDSNSISNEIEVHSLINGCVCCDLKQELVYELKAIALKGDVNHVIIEATGIAHPLELLVACQDPQIVNFFEKPIIYGVLDATRFLERHQYTENTVSLMEEQLKLSDMIIINKIDLITDDSLEKIDKQLGMICASIPTYKTTYGKVSLEELDLTVKDREISSHHHHHHGIKSMTYTFTGPIDRQLFYQFIMKLPESVLRLKGYVSFRNQPNAIYEFQYAYGLPDYGIIGMQLPLTIVIIGETLDTNHIRNQLDMLQFT

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications