From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS03670 [old locus tag: SA0642 ]
  • pan locus tag?: SAUPAN002571000
  • symbol: SA_RS03670
  • pan gene symbol?:
  • synonym:
  • product: GTP-binding protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS03670 [old locus tag: SA0642 ]
  • symbol: SA_RS03670
  • product: GTP-binding protein
  • replicon: chromosome
  • strand: +
  • coordinates: 736087..737013
  • length: 927
  • essential: no DEG other strains

Accession numbers[edit | edit source]

  • Location: NC_002745 (736087..737013) NCBI
  • BioCyc: SA_RS03670 BioCyc
  • MicrobesOnline: see SA0642

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAAAAATAATAAAGATGAAAAAATAAGAATATCCATAATTAACGGATTTTTGGGTAGT
    GGTAAAACCACGTTACTGACACATTATATTAGTGAATTATTAAAAAATGATGAGAAAATT
    AAAATCATCATGAATGAATTCGGTACTTTTGATATTGATAGCAATAGTATTTCAAATGAA
    ATTGAAGTCCATTCATTGATTAATGGTTGTGTTTGTTGCGATCTTAAACAAGAACTTGTC
    TATGAACTAAAAGCCATTGCTTTAAAAGGGGACGTTAATCATGTCATCATAGAAGCGACA
    GGCATTGCGCATCCTTTGGAATTACTAGTTGCATGTCAAGATCCGCAAATCGTTAATTTC
    TTTGAAAAGCCGATTATTTATGGTGTATTAGATGCGACTCGATTTTTAGAACGTCATCAA
    TATACCGAAAATACAGTTTCGCTGATGGAAGAACAGTTGAAACTAAGTGACATGATTATT
    ATTAATAAAATTGATCTTATAACTGATGACAGTCTTGAGAAAATTGATAAGCAATTAGGT
    ATGATTTGTGCAAGTATTCCAACTTATAAAACAACCTATGGAAAAGTTTCGTTGGAAGAA
    TTGGACTTAACTGTTAAAGACAGAGAGATATCGTCTCATCATCACCATCATCATGGGATT
    AAAAGTATGACTTACACGTTTACAGGTCCGATTGATCGTCAATTGTTTTATCAATTTATA
    ATGAAATTACCGGAATCTGTTCTACGTTTGAAAGGTTATGTGTCATTTAGAAATCAACCA
    AATGCAATTTATGAATTTCAATATGCATATGGTTTACCAGACTATGGAATAATTGGCATG
    CAATTACCATTAACGATTGTTATTATTGGTGAAACTTTAGATACAAATCACATACGTAAT
    CAATTGGATATGCTACAATTTACGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    927

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA_RS03670 [old locus tag: SA0642 ]
  • symbol: SA_RS03670
  • description: GTP-binding protein
  • length: 308
  • theoretical pI: 5.54289
  • theoretical MW: 35228.5
  • GRAVY: -0.0928571

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin cobalamin biosynthesis protein CobW (TIGR02475; HMM-score: 99.6)
    and 3 more
    Genetic information processing Protein fate Protein modification and repair hydrogenase accessory protein HypB (TIGR00073; HMM-score: 23.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Molybdopterin molybdopterin-guanine dinucleotide biosynthesis protein B (TIGR00176; HMM-score: 15.6)
    KaiC domain protein, AF_0351 family (TIGR03880; HMM-score: 14.2)
  • TheSEED: see SA0642
  • PFAM:
    P-loop_NTPase (CL0023) cobW; CobW/HypB/UreG, nucleotide-binding domain (PF02492; HMM-score: 151.4)
    and 13 more
    no clan defined CobW_C; Cobalamin synthesis protein cobW C-terminal domain (PF07683; HMM-score: 52.4)
    P-loop_NTPase (CL0023) MobB; Molybdopterin guanine dinucleotide synthesis protein B (PF03205; HMM-score: 20.5)
    RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 17.5)
    ATPase_2; ATPase domain predominantly from Archaea (PF01637; HMM-score: 16.3)
    AAA_14; AAA domain (PF13173; HMM-score: 16.2)
    no clan defined DUF2824; Protein of unknown function (DUF2824) (PF11039; HMM-score: 14.9)
    P-loop_NTPase (CL0023) AAA_22; AAA domain (PF13401; HMM-score: 14.2)
    NACHT; NACHT domain (PF05729; HMM-score: 13.6)
    ATP_bind_1; Conserved hypothetical ATP binding protein (PF03029; HMM-score: 12.8)
    Arf; ADP-ribosylation factor family (PF00025; HMM-score: 12.5)
    ABC_tran; ABC transporter (PF00005; HMM-score: 12.3)
    T2SSE; Type II/IV secretion system protein (PF00437; HMM-score: 12.1)
    AAA_30; AAA domain (PF13604; HMM-score: 11.1)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.009718
    • TAT(Tat/SPI): 0.000283
    • LIPO(Sec/SPII): 0.001616
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKNNKDEKIRISIINGFLGSGKTTLLTHYISELLKNDEKIKIIMNEFGTFDIDSNSISNEIEVHSLINGCVCCDLKQELVYELKAIALKGDVNHVIIEATGIAHPLELLVACQDPQIVNFFEKPIIYGVLDATRFLERHQYTENTVSLMEEQLKLSDMIIINKIDLITDDSLEKIDKQLGMICASIPTYKTTYGKVSLEELDLTVKDREISSHHHHHHGIKSMTYTFTGPIDRQLFYQFIMKLPESVLRLKGYVSFRNQPNAIYEFQYAYGLPDYGIIGMQLPLTIVIIGETLDTNHIRNQLDMLQFT

Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]