From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS03910 [old locus tag: SACOL0761 ]
  • pan locus tag?: SAUPAN002586000
  • symbol: SACOL_RS03910
  • pan gene symbol?: nagA
  • synonym:
  • product: N-acetylglucosamine-6-phosphate deacetylase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS03910 [old locus tag: SACOL0761 ]
  • symbol: SACOL_RS03910
  • product: N-acetylglucosamine-6-phosphate deacetylase
  • replicon: chromosome
  • strand: +
  • coordinates: 782732..783913
  • length: 1182
  • essential: unknown other strains

Accession numbers[edit | edit source]

  • Location: NC_002951 (782732..783913) NCBI
  • BioCyc: SACOL_RS03910 BioCyc
  • MicrobesOnline: see SACOL0761

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    GTGTCAGAATTAATTATATATAACGGCAAAGTTTATACTGAAGATGGCAAAATCGATAAT
    GGTTACATTCATGTGAAAGATGGACAGATTGTTGCAATTGGAGAAGTGGATGATAAAGCA
    GCAATTGATAATGATACGACAAATAAAATTCAAGTGATTGATGCTAAAGGTCATCATGTA
    TTACCAGGTTTTATTGATATACATATTCATGGTGGTTATGGTCAAGATGCAATGGATGGG
    TCATACGATGGCTTAAAATATCTATCCGAAAATTTGTTGTCTGAAGGGACGACATCATAC
    TTGGCCACTACAATGACGCAATCGACTGATAAAATAGATAATGCACTTACAAATATTGCT
    AAATATGAAGCGGAGCAAGATGTTCACAATGCAGCGGAAATTGTAGGTATACATTTAGAA
    GGACCATTTATATCTGAAAATAAAGTTGGTGCTCAACATCCGCAATACGTTGTACGCCCA
    TTTATCGATAAAATTAAACATTTTCAAGAGACTGCTAACGGATTAATAAAGATTATGACG
    TTTGCACCTGAAGTTGAAGGTGCAAAAGAAGCGCTTGAAACGTATAAAGATGACATTATT
    TTTTCAATTGGTCATACAGTAGCAACATACGAAGAAGCAGTTGAAGCTGTTGAGCGAGGA
    GCTAAACATGTCACGCATTTATATAATGCAGCGACGCCATTCCAACATAGAGAACCAGGT
    GTTTTTGGAGCAGCATGGTTGAATGATGCTCTACATACCGAAATGATTGTTGATGGCACT
    CATTCTCATCCGGCATCGGTTGCAATTGCTTACCGTATGAAAGGTAATGAACGTTTTTAT
    TTAATTACCGATGCAATGCGTGCAAAAGGTATGCCTGAAGGAGAATATGATTTGGGTGGA
    CAAAAAGTAACTGTTCAATCGCAACAAGCACGTCTTGCAAATGGTGCGCTTGCTGGTAGT
    ATTTTAAAAATGAATCATGGGTTACGTAACTTAATATCATTTACAGGTGATACATTAGAT
    CATTTATGGCGAGTAACAAGTTTAAATCAAGCCATTGCATTAGGTATCGATGATAGAAAA
    GGTAGTATTAAAGTAAATAAGGATGCAGATCTTGTTATTCTAGATGATGATATGAATGTA
    AAATCTACAATAAAACAAGGCAAGGTTCACACATTTAGCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1182

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL_RS03910 [old locus tag: SACOL0761 ]
  • symbol: SACOL_RS03910
  • description: N-acetylglucosamine-6-phosphate deacetylase
  • length: 393
  • theoretical pI: 5.49488
  • theoretical MW: 43115.2
  • GRAVY: -0.302799

Function[edit | edit source]

  • reaction:
    EC 3.5.1.25?  ExPASy
    N-acetylglucosamine-6-phosphate deacetylase N-acetyl-D-glucosamine 6-phosphate + H2O = D-glucosamine 6-phosphate + acetate
  • TIGRFAM:
    Metabolism Central intermediary metabolism Amino sugars N-acetylglucosamine-6-phosphate deacetylase (TIGR00221; EC 3.5.1.25; HMM-score: 378.9)
    and 12 more
    allantoinase (TIGR03178; EC 3.5.2.5; HMM-score: 45.8)
    Metabolism Energy metabolism Amino acids and amines imidazolonepropionase (TIGR01224; EC 3.5.2.7; HMM-score: 44.2)
    dihydropyrimidinase (TIGR02033; EC 3.5.2.2; HMM-score: 42.9)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Other guanine deaminase (TIGR02967; EC 3.5.4.3; HMM-score: 41.1)
    Metabolism Energy metabolism Other phosphonate metabolism protein PhnM (TIGR02318; HMM-score: 39.6)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides beta-aspartyl peptidase (TIGR01975; EC 3.4.19.5; HMM-score: 36.3)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides adenine deaminase (TIGR01178; EC 3.5.4.2; HMM-score: 36.2)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis dihydroorotase, multifunctional complex type (TIGR00857; EC 3.5.2.3; HMM-score: 33.8)
    formylmethanofuran dehydrogenase subunit A (TIGR03121; EC 1.2.99.5; HMM-score: 26.4)
    Metabolism Central intermediary metabolism Nitrogen metabolism urease, alpha subunit (TIGR01792; EC 3.5.1.5; HMM-score: 19.7)
    putative selenium metabolism protein SsnA (TIGR03314; HMM-score: 17.8)
    Metabolism Energy metabolism Amino acids and amines formiminoglutamate deiminase (TIGR02022; EC 3.5.3.13; HMM-score: 13.8)
  • TheSEED: see SACOL0761
  • PFAM:
    Amidohydrolase (CL0034) Amidohydro_1; Amidohydrolase family (PF01979; HMM-score: 96.5)
    and 1 more
    Amidohydro_3; Amidohydrolase family (PF07969; HMM-score: 56.3)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: a divalent metal cation
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004913
    • TAT(Tat/SPI): 0.000179
    • LIPO(Sec/SPII): 0.000803
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MSELIIYNGKVYTEDGKIDNGYIHVKDGQIVAIGEVDDKAAIDNDTTNKIQVIDAKGHHVLPGFIDIHIHGGYGQDAMDGSYDGLKYLSENLLSEGTTSYLATTMTQSTDKIDNALTNIAKYEAEQDVHNAAEIVGIHLEGPFISENKVGAQHPQYVVRPFIDKIKHFQETANGLIKIMTFAPEVEGAKEALETYKDDIIFSIGHTVATYEEAVEAVERGAKHVTHLYNAATPFQHREPGVFGAAWLNDALHTEMIVDGTHSHPASVAIAYRMKGNERFYLITDAMRAKGMPEGEYDLGGQKVTVQSQQARLANGALAGSILKMNHGLRNLISFTGDTLDHLWRVTSLNQAIALGIDDRKGSIKVNKDADLVILDDDMNVKSTIKQGKVHTFS

Experimental data[edit | edit source]

  • experimentally validated: see SACOL0761
  • protein localization: see SACOL0761
  • quantitative data / protein copy number per cell: see SACOL0761
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]