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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS04045 [old locus tag: SACOL0787 ]
  • pan locus tag?: SAUPAN002621000
  • symbol: SACOL_RS04045
  • pan gene symbol?: bmrU
  • synonym:
  • product: lipid kinase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS04045 [old locus tag: SACOL0787 ]
  • symbol: SACOL_RS04045
  • product: lipid kinase
  • replicon: chromosome
  • strand: -
  • coordinates: 810154..811071
  • length: 918
  • essential: unknown other strains

Accession numbers[edit | edit source]

  • Location: NC_002951 (810154..811071) NCBI
  • BioCyc: SACOL_RS04045 BioCyc
  • MicrobesOnline: see SACOL0787

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGAAAATAAATATACACATGGTGTCCTCTTTTACCATGAACACAGTGGATTAAAAAAT
    ATTAATCAAGGTATTGGGGAAGTTACAACAGCATTAAGTTCAATTTGTAAGCATCTTTCT
    ATTCAATTGAGTGAAAACGAAGGTGACATTATTAAATATTGCCAAGAAATCAAAACTAAA
    AACTATGCAAAAGATGTAGATATATTATTTATTCTTGGCGGTGATGGCACGGTTAACGAA
    CTTATAAACGGTGTTATGACACATGACCTTCAACTCCCTATTGGCATTTTACCAGGCGGT
    ACTTTTAATGATTTTACAAAAACGCTAAATATAGCACCTAATCATAAACAAGCTAGTGAA
    CAAATGATTTCTGCACAAGTTGGTACATACGATGTAATTAAAATAAATAATCAATATGCA
    CTCAACTTTGTTGGCTTAGGTCTCATTGTTCAAAACGCTGAAAACGTACAAGATGGTTCA
    AAAGATATATTTGGTAAATTGAGTTATATTGGATCAACTGTCAAAACACTGCTAAATCCA
    ACACAATTTAATTATCAATTATCAATAGATGATAAGACATATTCTGGCGAAACGACCATG
    ATACTAACAGCAAATGGTCCATTCATTGGTGGTAGTCGTATACCATTGACTGATTTGTCT
    CCTCAAGATGGTGAACTGAACACTTTCATTTTTAATGAACAAAGTTTCAGTATTTTAAAT
    GATATTTTTAAAAAGCGTGATAGCATGAATTGGAATGAAATTACTCAAGGTATTGAGCAT
    ATACCTGGTAAAAAGATTTCACTTACAACAGATCCAGCAATGAAAGTTGATATTGATGGC
    GAAATTTCATTAGAAACACCTATTGATATTGAGGTTATACCAAATGCCATTCAGTTACTT
    ACTGTCAATGATTTGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    918

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL_RS04045 [old locus tag: SACOL0787 ]
  • symbol: SACOL_RS04045
  • description: lipid kinase
  • length: 305
  • theoretical pI: 4.49354
  • theoretical MW: 33625.9
  • GRAVY: -0.17082

Function[edit | edit source]

  • reaction:
    EC 2.7.1.-?  ExPASy
  • TIGRFAM:
    Unknown function Enzymes of unknown specificity lipid kinase, YegS/Rv2252/BmrU family (TIGR00147; EC 2.7.1.-; HMM-score: 168.7)
    and 1 more
    Unknown function Enzymes of unknown specificity lipid kinase YegS (TIGR03702; EC 2.7.1.-; HMM-score: 76.9)
  • TheSEED: see SACOL0787
  • PFAM:
    PFK (CL0240) DAGK_cat; Diacylglycerol kinase catalytic domain (PF00781; HMM-score: 93.1)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:

Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.016346
    • TAT(Tat/SPI): 0.00054
    • LIPO(Sec/SPII): 0.002097
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MENKYTHGVLFYHEHSGLKNINQGIGEVTTALSSICKHLSIQLSENEGDIIKYCQEIKTKNYAKDVDILFILGGDGTVNELINGVMTHDLQLPIGILPGGTFNDFTKTLNIAPNHKQASEQMISAQVGTYDVIKINNQYALNFVGLGLIVQNAENVQDGSKDIFGKLSYIGSTVKTLLNPTQFNYQLSIDDKTYSGETTMILTANGPFIGGSRIPLTDLSPQDGELNTFIFNEQSFSILNDIFKKRDSMNWNEITQGIEHIPGKKISLTTDPAMKVDIDGEISLETPIDIEVIPNAIQLLTVNDL

Experimental data[edit | edit source]

  • experimentally validated: see SACOL0787
  • protein localization: see SACOL0787
  • quantitative data / protein copy number per cell: see SACOL0787
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]