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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS04970 [old locus tag: SACOL0970 ]
  • pan locus tag?: SAUPAN003076000
  • symbol: SACOL_RS04970
  • pan gene symbol?: addB
  • synonym:
  • product: ATP-dependent helicase/deoxyribonuclease subunit B

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS04970 [old locus tag: SACOL0970 ]
  • symbol: SACOL_RS04970
  • product: ATP-dependent helicase/deoxyribonuclease subunit B
  • replicon: chromosome
  • strand: +
  • coordinates: 971982..975458
  • length: 3477
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (971982..975458) NCBI
  • BioCyc: SACOL_RS04970 BioCyc
  • MicrobesOnline: see SACOL0970

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
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    361
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    481
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    661
    721
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    841
    901
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    1021
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    1141
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    1321
    1381
    1441
    1501
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    1621
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    1981
    2041
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    3241
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    3421
    ATGACATTACATGCTTATTTAGGTAGAGCGGGAACAGGTAAGTCTACGAAAATGTTGACC
    GAAATAAAACAAAAAATGAAAGCAGATCCGCTTGGAGATCCAATCATTTTAATTGCGCCA
    ACTCAAAGTACATTTCAATTAGAACAAGCCTTTGTCAATGATCCGGAATTAAATGGTAGT
    TTAAGAACAGAAGTGTTGCATTTTGAACGATTAAGTCATCGTATTTTCCAAGAAGTTGGT
    AGTTATAGCGAACAAAAGTTATCTAAAGCTGCAACGGAAATGATGATTTATAACATTGTT
    CAAGAACAACAAAAGTATTTAAAACTTTATCAATCACAAGCAAAATATTATGGGTTTAGT
    GAAAAATTAACAGAACAAATTCAAGATTTTAAAAAATATGCAGTAACGCCTGAACATTTA
    GAACACTTTATTGCTGATAAAAATATGCAAACTCGAACTAAAAATAAGTTAGAGGATATT
    GCTTTAATATACCGTGAGTTCGAACAACGCATTCAAAACGAGTTTATTACGGGTGAGGAT
    TCATTACAATATTTTATTGATTGTATGCCGAAATCAGAGTGGCTAAAACGTGCTGATATA
    TATATTGATGGTTTTCACAACTTTTCAACGATTGAGTATTTAATAATCAAAGGATTAATT
    AAATATGCGAAGAGTGTCACAATTATATTGACGACAGATGGTAACCACGATCAATTTAGT
    TTATTTAGAAAACCATCGGAAGTGTTACGACATATTGAAGAAATAGCAAATGAACTCAAT
    ATTTCTATTGAACGTCAATATTTCAACCAATTATATCGCTTCAATAATCAAGATTTAAAG
    CATCTTGAACAAGAATTTGATGCACTTCAAATCAATCGAGTGGCATGTCAAGGTCATATC
    AATATTTTAGAATCTGCGACTATGAGAGAGGAAATAAATGAAATTGCGCGACGTATCATC
    GTTGATATTCGTGATAAGCAATTACGATATCAAGATATTGCTATTTTATATCGTGATGAA
    TCTTATGCTTATTTATTTGATTCCATATTACCGCTTTATAATATTCCTTATAATATTGAT
    ACAAAGCGTTCGATGACACATCATCCGGTCATGGAAATGATTCGTTCATTGATTGAAGTT
    ATTCAATCTAATTGGCAAGTGAATCCAATGCTACGCTTATTGAAGACTGATGTGTTAACG
    GCATCATATCTAAAAAGTGCATACTTAGTTGATTTACTTGAAAATTTTGTACTTGAACGT
    GGTATATACGGTAAACGTTGGTTAGATGATGAGCTATTTAATGTCGAACATTTTAGCAAA
    ATGGGGCGTAAAGGGCATAAACTGACCGAAGATGAACGTAACACATTTGAACAAGTCGTT
    AAGTTAAAGAAAGATGTCATTGATAAAATTTTACATTTTGAAAAGCAAATGTCACAAGCG
    GAAACTGTAAAAGATTTTGCAACTGCTTTTTATGAAAGTATGGAATATTTCGAACTGCCA
    AATCAATTGATGACAGAGCGAGATGAACTTGATTTAAATGGTAATCATGAAAAGGCGGAG
    GAAATTGATCAAATATGGAATGGCTTAATTCAAATCCTTGATGACTTAGTTCTAGTATTT
    GGAGATGAACCAATGTCGATGGAACGTTTCTTAGAAGTATTTGATATTGGTTTAGAACAA
    TTAGAATTTGTTATGATTCCGCAAACATTGGACCAAGTAAGTATTGGTACGATGGATTTG
    GCTAAAGTCGATAATAAGCAACATGTTTACTTAGTAGGTATGAATGATGGAACGATGCCA
    CAACCAGTAACTGCGTCAAGCTTGATTACAGATGAAGAAAAGAAATACTTTGAACAGCAG
    GCTAATGTCGAGTTAAGTCCAACATCAGATATTTTACAGATGGATGAAGCATTTGTTTGT
    TATGTTGCTATGACTAGAGCTAAGGGAGATGTTACATTTTCTTACAGTCTAATGGGATCA
    AGTGGTGATGATAAGGAGATCAGCCCATTTTTAAATCAAATTCAATCATTGTTCAACCAA
    TTGGAAATTACTAACATTCCTCAATACCATGAAGTTAACCCATTGTCACTAATGCAACAT
    GCTAAGCAAACCAAAATTACATTATTTGAAGCATTGCGTGCTTGGTTATATGATGAAATT
    GTGGCTGATAGTTGGTTAGATGCTTATCAAGTAATTAGAGATAGCGATCATTTAAATCAA
    GGTTTAGATTATTTAATGTCAGCATTAACGTTTGACAATGAAACTGTAAAATTAGGTGAA
    ACGTTGTCTAAAGATTTATATGGTAAGGAAATCAATGCCAGTGTATCCCGTTTTGAAGGT
    TATCAACAATGCCCATTTAAACACTATGCGTCACATGGTCTGAAACTAAATGAGCGAACG
    AAGTATGAACTTCAAAACTTTGATTTAGGTGATATTTTCCATTCTGTTTTAAAATATATA
    TCTGAACGTATTAATGGCGATTTTAAACAATTAGACCTGAAAAAAATAAGACAATTAACG
    AATGAAGCATTGGAAGAAATTTTACCTAAAGTTCAGTTTAATTTATTAAATTCTTCAGCT
    TACTATCGTTATTTATCAAGACGCATTGGCGCTATTGTAGAAACAACACTAAGCGCATTA
    AAATATCAAGGCACGTATTCAAAGTTTATGCCAAAACATTTTGAGACAAGTTTTAGAAGG
    AAACCAAGAACAAATGACGAATTAATTGCACAAACATTAACGACAACTCAAGGTATTCCA
    ATTAATATTAGAGGGCAAATTGACCGTATCGATACGTATACAAAGAATGATACAAGTTTT
    GTTAATATCATTGACTATAAATCCTCTGAAGGTAGTGCGACACTTGATTTAACGAAAGTA
    TATTATGGTATGCAAATGCAAATGATGACATACATGGATATCGTTTTACAAAATAAACAA
    CGCCTTGGATTAACAGATATTGTGAAACCAGGTGGATTATTATACTTCCATGTACATGAA
    CCTAGAATTAAATTTAAATCATGGTCTGATATTGATGAAGATAAACTAGAACAAGATTTA
    ATTAAAAAGTTTAAGTTGAGTGGTTTAGTTAATGCAGACCAAACTGTTATTGATGCATTG
    GATATTCGTTTAGAACCTAAATTCACTTCAGATATTGTACCAGTTGGTTTGAATAAAGAT
    GGCTCTTTGAGTAAACGAGGCAGCCAAGTGGCAGATGAAGCAACGATTTATAAATTCATC
    CAACATAACAAAGAGAATTTTATAGAAACAGCTTCAAATATTATGGATGGACATACTGAA
    GTTGCACCATTAAAGTACAAACAAAAATTGCCATGTGCTTTTTGTAGTTATCAATCGGTA
    TGTCATGTAGATGGCATGATTGATAGTAAGCGATATCGAACTGTAGATGAAACAATAAAT
    CCAATTGAAGCAATTCAAAATATTAACATTAATGATGAATTTGGGGGTGAGCAATAG
    60
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    3420
    3477

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL_RS04970 [old locus tag: SACOL0970 ]
  • symbol: SACOL_RS04970
  • description: ATP-dependent helicase/deoxyribonuclease subunit B
  • length: 1158
  • theoretical pI: 5.06875
  • theoretical MW: 134490
  • GRAVY: -0.423575

⊟Function[edit | edit source]

  • reaction:
    EC 3.6.4.12?  ExPASy
    DNA helicase ATP + H2O = ADP + phosphate
    EC 3.1.-.-?  ExPASy
  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair helicase-exonuclease AddAB, AddB subunit (TIGR02773; EC 3.1.-.-,3.6.4.12; HMM-score: 1417.2)
    and 5 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent nuclease subunit B (TIGR02774; HMM-score: 173.6)
    probable DNA repair protein (TIGR03623; HMM-score: 32.4)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase Rep (TIGR01074; EC 3.6.4.12; HMM-score: 30.1)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair double-strand break repair protein AddB (TIGR02786; HMM-score: 30)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase PcrA (TIGR01073; EC 3.6.4.12; HMM-score: 14.2)
  • TheSEED: see SACOL0970
  • PFAM:
    PDDEXK (CL0236) PDDEXK_1; PD-(D/E)XK nuclease superfamily (PF12705; HMM-score: 174.4)
    and 3 more
    P-loop_NTPase (CL0023) UvrD_C; UvrD-like helicase C-terminal domain (PF13361; HMM-score: 58.6)
    AAA_30; AAA domain (PF13604; HMM-score: 14.5)
    no clan defined Imm21; Immunity protein 21 (PF15589; HMM-score: 13)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+, [4Fe-4S] cluster
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002995
    • TAT(Tat/SPI): 0.000315
    • LIPO(Sec/SPII): 0.00049
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTLHAYLGRAGTGKSTKMLTEIKQKMKADPLGDPIILIAPTQSTFQLEQAFVNDPELNGSLRTEVLHFERLSHRIFQEVGSYSEQKLSKAATEMMIYNIVQEQQKYLKLYQSQAKYYGFSEKLTEQIQDFKKYAVTPEHLEHFIADKNMQTRTKNKLEDIALIYREFEQRIQNEFITGEDSLQYFIDCMPKSEWLKRADIYIDGFHNFSTIEYLIIKGLIKYAKSVTIILTTDGNHDQFSLFRKPSEVLRHIEEIANELNISIERQYFNQLYRFNNQDLKHLEQEFDALQINRVACQGHINILESATMREEINEIARRIIVDIRDKQLRYQDIAILYRDESYAYLFDSILPLYNIPYNIDTKRSMTHHPVMEMIRSLIEVIQSNWQVNPMLRLLKTDVLTASYLKSAYLVDLLENFVLERGIYGKRWLDDELFNVEHFSKMGRKGHKLTEDERNTFEQVVKLKKDVIDKILHFEKQMSQAETVKDFATAFYESMEYFELPNQLMTERDELDLNGNHEKAEEIDQIWNGLIQILDDLVLVFGDEPMSMERFLEVFDIGLEQLEFVMIPQTLDQVSIGTMDLAKVDNKQHVYLVGMNDGTMPQPVTASSLITDEEKKYFEQQANVELSPTSDILQMDEAFVCYVAMTRAKGDVTFSYSLMGSSGDDKEISPFLNQIQSLFNQLEITNIPQYHEVNPLSLMQHAKQTKITLFEALRAWLYDEIVADSWLDAYQVIRDSDHLNQGLDYLMSALTFDNETVKLGETLSKDLYGKEINASVSRFEGYQQCPFKHYASHGLKLNERTKYELQNFDLGDIFHSVLKYISERINGDFKQLDLKKIRQLTNEALEEILPKVQFNLLNSSAYYRYLSRRIGAIVETTLSALKYQGTYSKFMPKHFETSFRRKPRTNDELIAQTLTTTQGIPINIRGQIDRIDTYTKNDTSFVNIIDYKSSEGSATLDLTKVYYGMQMQMMTYMDIVLQNKQRLGLTDIVKPGGLLYFHVHEPRIKFKSWSDIDEDKLEQDLIKKFKLSGLVNADQTVIDALDIRLEPKFTSDIVPVGLNKDGSLSKRGSQVADEATIYKFIQHNKENFIETASNIMDGHTEVAPLKYKQKLPCAFCSYQSVCHVDGMIDSKRYRTVDETINPIEAIQNININDEFGGEQ

⊟Experimental data[edit | edit source]

  • experimentally validated: see SACOL0970
  • protein localization: see SACOL0970
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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