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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS05390 [old locus tag: SACOL1057 ]
  • pan locus tag?: SAUPAN003251000
  • symbol: SACOL_RS05390
  • pan gene symbol?: sspA
  • synonym:
  • product: serine protease

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS05390 [old locus tag: SACOL1057 ]
  • symbol: SACOL_RS05390
  • product: serine protease
  • replicon: chromosome
  • strand: -
  • coordinates: 1063016..1064026
  • length: 1011
  • essential: unknown other strains

Accession numbers[edit | edit source]

  • Location: NC_002951 (1063016..1064026) NCBI
  • BioCyc: SACOL_RS05390 BioCyc
  • MicrobesOnline: see SACOL1057

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAAGGTAAATTTTTAAAAGTTAGTTCTTTATTCGTTGCAACTTTGACAACAGCGACA
    CTTGTGAGTTCTCCAGCAGCAAACGCGTTATCTTCAAAGGCTATGGACAATCATCCACAA
    CAAACGCAGTCAAGCAAACAGCAAACACCTAAGATTCAAAAAGGCGGTAACCTTAAACCA
    TTAGAACAACGTGAACACGCAAATGTTATATTACCAAATAACGATCGTCACCAAATCACA
    GATACAACGAATGGTCATTATGCACCCGTAACTTATATTCAAGTTGAAGCACCTACTGGT
    ACATTTATTGCTTCCGGTGTAGTTGTAGGTAAAGATACTCTTTTAACAAATAAACACGTC
    GTAGATGCTACGCACGGTGATCCTCATGCTTTAAAAGCATTCCCTTCTGCAATTAACCAA
    GACAATTATCCAAATGGTGGTTTCACTGCTGAACAAATCACTAAATATTCAGGCGAAGGT
    GATTTAGCAATAGTTAAATTCTCCCCTAATGAGCAAAACAAACATATTGGTGAAGTAGTT
    AAACCAGCAACAATGAGTAATAATGCTGAAACACAAGTTAACCAAAATATTACTGTAACA
    GGATATCCTGGTGATAAACCTGTAGCAACAATGTGGGAAAGTAAAGGAAAAATCACTTAC
    CTCAAAGGCGAAGCTATGCAATATGATTTAAGTACAACTGGTGGTAATTCAGGTTCACCT
    GTATTTAATGAAAAAAATGAAGTGATCGGAATTCATTGGGGCGGTGTACCAAATGAATTT
    AATGGTGCGGTATTTATTAATGAAAATGTACGCAACTTCTTAAAACAAAATATTGAAGAT
    ATCCATTTTGCCAACGATGACCAACCTAATAACCCAGATAATCCTGATAACCCTAACAAT
    CCTGATAACCCTAACAACCCAGATGAACCAAATAACCCTGACAACCCTAACAACCCTGAT
    AATCCAGACAATGGCGATACCAATAATTCAGACAATCCAGATGCAGCTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1011

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL_RS05390 [old locus tag: SACOL1057 ]
  • symbol: SACOL_RS05390
  • description: serine protease
  • length: 336
  • theoretical pI: 4.80889
  • theoretical MW: 36312.4
  • GRAVY: -0.831548

Function[edit | edit source]

  • reaction:
    EC 3.4.21.19?  ExPASy
    Glutamyl endopeptidase Preferential cleavage: Glu-|-Xaa, Asp-|-Xaa
  • TIGRFAM:
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 18.1)
    Genetic information processing Protein fate Protein folding and stabilization peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 18.1)
    and 2 more
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides periplasmic serine peptidase DegS (TIGR02038; EC 3.4.21.-; HMM-score: 13.3)
    Signal transduction Regulatory functions Protein interactions periplasmic serine peptidase DegS (TIGR02038; EC 3.4.21.-; HMM-score: 13.3)
  • TheSEED: see SACOL1057
  • PFAM:
    Peptidase_PA (CL0124) Trypsin_2; Trypsin-like peptidase domain (PF13365; HMM-score: 75.1)
    and 7 more
    Trypsin; Trypsin (PF00089; HMM-score: 50.2)
    Peptidase_S46; Peptidase S46 (PF10459; HMM-score: 26.9)
    Peptidase_S7; Peptidase S7, Flavivirus NS3 serine protease (PF00949; HMM-score: 22.2)
    Peptidase_C3; 3C cysteine protease (picornain 3C) (PF00548; HMM-score: 18.4)
    Peptidase_S32; Equine arteritis virus serine endopeptidase S32 (PF05579; HMM-score: 17.2)
    Peptidase_S39; Peptidase S39 (PF02122; HMM-score: 16.1)
    Peptidase_CA (CL0125) Phytochelatin; Phytochelatin synthase (PF05023; HMM-score: 11.7)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.02
    • Extracellular Score: 9.98
    • Internal Helices: 0
  • LocateP:
  • SignalP: Signal peptide SP(Sec/SPI) length 29 aa
    • SP(Sec/SPI): 0.969793
    • TAT(Tat/SPI): 0.025019
    • LIPO(Sec/SPII): 0.004285
    • Cleavage Site: CS pos: 29-30. ANA-LS. Pr: 0.6936
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKGKFLKVSSLFVATLTTATLVSSPAANALSSKAMDNHPQQTQSSKQQTPKIQKGGNLKPLEQREHANVILPNNDRHQITDTTNGHYAPVTYIQVEAPTGTFIASGVVVGKDTLLTNKHVVDATHGDPHALKAFPSAINQDNYPNGGFTAEQITKYSGEGDLAIVKFSPNEQNKHIGEVVKPATMSNNAETQVNQNITVTGYPGDKPVATMWESKGKITYLKGEAMQYDLSTTGGNSGSPVFNEKNEVIGIHWGGVPNEFNGAVFINENVRNFLKQNIEDIHFANDDQPNNPDNPDNPNNPDNPNNPDEPNNPDNPNNPDNPDNGDTNNSDNPDAA

Experimental data[edit | edit source]

  • experimentally validated: see SACOL1057
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]