PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0951 [new locus tag: SAUSA300_RS05110 ]
• pan locus tag^?: SAUPAN003251000
• symbol: sspA
• pan gene symbol^?: sspA
• synonym:
• product: V8 protease

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0951 [new locus tag: SAUSA300_RS05110 ]
• symbol: sspA
• product: V8 protease
• replicon: chromosome
• strand: -
• coordinates: 1039542..1040552
• length: 1011
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913747 NCBI
• RefSeq: YP_493649 NCBI
• BioCyc: see SAUSA300_RS05110
• MicrobesOnline: 1292466 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAAAGGTAAATTTTTAAAAGTTAGTTCTTTATTCGTTGCAACTTTGACAACAGCGACA
  CTTGTGAGTTCTCCAGCAGCAAACGCGTTATCTTCAAAGGCTATGGACAATCATCCACAA
  CAAACGCAGTCAAGCAAACAGCAAACACCTAAGATTCAAAAAGGCGGTAACCTTAAACCA
  TTAGAACAACGTGAACACGCAAATGTTATATTACCAAATAACGATCGTCACCAAATCACA
  GATACAACGAATGGTCATTATGCACCCGTAACTTATATTCAAGTTGAAGCACCTACTGGT
  ACATTTATTGCTTCCGGTGTAGTTGTAGGTAAAGATACTCTTTTAACAAATAAACACGTC
  GTAGATGCTACGCACGGTGATCCTCATGCTTTAAAAGCATTCCCTTCTGCAATTAACCAA
  GACAATTATCCAAATGGTGGTTTCACTGCTGAACAAATCACTAAATATTCAGGCGAAGGT
  GATTTAGCAATAGTTAAATTCTCCCCTAATGAGCAAAACAAACATATTGGTGAAGTAGTT
  AAACCAGCAACAATGAGTAATAATGCTGAAACACAAGTTAACCAAAATATTACTGTAACA
  GGATATCCTGGTGATAAACCTGTAGCAACAATGTGGGAAAGTAAAGGAAAAATCACTTAC
  CTCAAAGGCGAAGCTATGCAATATGATTTAAGTACAACTGGTGGTAATTCAGGTTCACCT
  GTATTTAATGAAAAAAATGAAGTGATCGGAATTCATTGGGGCGGTGTACCAAATGAATTT
  AATGGTGCGGTATTTATTAATGAAAATGTACGCAACTTCTTAAAACAAAATATTGAAGAT
  ATCCATTTTGCCAACGATGACCAACCTAATAACCCAGATAATCCTGATAACCCTAACAAT
  CCTGATAACCCTAACAACCCAGATGAACCAAATAACCCTGACAACCCTAACAACCCTGAT
  AATCCAGACAATGGCGATAACAATAATTCAGACAATCCAGATGCAGCTTAA
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  1011
  

⊟Protein

⊟General

• locus tag: SAUSA300_0951 [new locus tag: SAUSA300_RS05110 ]
• symbol: SspA
• description: V8 protease
• length: 336
• theoretical pI: 4.80889
• theoretical MW: 36325.4
• GRAVY: -0.839881

⊟Function

• reaction:
  EC 3.4.21.19?  ExPASy
  Glutamyl endopeptidase Preferential cleavage: Glu-|-Xaa, Asp-|-Xaa
• TIGRFAM:
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 18.1)
  Genetic information processing Protein fate Protein folding and stabilization peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 18.1)
  and 2 more
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides periplasmic serine peptidase DegS (TIGR02038; EC 3.4.21.-; HMM-score: 13.3)
  Signal transduction Regulatory functions Protein interactions periplasmic serine peptidase DegS (TIGR02038; EC 3.4.21.-; HMM-score: 13.3)
• TheSEED  :
  • Glutamyl endopeptidase precursor (EC 3.4.21.19), serine proteinase SspA
  Protein Metabolism Protein degradation EC 3.4.21.- Serine endopeptidase  Glutamyl endopeptidase precursor (EC 3.4.21.19), serine proteinase SspA
• PFAM:
  Peptidase_PA (CL0124) Trypsin_2; Trypsin-like peptidase domain (PF13365; HMM-score: 75.1)
  and 7 more
  Trypsin; Trypsin (PF00089; HMM-score: 50.2)
  Peptidase_S46; Peptidase S46 (PF10459; HMM-score: 26.9)
  Peptidase_S7; Peptidase S7, Flavivirus NS3 serine protease (PF00949; HMM-score: 22.2)
  Peptidase_C3; 3C cysteine protease (picornain 3C) (PF00548; HMM-score: 18.4)
  Peptidase_S32; Equine arteritis virus serine endopeptidase S32 (PF05579; HMM-score: 17.2)
  Peptidase_S39; Peptidase S39 (PF02122; HMM-score: 16.1)
  Peptidase_CA (CL0125) Phytochelatin; Phytochelatin synthase (PF05023; HMM-score: 11.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Extracellular
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0
  • Extracellular Score: 10
  • Internal Helices: 0
• LocateP: Secretory(released) (with CS)
  • Prediction by SwissProt Classification: Extracellular
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: -0.17
  • Signal peptide possibility: 1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: VSSPAANA
• SignalP: Signal peptide SP(Sec/SPI) length 29 aa
  • SP(Sec/SPI): 0.969793
  • TAT(Tat/SPI): 0.025019
  • LIPO(Sec/SPII): 0.004285
  • Cleavage Site: CS pos: 29-30. ANA-LS. Pr: 0.6936
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160772 NCBI
• RefSeq: YP_493649 NCBI
• UniProt: A0A0H2XFC3 UniProt

⊟Protein sequence

• MKGKFLKVSSLFVATLTTATLVSSPAANALSSKAMDNHPQQTQSSKQQTPKIQKGGNLKPLEQREHANVILPNNDRHQITDTTNGHYAPVTYIQVEAPTGTFIASGVVVGKDTLLTNKHVVDATHGDPHALKAFPSAINQDNYPNGGFTAEQITKYSGEGDLAIVKFSPNEQNKHIGEVVKPATMSNNAETQVNQNITVTGYPGDKPVATMWESKGKITYLKGEAMQYDLSTTGGNSGSPVFNEKNEVIGIHWGGVPNEFNGAVFINENVRNFLKQNIEDIHFANDDQPNNPDNPDNPNNPDNPNNPDEPNNPDNPNNPDNPDNGDNNNSDNPDAA

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: sspC < sspB < sspA

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications