From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS05850 [old locus tag: SACOL1146 ]
  • pan locus tag?: SAUPAN003368000
  • symbol: SACOL_RS05850
  • pan gene symbol?: isdG
  • synonym:
  • product: monooxygenase IsdI

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS05850 [old locus tag: SACOL1146 ]
  • symbol: SACOL_RS05850
  • product: monooxygenase IsdI
  • replicon: chromosome
  • strand: +
  • coordinates: 1153717..1154040
  • length: 324
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (1153717..1154040) NCBI
  • BioCyc: SACOL_RS05850 BioCyc
  • MicrobesOnline: see SACOL1146

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    ATGAAATTTATGGCAGAAAATAGGCTGACGTTAACAAAAGGAACAGCAAAAGATATTATA
    GAACGATTTTACACGAGACATGGGATTGAAACATTAGAAGGCTTTGATGGCATGTTTGTT
    ACACAAACTTTAGAACAAGAAGATTTTGATGAAGTGAAAATTTTAACAGTTTGGAAATCA
    AAGCAAGCTTTTACGGATTGGTTAAAATCTGATGTCTTTAAAGCAGCGCATAAACATGTT
    AGAAGTAAAAATGAAGATGAAAGTAGCCCGATTATAAATAACAAAGTAATTACATATGAT
    ATAGGCTATAGTTACATGAAATAA
    60
    120
    180
    240
    300
    324

⊟Protein[edit | edit source]

Protein Data Bank: 2ZDO

⊟General[edit | edit source]

  • locus tag: SACOL_RS05850 [old locus tag: SACOL1146 ]
  • symbol: SACOL_RS05850
  • description: monooxygenase IsdI
  • length: 107
  • theoretical pI: 6.80692
  • theoretical MW: 12546.2
  • GRAVY: -0.566355

⊟Function[edit | edit source]

  • reaction:
    EC 1.14.99.3?  ExPASy
    Transferred entry: 1.14.14.18
    EC 1.14.99.48?  ExPASy
    Heme oxygenase (staphylobilin-producing) Protoheme + 5 reduced acceptor + 4 O2 = 5-oxo-delta-bilirubin + Fe2+ + formaldehyde + 5 acceptor + 4 H2O Protoheme + 5 reduced acceptor + 4 O2 = 15-oxo-beta-bilirubin + Fe2+ + formaldehyde + 5 acceptor + 4 H2O
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Aspartate family homoserine O-succinyltransferase (TIGR01001; EC 2.3.1.46; HMM-score: 15.8)
  • TheSEED: see SACOL1146
  • PFAM:
    Dim_A_B_barrel (CL0032) ABM; Antibiotic biosynthesis monooxygenase (PF03992; HMM-score: 53)
    and 2 more
    Glutaminase_I (CL0014) HTS; Homoserine O-succinyltransferase (PF04204; HMM-score: 15)
    Dim_A_B_barrel (CL0032) Dehydratase_hem; Haem-containing dehydratase (PF13816; HMM-score: 14.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 10
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002024
    • TAT(Tat/SPI): 0.000226
    • LIPO(Sec/SPII): 0.000347
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKFMAENRLTLTKGTAKDIIERFYTRHGIETLEGFDGMFVTQTLEQEDFDEVKILTVWKSKQAFTDWLKSDVFKAAHKHVRSKNEDESSPIINNKVITYDIGYSYMK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SACOL_RS05850&oldid=90553"