From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS06530 [old locus tag: SACOL1279 ]
  • pan locus tag?: SAUPAN003561000
  • symbol: SACOL_RS06530
  • pan gene symbol?: uppS
  • synonym:
  • product: isoprenyl transferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS06530 [old locus tag: SACOL1279 ]
  • symbol: SACOL_RS06530
  • product: isoprenyl transferase
  • replicon: chromosome
  • strand: +
  • coordinates: 1288230..1289000
  • length: 771
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (1288230..1289000) NCBI
  • BioCyc: SACOL_RS06530 BioCyc
  • MicrobesOnline: see SACOL1279

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGTTTAAAAAGCTAATAAATAAAAAGAACACTATAAATAATTATAATGAAGAATTAGAC
    TCGTCTAATATACCTGAACATATCGCTATTATTATGGATGGTAATGGGCGATGGGCTAAG
    AAGCGAAAAATGCCTAGAATTAAAGGTCATTACGAAGGTATGCAAACAATAAAAAAAATT
    ACTAGGGTAGCTAGTGATATTGGTGTTAAGTACTTAACTTTATACGCCTTTTCCACTGAA
    AATTGGTCAAGACCTGAAAGTGAAGTAAATTATATTATGAATTTGCCTGTCAATTTCTTA
    AAGACATTCTTACCGGAACTAATTGAAAAAAATGTCAAAGTTGAAACAATTGGATTTACT
    GATAAGTTGCCAAAATCAACGATAGAAGCAATTAATAATGCTAAAGAAAAGACAGCTAAT
    AATACCGGCTTAAAATTAATATTTGCAATTAATTATGGTGGCAGAGCAGAACTTGTTCAT
    AGTATTAAAAATATGTTTGACGAGCTTCATCAACAAGGTTTAAATAGTGATATCATAGAT
    GAAACATATATAAACAATCATTTAATGACAAAAGACTATCCTGATCCAGAGTTGTTAATT
    CGTACTTCAGGAGAACAAAGAATAAGTAATTTCTTGATTTGGCAAGTTTCGTATAGTGAA
    TTTATCTTTAATCAAAAATTATGGCCTGACTTTGACGAAGATGAATTAATTAAATGTATA
    AAAATTTATCAGTCACGTCAAAGACGCTTTGGCGGATTGAGTGAGGAGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    771

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL_RS06530 [old locus tag: SACOL1279 ]
  • symbol: SACOL_RS06530
  • description: isoprenyl transferase
  • length: 256
  • theoretical pI: 8.36963
  • theoretical MW: 29848
  • GRAVY: -0.545313

⊟Function[edit | edit source]

  • reaction:
    EC 2.5.1.31?  ExPASy
    Ditrans,polycis-undecaprenyl-diphosphate synthase ((2E,6E)-farnesyl-diphosphate specific) (2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate = 8 diphosphate + di-trans,octa-cis-undecaprenyl diphosphate
  • TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides di-trans,poly-cis-decaprenylcistransferase (TIGR00055; EC 2.5.1.31; HMM-score: 274.9)
  • TheSEED: see SACOL1279
  • PFAM:
    no clan defined Prenyltransf; Putative undecaprenyl diphosphate synthase (PF01255; HMM-score: 267.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.009034
    • TAT(Tat/SPI): 0.000506
    • LIPO(Sec/SPII): 0.000784
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MFKKLINKKNTINNYNEELDSSNIPEHIAIIMDGNGRWAKKRKMPRIKGHYEGMQTIKKITRVASDIGVKYLTLYAFSTENWSRPESEVNYIMNLPVNFLKTFLPELIEKNVKVETIGFTDKLPKSTIEAINNAKEKTANNTGLKLIFAINYGGRAELVHSIKNMFDELHQQGLNSDIIDETYINNHLMTKDYPDPELLIRTSGEQRISNFLIWQVSYSEFIFNQKLWPDFDEDELIKCIKIYQSRQRRFGGLSEE

⊟Experimental data[edit | edit source]

  • experimentally validated: see SACOL1279
  • protein localization: see SACOL1279
  • quantitative data / protein copy number per cell: see SACOL1279
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SACOL_RS06530&oldid=97073"