PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS07225 [old locus tag: SACOL1419 ]
• pan locus tag^?: SAUPAN003790000
• symbol: SACOL_RS07225
• pan gene symbol^?: —
• synonym:
• product: oligoendopeptidase F

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS07225 [old locus tag: SACOL1419 ]
• symbol: SACOL_RS07225
• product: oligoendopeptidase F
• replicon: chromosome
• strand: +
• coordinates: 1429600..1431414
• length: 1815
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (1429600..1431414) NCBI
• BioCyc: SACOL_RS07225 BioCyc
• MicrobesOnline: see SACOL1419

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGTCTCAAGGTTTACCTTTAAGAGAAGATGTTCCTGTTTCAGAAACATGGGATTTAGTA
  GACTTATTTAAAGATGATCAACAATATTATGAAAGTATTGACGCTCTAGTACAACAAGCA
  AATCAATTTCATCATACATATGCAACAACATTAAATTCAATCGAACAAATTAATACTGCT
  TTAGCTGAATTAGAAAATATTTTAATTGCCTTAGATCGCTTAAGTAATTATGCAGAACTA
  CGTTTAAGTGTAGATACTAGTAATATCGAGGCACAAGTATTGAGCGCTAAATTATCTACT
  ACATACGGTAAAATTGTTAGCCAATTATCATTTGTAGAGTCAGAAATACTTGAATTACCA
  GAAGAAATACTTCAACAATTAGAAGAATCATGTCCATATCAACACTATATTAAACAGTTA
  ATAAAACAAAAGCCATTCCAATTATCTGCGTCGGTAGAACAAGTATTAGCAACTTTATCA
  CCTACGCTAAACAGTCCTTACGATTTATACGGCACGACAAAAATGCTAGATATTACATTC
  GATTCATTTGAACATGATGGTACAACGTACCCTGTCGACTATGCTACGTTTGAAAATGAT
  TATGAAGATAATAAAGATCCTGAGTTTAGACGTAAAAGTTTCAAATCGTTTAGCGATGGG
  ATTCGAAAATATCAGCATACTACCGCGGCTACATATAATATGCAAGTACAACAAGAAAAA
  ATTGAAGCTGATTTACGTGGATTTGAATCAGTCATCGATTATTTATTACATAGTCAAGAA
  GTAACGCGTGATATGTTTGACCGTCAAATCGATATGATTATGCGTGACTTGGCACCAGTT
  ATGCAGAAATATGCTAAACTTTTACAACGTATTCACGGATTAGATAACATGCGTTTTGAA
  GACTTGAAGATTTCTGTAGACCCTGATTATGAACCAGAGATTTCAATTGAAGACTCAAAA
  AATTATATTTTCGGTGCGTTAAGTGTTTTAGGTGATGACTATACAAACATGTTACGTGAA
  GCATACGATCAGCGATGGATTGATTTTGCACAAAATAAAGGTAAAGATACAGGCGCATTT
  TGTGCAAGTCCATACTTTACACATTCATATGTGTTTATTTCTTGGACTGGTAAAATGGCT
  GAAGCATTTGTCTTAGCACATGAATTAGGTCATGCAGGTCATTTTACATTAGCTCAAAAA
  CATCAACCATATCTTGAATCAGAAGCATCAATGTACTTTGTTGAAGCCCCTTCTACAATG
  AATGAAATGTTGATGGCCAATTATTTATTTAACACAAGTGATAATCCAAGATTTAAGCGT
  TGGATTATTGGCTCAATTTTATCTAGAACATATTATCATAATATGGTTACCCATTTATTA
  GAAGCTGCTTATCAACGTGAAGTGTATCACAAAGTAGATCAAGGTGAATCTTTAAATGCG
  CCGACATTAAATGAAATAATGCTAAATGTTTATAAACAATTTTTTGGAGATGCAGTAGAC
  ATGACTGAGGGTGCTGAATTAACATGGATGCGTCAACCTCATTACTATATGGGATTATAT
  TCGTATACGTATTCTGCTGGCTTAACAATCGGAACTGTCGTTTCTCAAAAGATTAAAAAT
  GAAGGCCAACCAGCTGTTGATGCTTGGTTAGAAACATTGAAAAAAGGTGGTAGTGTATCA
  CCTGTCGAACTTGCAAACATTGCAGGTGTAGACATTACTACAGAACAGCCACTTAAATCT
  ACAATTCAATATATTTCTGATTTAGTCGATGAAGTTGAAAAATTAACAGATGAAATTGAG
  CAAGCAAATAACTAA
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⊟Protein

⊟General

• locus tag: SACOL_RS07225 [old locus tag: SACOL1419 ]
• symbol: SACOL_RS07225
• description: oligoendopeptidase F
• length: 604
• theoretical pI: 4.38757
• theoretical MW: 69298.3
• GRAVY: -0.401656

⊟Function

• TIGRFAM:
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides oligoendopeptidase F (TIGR00181; EC 3.4.24.-; HMM-score: 721.6)
  and 2 more
  oligoendopeptidase, pepF/M3 family (TIGR02290; EC 3.4.24.-; HMM-score: 283.6)
  oligoendopeptidase, M3 family (TIGR02289; EC 3.4.24.-; HMM-score: 66.3)
• TheSEED: see SACOL1419
• PFAM:
  Peptidase_MA (CL0126) Peptidase_M3; Peptidase family M3 (PF01432; HMM-score: 124.8)
  and 4 more
  no clan defined Peptidase_M3_N; Oligopeptidase F (PF08439; HMM-score: 54.1)
  BLOC1_2; Biogenesis of lysosome-related organelles complex-1 subunit 2 (PF10046; HMM-score: 8.9)
  LOH1CR12; Tumour suppressor protein (PF10158; HMM-score: 8.3)
  EsxAB (CL0352) WXG100; Proteins of 100 residues with WXG (PF06013; HMM-score: 6.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: Zn²⁺
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001997
  • TAT(Tat/SPI): 0.000547
  • LIPO(Sec/SPII): 0.000272
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 445999714 NCBI
• RefSeq: WP_000077569 NCBI
• UniProt: see SACOL1419

⊟Protein sequence

• MSQGLPLREDVPVSETWDLVDLFKDDQQYYESIDALVQQANQFHHTYATTLNSIEQINTALAELENILIALDRLSNYAELRLSVDTSNIEAQVLSAKLSTTYGKIVSQLSFVESEILELPEEILQQLEESCPYQHYIKQLIKQKPFQLSASVEQVLATLSPTLNSPYDLYGTTKMLDITFDSFEHDGTTYPVDYATFENDYEDNKDPEFRRKSFKSFSDGIRKYQHTTAATYNMQVQQEKIEADLRGFESVIDYLLHSQEVTRDMFDRQIDMIMRDLAPVMQKYAKLLQRIHGLDNMRFEDLKISVDPDYEPEISIEDSKNYIFGALSVLGDDYTNMLREAYDQRWIDFAQNKGKDTGAFCASPYFTHSYVFISWTGKMAEAFVLAHELGHAGHFTLAQKHQPYLESEASMYFVEAPSTMNEMLMANYLFNTSDNPRFKRWIIGSILSRTYYHNMVTHLLEAAYQREVYHKVDQGESLNAPTLNEIMLNVYKQFFGDAVDMTEGAELTWMRQPHYYMGLYSYTYSAGLTIGTVVSQKIKNEGQPAVDAWLETLKKGGSVSPVELANIAGVDITTEQPLKSTIQYISDLVDEVEKLTDEIEQANN

⊟Experimental data

• experimentally validated: see SACOL1419
• protein localization: see SACOL1419
• quantitative data / protein copy number per cell: see SACOL1419
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: see SACOL1419

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications