PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS07540 [old locus tag: SACOL1480 ]
• pan locus tag^?: SAUPAN003895000
• symbol: SACOL_RS07540
• pan gene symbol^?: dynA
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS07540 [old locus tag: SACOL1480 ]
• symbol: SACOL_RS07540
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 1518288..1521728
• length: 3441
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (1518288..1521728) NCBI
• BioCyc: SACOL_RS07540 BioCyc
• MicrobesOnline: see SACOL1480

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGATTAATAAAGAACAATTAGATCTTTTATATAAATTAAAAAAAGAAGTTGAAAAGTCG
  CGAAATGAAGCACTTTTACATACAATTAACCAAGTAATTAAGAAAGTATATTTGCAGCAA
  TATACATGTTCGTTCGTTGGACATTTTTCTGCAGGTAAATCGACACTGATAAATTTATTA
  ATTGAACAAGATATCTTACCAAGTTCACCTGTACCAACGACAAGTAATACTGCTATTGTG
  TCAGTTTCAGACAATCACGATATTATTGCTAATTTGCCGAATCAAACGTATGCCAAATTA
  TCTAATTATGATGAAGTAAGGGAAATGAATCGCCAAAATGTCGACGTTGAATCTGTAGAA
  ATTAATTTTCAATCAGCTAAATTTGAAAATGGGTTTACGTTGCAAGATACACCAGGTGTT
  GATTCAAATGTTGCATCACATCAGTCAATAACAGAACAATATATGTATACAAGTAATATG
  ATATTTTATACGGTTGACTATAACCACGTTCAATCTGAACTTAACTTTAAGTTTATGAAG
  CATATAAATGATGTTGGAATACCTGTTGTGTTTATCATTAATCAAATTGACAAGCATCAA
  GACGATGAATTGTCATTCTCTACGTTTAAATCTCGAGTTGAAAAATCAATTGCAGATTGG
  GGTATTAAATTAGAACGCACCTTTTATGTATCTAAATTTGATCACCCTGAAAATGAACTT
  GAAGTATTATCAAGTTATCTAATTTCATTAGATCAACATAGAGAGACAATAGAGGATTAT
  ACATCAAGAACGGTTGAATACATTACCGAAGCTCAGCTAGATTACATTCAGTCTGAAATT
  CAGGAAGTACTAGAAGATTTAGGTATCGAAGAAGCGGAGTTTGAACAAGCATTTTTAAAT
  AGTCAACAACATCAAGCAATTAGTGAAGAGGCACAACTTTTAAATAATCCAGATGAATTA
  ATGGCATTTTTAAAGAATAAGCGTAAAAATATTTTAGAAAATGCATACATTATGCCGCAT
  AATATGAGAGAAATGTTACGAAGTTATTTGGAAAGTATGTCTCAAGACTTTAATGTTGGC
  GGATTTTTTAATAAAAAGAAGAAAAAACTACAAATTCAACAACAGCGATTATTAACAGCG
  ACAGATGCGTTACAAGAACATGTTAATCAACAAATTCGTCAACCAATGCGAGAAGATATG
  TCATTTGTTACGCGTTTTATCAATAAAAAAGAAGCTTCAGATAAAGTATTAAATCAGCAT
  TATGACGTTAAGCCAGAAATGATTGAAGGTTTATATCAACCACAAACATCAATCAGCAAT
  ACTTATGTACTTACATTTTCAGACGAAGTGGTTAAAGCCATTAAGAAATATGTTGAACAA
  CAATCAACACCAATTTTTAAAGAAATAATAGAAAATGTGCAGGCAGATGAATTACCAACA
  GAAGAAAGTGATGATTTAAAAGAATATCAACGTTATACAGAATTAAATGAGCTGCGTCAG
  TCATTGACGACTAAGAATTATCGTCACTACTATATTCATTTAGATGAATCTCTAGATAAA
  TTAATAGGTCGACAAGAGACAACATATCAAGTGGCTACTGATAATGCTCAGGATAATCGT
  GATAATCAGCAGCTAAATCAAAATACAGCTACAACAAATATGTCTATAGATATTCAAAAA
  GCGCTTGATATAATTTCGGATGTGCCTTTGTTCAAGCGTACAAAGCAAGATATCCACGAA
  ACATTAACACGTATAGATAATAAATTAATAAAAATAGGTGTATTTGGAACATTTAGTGCT
  GGTAAAAGTAGTTTGATAAATGCATTATTAGGCGAGCATATTTTAGTCAGTTCTCCAAAT
  CCTACTACAGCAGCTACTACAGAAATATCTTATGGAGACGAGAGTTATATAACGCTAAAA
  TCACAATCGCAATTATTAGATGAAATTAATGCAGTAGTTGAATACCAAGATATGTCTTAT
  TCGACTATAGAAGACTTTATTAATTCAGATTTAGAAAAGTTAAAATCACATTTAAATAAA
  AATCAACTCGCTTTTATTCAGGCAGTTGAAAAACATTATAAATTGTATGTCAATATGTTG
  GAAAATGGAGAAAAACATGCCATTAATCAACAGGAATTGAAAAAGTGGAGTGCAGAAGAT
  GAATATGCAACATTTGTTAAGACAGTACACATTGCATTAATGCATGATTGGTTAAAAGGT
  AAAATAATTGTTGATTCATTAGGGCTACACTCAAATAACCAAAGGCATACAAATGAAACC
  GAACAAATTTTAACTTCTTCAGACTTAATATTGTATGTAAGTTATTTTAATCATTCATTT
  ACTGATAATGACAAAGCGTTTATAGAACACATGAAAGATATGAACCAGTTGAATGAAAAC
  CAAGCATTTAAAATGGTAATTAATGCTGCTGATTTAGCAGAAAGTCAAGATGATCTTGAA
  GCAGTTGAAACATATGTATCAGATGCATTAAGACAAGTACACTTACAATCAGACATTTTT
  GCTGTATCAAGTCGAAATGCATTGCAAGCTGAAGATAAGGGCATTGATCAATTAAAACAA
  AGCATACAACAATTTGTTGATGTTGAATCTAAATCAATTTTAGAACAACAAATGATTCAT
  CAGCTTCAACAAATGGATCGTTCTTATGTAGAGATGATTACAGAATTTGAAACAAATAAA
  GCTGATATTTCACGTCGCCAACAAAGGTTAACAGATTATAAAGATAAACAGCGTTTACAA
  CATCAATTAATTGATGCGACGTTACAACATACAGACAACGAAGTTGAAGAACAAGTTTAT
  CATTTAAATGCCCGTTTAAAACTACAACTACTTGACGATGTTAAATCAGTGTTTAATTCT
  CAAATGACGCAAAATAGTGATTTTAATGAAGAAAAGAAAGTGTCTACGAGAGTATACTTA
  GATCAAATTCATCAACGATTGTTTTTAGAGCAATCTTTAATTACAGAACGTATAAAAAAA
  TACTTTAATAAGCAACTCACTGAGCAAATTGCACCAATCGTTCAACAATTAGCAGATTTA
  CATGTCATTATTAATCCTCAGTTTAACTTTGAATCAGCTAATATAGAGCAACCATTATTG
  CACATCGATTTCAACGATATGCTAAATGCATTGCCTAAACAATTAACAAAACGTAAAATT
  TTGAATCCAAATGGGCAAAGAGATATACATGAATCAATTTGTCAAAGTACGTTAGGATTA
  TTACAACCACAAATGGGATTATTGAGGCAACAGCTTGAATTATATGTAAAGCAAATGGCT
  GTAGAAGCTGAATCGCAATTTGAAAGTTTTGAAGCTAATATTCAAACGCAAATAAACGAT
  TTATTAGCATTTGATTTAGATACAACACTTATCAATCAATTGAAAGATAAACATCAACAA
  CTGAAAACTATTTTATATTAA
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⊟Protein

⊟General

• locus tag: SACOL_RS07540 [old locus tag: SACOL1480 ]
• symbol: SACOL_RS07540
• description: hypothetical protein
• length: 1146
• theoretical pI: 4.81559
• theoretical MW: 133107
• GRAVY: -0.568063

⊟Function

• TIGRFAM:
  Genetic information processing Protein synthesis Other ribosome-associated GTPase EngA (TIGR03594; HMM-score: 67.4)
  Unknown function General small GTP-binding protein domain (TIGR00231; HMM-score: 55.5)
  and 4 more
  Genetic information processing Protein fate Protein modification and repair [FeFe] hydrogenase H-cluster maturation GTPase HydF (TIGR03918; HMM-score: 50)
  Genetic information processing Protein synthesis Other GTP-binding protein Era (TIGR00436; HMM-score: 28.5)
  Genetic information processing Protein synthesis Other ribosome biogenesis GTP-binding protein YsxC (TIGR03598; HMM-score: 28.1)
  Metabolism Energy metabolism Amino acids and amines ethanolamine utilization protein, EutP (TIGR02528; HMM-score: 19)
• TheSEED: see SACOL1480
• PFAM:
  P-loop_NTPase (CL0023) Dynamin_N; Dynamin family (PF00350; HMM-score: 209.6)
  and 9 more
  MMR_HSR1; 50S ribosome-binding GTPase (PF01926; HMM-score: 63.2)
  FeoB_N; Ferrous iron transport protein B (PF02421; HMM-score: 26.4)
  RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 25.5)
  GTP_EFTU; Elongation factor Tu GTP binding domain (PF00009; HMM-score: 20.2)
  ArgK; ArgK protein (PF03308; HMM-score: 14.9)
  AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 13.5)
  no clan defined E1_FCCH; Ubiquitin-activating enzyme E1 FCCH domain (PF16190; HMM-score: 12.9)
  P-loop_NTPase (CL0023) AAA_18; AAA domain (PF13238; HMM-score: 11.9)
  AAA_28; AAA domain (PF13521; HMM-score: 6.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.012476
  • TAT(Tat/SPI): 0.001
  • LIPO(Sec/SPII): 0.001018
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446531175 NCBI
• RefSeq: WP_000608521 NCBI
• UniProt: see SACOL1480

⊟Protein sequence

• MINKEQLDLLYKLKKEVEKSRNEALLHTINQVIKKVYLQQYTCSFVGHFSAGKSTLINLLIEQDILPSSPVPTTSNTAIVSVSDNHDIIANLPNQTYAKLSNYDEVREMNRQNVDVESVEINFQSAKFENGFTLQDTPGVDSNVASHQSITEQYMYTSNMIFYTVDYNHVQSELNFKFMKHINDVGIPVVFIINQIDKHQDDELSFSTFKSRVEKSIADWGIKLERTFYVSKFDHPENELEVLSSYLISLDQHRETIEDYTSRTVEYITEAQLDYIQSEIQEVLEDLGIEEAEFEQAFLNSQQHQAISEEAQLLNNPDELMAFLKNKRKNILENAYIMPHNMREMLRSYLESMSQDFNVGGFFNKKKKKLQIQQQRLLTATDALQEHVNQQIRQPMREDMSFVTRFINKKEASDKVLNQHYDVKPEMIEGLYQPQTSISNTYVLTFSDEVVKAIKKYVEQQSTPIFKEIIENVQADELPTEESDDLKEYQRYTELNELRQSLTTKNYRHYYIHLDESLDKLIGRQETTYQVATDNAQDNRDNQQLNQNTATTNMSIDIQKALDIISDVPLFKRTKQDIHETLTRIDNKLIKIGVFGTFSAGKSSLINALLGEHILVSSPNPTTAATTEISYGDESYITLKSQSQLLDEINAVVEYQDMSYSTIEDFINSDLEKLKSHLNKNQLAFIQAVEKHYKLYVNMLENGEKHAINQQELKKWSAEDEYATFVKTVHIALMHDWLKGKIIVDSLGLHSNNQRHTNETEQILTSSDLILYVSYFNHSFTDNDKAFIEHMKDMNQLNENQAFKMVINAADLAESQDDLEAVETYVSDALRQVHLQSDIFAVSSRNALQAEDKGIDQLKQSIQQFVDVESKSILEQQMIHQLQQMDRSYVEMITEFETNKADISRRQQRLTDYKDKQRLQHQLIDATLQHTDNEVEEQVYHLNARLKLQLLDDVKSVFNSQMTQNSDFNEEKKVSTRVYLDQIHQRLFLEQSLITERIKKYFNKQLTEQIAPIVQQLADLHVIINPQFNFESANIEQPLLHIDFNDMLNALPKQLTKRKILNPNGQRDIHESICQSTLGLLQPQMGLLRQQLELYVKQMAVEAESQFESFEANIQTQINDLLAFDLDTTLINQLKDKHQQLKTILY

⊟Experimental data

• experimentally validated: see SACOL1480
• protein localization: see SACOL1480
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications