PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS08480 [old locus tag: SACOL1662 ]
• pan locus tag^?: SAUPAN004203000
• symbol: SACOL_RS08480
• pan gene symbol^?: —
• synonym:
• product: acetyl-CoA carboxylase biotin carboxyl carrier protein subunit

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS08480 [old locus tag: SACOL1662 ]
• symbol: SACOL_RS08480
• product: acetyl-CoA carboxylase biotin carboxyl carrier protein subunit
• replicon: chromosome
• strand: -
• coordinates: 1691374..1691823
• length: 450
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (1691374..1691823) NCBI
• BioCyc: SACOL_RS08480 BioCyc
• MicrobesOnline: see SACOL1662

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAATATTGAAAAAATCGAACAAATAATCAAATTAGTGAAGGAAAATGATGTTAAGAAA
  TTTAAATATAAAAATTTTGAAGATGAAATAGAAATTGACTTCACTGACTTGAATCATTTG
  GCTGCACACAGTAATCAATCAAATCAAAGTATGAACAATAATGATTTGACAGCTTCAAAA
  GCGAATGATAACTCCGATGTTTCGACAAATGATTATCATGACATTAAATCACCAATGATA
  GGTACATTCTTTTTACAAGATAGTAAAGAATTAACTGAACCAATTGTGAATGTCGGTGAC
  AAAGTTAACAAGGGAGATATTATAGGATATGTTGAAGCGATGAAAGTATTAAACGAGGTA
  ACAACAGATGTTGCTGGAGAAATTACTGAAATAGTAGCTGATCATGGCACAAATGTTGAA
  TACGACCAAGTTTTGGTACGTATTAAGTAA
  60
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⊟Protein

⊟General

• locus tag: SACOL_RS08480 [old locus tag: SACOL1662 ]
• symbol: SACOL_RS08480
• description: acetyl-CoA carboxylase biotin carboxyl carrier protein subunit
• length: 149
• theoretical pI: 4.33078
• theoretical MW: 16819.6
• GRAVY: -0.540268

⊟Function

• TIGRFAM:
  Metabolism Fatty acid and phospholipid metabolism Biosynthesis acetyl-CoA carboxylase, biotin carboxyl carrier protein (TIGR00531; HMM-score: 108.3)
  and 14 more
  Metabolism Central intermediary metabolism Nitrogen metabolism urea carboxylase (TIGR02712; EC 6.3.4.6; HMM-score: 35.1)
  Metabolism Transport and binding proteins Cations and iron carrying compounds oxaloacetate decarboxylase alpha subunit (TIGR01108; EC 4.1.1.3; HMM-score: 34)
  Metabolism Energy metabolism Other oxaloacetate decarboxylase alpha subunit (TIGR01108; EC 4.1.1.3; HMM-score: 34)
  Metabolism Energy metabolism Pyruvate dehydrogenase dihydrolipoyllysine-residue acetyltransferase (TIGR01348; EC 2.3.1.12; HMM-score: 25.4)
  Metabolism Energy metabolism Glycolysis/gluconeogenesis pyruvate carboxylase (TIGR01235; EC 6.4.1.1; HMM-score: 25.2)
  Metabolism Energy metabolism TCA cycle dihydrolipoyllysine-residue succinyltransferase, E2 component of oxoglutarate dehydrogenase (succinyl-transferring) complex (TIGR01347; EC 2.3.1.61; HMM-score: 23.6)
  2-oxoglutarate dehydrogenase, E2 component, dihydrolipoamide succinyltransferase (TIGR02927; EC 2.3.1.61; HMM-score: 18.5)
  Metabolism Energy metabolism ATP-proton motive force interconversion ATP synthase archaeal, A subunit (TIGR01043; EC 3.6.3.14; HMM-score: 17.3)
  selenium-dependent molybdenum hydroxylase system protein, YqeB family (TIGR03309; HMM-score: 16.7)
  Metabolism Energy metabolism Electron transport electron transport complex, RnfABCDGE type, C subunit (TIGR01945; HMM-score: 15.9)
  Metabolism Energy metabolism Amino acids and amines glycine cleavage system H protein (TIGR00527; HMM-score: 15.5)
  proline reductase-associated electron transfer protein PrdC (TIGR04481; HMM-score: 14.2)
  Metabolism Transport and binding proteins Unknown substrate efflux transporter, RND family, MFP subunit (TIGR01730; HMM-score: 12.6)
  Metabolism Energy metabolism Pyruvate dehydrogenase pyruvate dehydrogenase complex dihydrolipoamide acetyltransferase (TIGR01349; EC 2.3.1.12; HMM-score: 11.1)
• TheSEED: see SACOL1662
• PFAM:
  Hybrid (CL0105) Biotin_lipoyl; Biotin-requiring enzyme (PF00364; HMM-score: 67.3)
  and 7 more
  RnfC_N; RnfC Barrel sandwich hybrid domain (PF13375; HMM-score: 25.3)
  GCV_H; Glycine cleavage H-protein (PF01597; HMM-score: 21.1)
  Biotin_lipoyl_2; Biotin-lipoyl like (PF13533; HMM-score: 19.1)
  no clan defined ATP-synt_ab_Xtn; ATPsynthase alpha/beta subunit N-term extension (PF16886; HMM-score: 18.4)
  BET; Bromodomain extra-terminal - transcription regulation (PF17035; HMM-score: 16.1)
  Hybrid (CL0105) Peptidase_M23; Peptidase family M23 (PF01551; HMM-score: 13.8)
  no clan defined DUF1374; Protein of unknown function (DUF1374) (PF07118; HMM-score: 11.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.024442
  • TAT(Tat/SPI): 0.000791
  • LIPO(Sec/SPII): 0.001078
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446942078 NCBI
• RefSeq: WP_001019334 NCBI
• UniProt: see SACOL1662

⊟Protein sequence

• MNIEKIEQIIKLVKENDVKKFKYKNFEDEIEIDFTDLNHLAAHSNQSNQSMNNNDLTASKANDNSDVSTNDYHDIKSPMIGTFFLQDSKELTEPIVNVGDKVNKGDIIGYVEAMKVLNEVTTDVAGEITEIVADHGTNVEYDQVLVRIK

⊟Experimental data

• experimentally validated: see SACOL1662
• protein localization: see SACOL1662
• quantitative data / protein copy number per cell: see SACOL1662
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CcpA see SACOL1662

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications