From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS09780 [old locus tag: SACOL1899 ]
  • pan locus tag?: SAUPAN004773000
  • symbol: SACOL_RS09780
  • pan gene symbol?: —
  • synonym:
  • product: DNA double-strand break repair Rad50 ATPase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS09780 [old locus tag: SACOL1899 ]
  • symbol: SACOL_RS09780
  • product: DNA double-strand break repair Rad50 ATPase
  • replicon: chromosome
  • strand: -
  • coordinates: 1952932..1955868
  • length: 2937
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (1952932..1955868) NCBI
  • BioCyc: SACOL_RS09780 BioCyc
  • MicrobesOnline: see SACOL1899

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGATAATTAAATCACTTGAAATTTATGGTTACGGTCAATTTGTTCAACGTAAAATTGAA
    TTTAATAAAAACTTCACTGAAATTTTTGGTGAAAATGAAGCGGGTAAATCGACGATTCAA
    GCATTCATCCATTCGATATTATTTGGATTTCCAACTAAAAAGTCTAAAGAGCCAAGACTA
    GAACCACGTCTAGGTAACCAATACGGTGGTAAATTAGTACTTATTCTTGATGATGGCTTA
    GAGATTGAAGTTGAACGAATTAAAGGCAGTGCTCAAGGTGATGTGAAAGTATATTTACCT
    AATGGTGCTGTGCGTGATGATGCTTGGTTACAAAAGAAACTTAATTATATTTCTAAAAAG
    ACATATCAAGGTATCTTTTCATTTGATGTACTAGGGCTTCAAGACATTCATAGAAATCTA
    AATGAAAAACAATTGCAAGATTATTTATTACAAGCAGGGGCTTTAGGATCAACTGAATTC
    ACGTCAATGCGCGAAGTGATTAATCGTAAAAAAGATGAATTATATAAAAAATCAGGTAAA
    AATCCGATCATTAATCAACAAATTGAGCAATTAAAACAACTAGAAAGTCAAATTCGTGAA
    GAAGAAGCAAAGCTAGAAACATATCATCGCTTAGTAGATGATCGAGATAAATCATCACGT
    CGATTAGAGAATTTAAAGCATAATTTAAATCAATTATCAAAAATGCATGAAGAAAAACAA
    AAAGAGGTTGCTTTACATGATCATTCACAAGAATGGAAGTCTCTAGAACAACAGTTAAAT
    ATTGAGCCAATCACATTCCCAGAAAAAGGTGTGGATCGTTACGAAAAAGCACGAGCGCAT
    AAGCAATCGTTAGAAAGAGATATTGGTTTAAGAAATGAGCGTTTAGCTCAACTTAAAGAA
    GAAGCGACTCAATTAGAGCCAGTTAAACAATCTGATATTGACGCCTTCATTAGTTTGAAT
    CAACAAGAAAATGAAATTAAAAATAAAGAATTTGAACTTACTGCAATCGAAAAGGATATT
    GCGAATAAACAACGTGATAAAGATGAATTGCAATCAAATATTGGTTGGTCTGAAACGCAT
    CATGACGTAGATAGTTCAGAGGCAATGAAAAGTTATGTCAGTGAGCAAATCAAGAATAAA
    CAAGAACAAGCTGCATACATTAAACAATTAGAACGTAGTTTAGAAGAAAATAAAATCGAA
    GATAATGCGGTTCATAGCGAACTAGATTCTGTTGAAGAAAAAATAGTTCCTGAAGAGACT
    TTTGAAAAGAAAAAAGAATACTCACAACAAGTCATTGAATTAAACGAAAAAGAGAACTTG
    TATAGCAAATTGAAAGAACGTTTCGAAATTGAGCAACAAGAAAAACAAAAACGTCAAAAA
    TTGTTACGAACAACGTTTATTCTTTTAACATTAGTAGGTATTGGTTTAACTGCCTTCTCA
    TTTATTTCAAACAACATGTTGTTTGGTATTATTTTCGCAGTATTAACTTTAGTATTTGTT
    ATCGGTATCATCATGTCTAAATCAAAAGAAGTAGATTATAGCGAGGCCATCACAGATGAA
    ATCGAAGAAATTAAAGCACAACTAGCCATTTTAGATGAAAATTATGACTTAGATTTTGAT
    CTTGATGAGCAATATCGCATTCGTGATCACTGGCAACAAGCTTTGAAAAATAAAGATATT
    TTAGAAGAGAAACGTCAGTATATTGAAGGTCGTTTAAATGATGCGAAAGGGCGTCATGAT
    GAATTACAATCGACAGTTGAGAACGTTAAGGATGAATTATACTTATCATCTAAAATTTCA
    AATGATTTAATTGTTGATAGTATTTCTACAATGGCGAATATTAAAGCATTAGATCAACAT
    ATCAGTGATTTAAATCAACAACGCCAGCAATTGGTTCAAGAATTAGATACATTCTACAAT
    CATGCTGAAGCTGTTACAAAATCACAATTTGTTTATTTCAACAAATTATCTTTATTCCAC
    GATGTGCAACAATGGTTGAAATCAGCTGAAGACACGAATGAAAAATGGCGTATTAATGCT
    GAAAATACCAAACTTGTTACAAATGAATTAAATCATTTAAATGCTCAATTAGAAGAAAAT
    AATAAAGAAATTACAGCACTATTCGATTTTATCAATGTTGGTACTGAAGAAGATTTTTAC
    CAACATCATGAAGATTACCAAACTTATACTAGTAATTTGAGTCGTTTTAATGATTTAACT
    AAATATCTTGAGAACCAAAACTATTCTTATGAATTAAGTTCTAGTTTAAGTGAAAAAACA
    ACTGCACAACTTGAAGAAGAAGATCATTTATTGGCTACTCAAGTTGACGAATATAATGAG
    CAATATCTTGAAATGCAAGCACAAGTCAGTGATTTAAGTGCACAAATCAATCACATGGAA
    ACTGATACAACGCTTGCTAATTTAAGACATGAATATCACAGTCTTAAAAATCAACTTAAT
    GATATCGCAAAAGATTGGGCAAGTTTAAGTTATTTACAAAGTTTAGTTGATGAACACATT
    AAACAAATTAAAGATAAACGTTTGCCTCAAGTTATTAATGAAGCGGTAGAAATATTGAAG
    CATTTAACAGATGGCAGATATACGATGATTAACTATAATGAAGATTCAATTACGGTTAAA
    CATGTTAATGGTCAATTATATGATCCTGTTGAACTAAGTCAATCTACAAAAGAATTACTT
    TATGTAGCTTTACGTATCAGTTTAATTAAGGTACTAAGACCATATTATCCGTTCCCATTA
    ATTGTTGATGATGCATTTGTTCATTTTGATAAAAAACGTACTGAAAAAATGTTGAATTAT
    TTACGATCATTATCAGAACACTATCAAGTACTATACTTTACATGTGTAAAAGATAATATT
    GTTCCATCAAAAGAAGTGATTACATTAAACAAAATAGAGGAAGGCGGGAAACGATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2700
    2760
    2820
    2880
    2937

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL_RS09780 [old locus tag: SACOL1899 ]
  • symbol: SACOL_RS09780
  • description: DNA double-strand break repair Rad50 ATPase
  • length: 978
  • theoretical pI: 4.97145
  • theoretical MW: 114420
  • GRAVY: -0.726278

⊟Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Cell division chromosome segregation protein SMC (TIGR02169; HMM-score: 17.7)
    Genetic information processing DNA metabolism Chromosome-associated proteins chromosome segregation protein SMC (TIGR02169; HMM-score: 17.7)
    Cellular processes Cellular processes Cell division chromosome segregation protein SMC (TIGR02168; HMM-score: 17.6)
    Genetic information processing DNA metabolism Chromosome-associated proteins chromosome segregation protein SMC (TIGR02168; HMM-score: 17.6)
  • TheSEED: see SACOL1899
  • PFAM:
    P-loop_NTPase (CL0023) AAA_27; AAA domain (PF13514; HMM-score: 85.8)
    and 11 more
    AAA_23; AAA domain (PF13476; HMM-score: 48)
    no clan defined CENP-F_leu_zip; Leucine-rich repeats of kinetochore protein Cenp-F/LEK1 (PF10473; HMM-score: 26.1)
    Hypoth_1 (CL0447) DUF3784; Domain of unknown function (DUF3784) (PF12650; HMM-score: 19.7)
    P-loop_NTPase (CL0023) AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 19.7)
    SMC_N; RecF/RecN/SMC N terminal domain (PF02463; HMM-score: 16.6)
    no clan defined DUF4191; Domain of unknown function (DUF4191) (PF13829; HMM-score: 16)
    P-loop_NTPase (CL0023) SbcCD_C; Putative exonuclease SbcCD, C subunit (PF13558; HMM-score: 14.3)
    GPCR_A (CL0192) 7tm_2; 7 transmembrane receptor (Secretin family) (PF00002; HMM-score: 13.8)
    no clan defined EcsB; Bacterial ABC transporter protein EcsB (PF05975; HMM-score: 11)
    FtsH_ext; FtsH Extracellular (PF06480; HMM-score: 9.8)
    DALR (CL0258) DALR_1; DALR anticodon binding domain (PF05746; HMM-score: 9.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 2
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006246
    • TAT(Tat/SPI): 0.000529
    • LIPO(Sec/SPII): 0.001162
  • predicted transmembrane helices (TMHMM): 2

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIIKSLEIYGYGQFVQRKIEFNKNFTEIFGENEAGKSTIQAFIHSILFGFPTKKSKEPRLEPRLGNQYGGKLVLILDDGLEIEVERIKGSAQGDVKVYLPNGAVRDDAWLQKKLNYISKKTYQGIFSFDVLGLQDIHRNLNEKQLQDYLLQAGALGSTEFTSMREVINRKKDELYKKSGKNPIINQQIEQLKQLESQIREEEAKLETYHRLVDDRDKSSRRLENLKHNLNQLSKMHEEKQKEVALHDHSQEWKSLEQQLNIEPITFPEKGVDRYEKARAHKQSLERDIGLRNERLAQLKEEATQLEPVKQSDIDAFISLNQQENEIKNKEFELTAIEKDIANKQRDKDELQSNIGWSETHHDVDSSEAMKSYVSEQIKNKQEQAAYIKQLERSLEENKIEDNAVHSELDSVEEKIVPEETFEKKKEYSQQVIELNEKENLYSKLKERFEIEQQEKQKRQKLLRTTFILLTLVGIGLTAFSFISNNMLFGIIFAVLTLVFVIGIIMSKSKEVDYSEAITDEIEEIKAQLAILDENYDLDFDLDEQYRIRDHWQQALKNKDILEEKRQYIEGRLNDAKGRHDELQSTVENVKDELYLSSKISNDLIVDSISTMANIKALDQHISDLNQQRQQLVQELDTFYNHAEAVTKSQFVYFNKLSLFHDVQQWLKSAEDTNEKWRINAENTKLVTNELNHLNAQLEENNKEITALFDFINVGTEEDFYQHHEDYQTYTSNLSRFNDLTKYLENQNYSYELSSSLSEKTTAQLEEEDHLLATQVDEYNEQYLEMQAQVSDLSAQINHMETDTTLANLRHEYHSLKNQLNDIAKDWASLSYLQSLVDEHIKQIKDKRLPQVINEAVEILKHLTDGRYTMINYNEDSITVKHVNGQLYDPVELSQSTKELLYVALRISLIKVLRPYYPFPLIVDDAFVHFDKKRTEKMLNYLRSLSEHYQVLYFTCVKDNIVPSKEVITLNKIEEGGKR

⊟Experimental data[edit | edit source]

  • experimentally validated: see SACOL1899
  • protein localization: see SACOL1899
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SACOL_RS09780&oldid=89050"