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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL_RS10330 [old locus tag: SACOL1976 ]
- pan locus tag?: SAUPAN004947000
- symbol: SACOL_RS10330
- pan gene symbol?: nos
- synonym:
- product: nitric oxide synthase oxygenase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL_RS10330 [old locus tag: SACOL1976 ]
- symbol: SACOL_RS10330
- product: nitric oxide synthase oxygenase
- replicon: chromosome
- strand: +
- coordinates: 2039628..2040704
- length: 1077
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
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1021ATGTTATTTAAAGAGGCTCAAGCTTTCATAGAAAACATGTATAAAGAGTGTCATTATGAA
ACGCAAATTATCAATAAACGTTTACATGACATTGAACTAGAAATAAAAGAAACTGGGACA
TATACACATACAGAAGAAGAACTTATTTATGGTGCTAAAATGGCTTGGCGTAATTCAAAT
CGTTGCATTGGTCGTTTATTTTGGGATTCGTTAAATGTCATTGATGCAAGAGATGTTACT
GACGAAGCATCGTTCTTATCATCAATTACTTATCATATTACACAGGCTACAAATGAAGGT
AAATTAAAGCCGTATATTACTATATATGCTCCAAAGGATGGACCTAAAATTTTCAACAAT
CAATTAATTCGCTATGCTGGCTATGACAATTGTGGTGATCCTGCTGAAAAAGAAGTTACA
CGCTTAGCAAATCACTTAGGTTGGAAAGGAAAAGGTACTAATTTTGACGTGTTACCACTG
ATTTACCAATTACCTAATGAGTCAGTTAAATTTTACGAATATCCTACTTCATTAATTAAA
GAAGTACCTATTGAACATAATCATTATCCAAAATTAAGAAAATTGAACTTAAAATGGTAT
GCAGTCCCTATCATTTCCAATATGGACTTAAAAATCGGTGGCATTGTATATCCAACTGCA
CCCTTTAACGGTTGGTATATGGTAACTGAAATTGGCGTACGTAACTTTATTGATGATTAC
CGTTACAATTTACTAGAAAAAGTTGCAGATGCGTTTGAATTTGATACACTTAAAAATAAT
TCATTTAATAAAGATCGAGCACTTGTTGAATTGAACTATGCTGTGTATCATTCCTTTAAA
AAAGAAGGCGTATCAATTGTCGATCATTTGACCGCTGCAAAGCAATTCGAACTATTCGAA
CGTAACGAAGCACAACAAGGTCGTCAAGTTACCGGAAAATGGTCTTGGCTAGCACCGCCA
TTATCTCCAACATTGACGTCAAATTATCATCACGGATATGACAATACAGTAAAAGATCCA
AACTTTTTCTATAAAAAGAAAGAATCAAATGCTAACCAGTGCCCTTTCCATCATTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL_RS10330 [old locus tag: SACOL1976 ]
- symbol: SACOL_RS10330
- description: nitric oxide synthase oxygenase
- length: 358
- theoretical pI: 7.00032
- theoretical MW: 41709.9
- GRAVY: -0.563966
⊟Function[edit | edit source]
- reaction: EC 1.14.14.47? ExPASyNitric-oxide synthase (flavodoxin) 2 L-arginine + 3 reduced flavodoxin + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 oxidized flavodoxin + 4 H2O
- TIGRFAM:
- TheSEED: see SACOL1976
- PFAM: no clan defined NO_synthase; Nitric oxide synthase, oxygenase domain (PF02898; HMM-score: 506)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors: (6S)-5,6,7,8-tetrahydrofolate, heme
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.004813
- TAT(Tat/SPI): 0.000432
- LIPO(Sec/SPII): 0.000773
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MLFKEAQAFIENMYKECHYETQIINKRLHDIELEIKETGTYTHTEEELIYGAKMAWRNSNRCIGRLFWDSLNVIDARDVTDEASFLSSITYHITQATNEGKLKPYITIYAPKDGPKIFNNQLIRYAGYDNCGDPAEKEVTRLANHLGWKGKGTNFDVLPLIYQLPNESVKFYEYPTSLIKEVPIEHNHYPKLRKLNLKWYAVPIISNMDLKIGGIVYPTAPFNGWYMVTEIGVRNFIDDYRYNLLEKVADAFEFDTLKNNSFNKDRALVELNYAVYHSFKKEGVSIVDHLTAAKQFELFERNEAQQGRQVTGKWSWLAPPLSPTLTSNYHHGYDNTVKDPNFFYKKKESNANQCPFHH
⊟Experimental data[edit | edit source]
- experimentally validated: see SACOL1976
- protein localization: see SACOL1976
- quantitative data / protein copy number per cell: see SACOL1976
- interaction partners:
SACOL_RS01040 formate acetyltransferase [1] (data from MRSA252) SACOL_RS03030 50S ribosomal protein L1 [1] (data from MRSA252) SACOL_RS03075 elongation factor G [1] (data from MRSA252) SACOL_RS03080 elongation factor Tu [1] (data from MRSA252) SACOL_RS04330 enolase [1] (data from MRSA252) SACOL_RS06365 beta-ketoacyl-ACP reductase [1] (data from MRSA252) SACOL_RS06575 translation initiation factor IF-2 [1] (data from MRSA252) SACOL_RS08905 isocitrate dehydrogenase (NADP(+)) [1] (data from MRSA252) SACOL_RS08930 pyruvate kinase [1] (data from MRSA252) SACOL_RS11010 uracil phosphoribosyltransferase [1] (data from MRSA252) SACOL_RS11615 30S ribosomal protein S9 [1] (data from MRSA252) SACOL_RS11705 50S ribosomal protein L6 [1] (data from MRSA252) SACOL_RS11775 50S ribosomal protein L4 [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)