From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS10470 [old locus tag: SACOL2003 ]
  • pan locus tag?: SAUPAN005012000
  • symbol: SACOL_RS10470
  • pan gene symbol?: hlb-2
  • synonym:
  • product: phosphodiesterase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS10470 [old locus tag: SACOL2003 ]
  • symbol: SACOL_RS10470
  • product: phosphodiesterase
  • replicon: chromosome
  • strand: +
  • coordinates: 2063726..2064718
  • length: 993
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (2063726..2064718) NCBI
  • BioCyc: SACOL_RS10470 BioCyc
  • MicrobesOnline: see SACOL2003

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGGTGAAAAAAACAAAATCCAATTCACTAAAAAAAGTTGCAACACTTGCATTAGCAAAT
    TTATTATTAGTTGGTGCACTTACTGACAATAGTGCCAAAGCCGAATCTAAGAAAGATGAT
    ACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAAC
    TGGGGGCAATATAAACGCGCTGATTTAATCGGACAATCTTCTTATATTAAAAATAATGAT
    GTCGTAATATTCAATGAAGCATTTGATAATGGTGCATCAGACAAATTATTAAGTAATGTG
    AAAAAAGAATATCCTTATCAAACACCTGTACTCGGCCGTTCTCAATCAGGGTGGGACAAA
    ACTGAAGGTAGCTACTCATCAACTGTTGCAGAAGATGGTGGCGTAGCGATTGTAAGTAAA
    TATCCTATTAAAGAAAAAATCCAGCATGTTTTCAAAAGCGGTTGTGGATTCGATAATGAT
    AGCAACAAAGGCTTTGTTTATACAAAAATAGAGAAAAATGGTAAGAACGTTCACGTTATC
    GGTACACATACACAATCTGAAGATTCACGTTGTGGTGCTGGACATGATCGAAAAATTAGA
    GCTGAACAAATGAAAGAAATCAGTGACTTTGTTAAAAAGAAAAATATCCCTAAAGATGAA
    ACGGTATATATAGGTGGCGACCTTAATGTCAATAAAGGCACTCCAGAGTTCAAAGATATG
    CTTAAAAACTTGAATGTAAATGATGTTCTATATGCAGGTCATAATAGCACATGGGACCCT
    CAATCAAATTCAATTGCGAAATATAATTACCCTAATGGTAAACCAGAACATTTAGACTAT
    ATATTTACAGATAAAGATCATAAACAACCAAAACAATTAGTCAATGAAGTTGTGACTGAA
    AAACCTAAGCCATGGGATGTATATGCGTTCCCATATTACTACGTTTACAATGATTTTTCA
    GATCATTACCCAATCAAAGCCTATAGTAAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    993

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL_RS10470 [old locus tag: SACOL2003 ]
  • symbol: SACOL_RS10470
  • description: phosphodiesterase
  • length: 330
  • theoretical pI: 9.09301
  • theoretical MW: 37237.6
  • GRAVY: -0.734848

⊟Function[edit | edit source]

  • reaction:
    EC 3.1.4.3?  ExPASy
    Phospholipase C A phosphatidylcholine + H2O = 1,2-diacyl-sn-glycerol + phosphocholine
  • TIGRFAM:
    Cellular processes Cellular processes Pathogenesis sphingomyelin phosphodiesterase (TIGR03395; EC 3.1.4.12; HMM-score: 405)
  • TheSEED: see SACOL2003
  • PFAM:
    DNase_I-like (CL0530) Exo_endo_phos; Endonuclease/Exonuclease/phosphatase family (PF03372; HMM-score: 53.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 10
    • Internal Helices: 0
  • LocateP:
  • SignalP: Signal peptide SP(Sec/SPI) length 34 aa
    • SP(Sec/SPI): 0.971402
    • TAT(Tat/SPI): 0.007361
    • LIPO(Sec/SPII): 0.016029
    • Cleavage Site: CS pos: 34-35. AKA-ES. Pr: 0.9247
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MVKKTKSNSLKKVATLALANLLLVGALTDNSAKAESKKDDTDLKLVSHNVYMLSTVLYPNWGQYKRADLIGQSSYIKNNDVVIFNEAFDNGASDKLLSNVKKEYPYQTPVLGRSQSGWDKTEGSYSSTVAEDGGVAIVSKYPIKEKIQHVFKSGCGFDNDSNKGFVYTKIEKNGKNVHVIGTHTQSEDSRCGAGHDRKIRAEQMKEISDFVKKKNIPKDETVYIGGDLNVNKGTPEFKDMLKNLNVNDVLYAGHNSTWDPQSNSIAKYNYPNGKPEHLDYIFTDKDHKQPKQLVNEVVTEKPKPWDVYAFPYYYVYNDFSDHYPIKAYSK

⊟Experimental data[edit | edit source]

  • experimentally validated: see SACOL2003
  • protein localization: see SACOL2003
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SACOL_RS10470&oldid=80938"