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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL_RS10605 [old locus tag: SACOL2031 ]
- pan locus tag?: SAUPAN005287000
- symbol: SACOL_RS10605
- pan gene symbol?: nrgA
- synonym:
- product: ammonia permease
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL_RS10605 [old locus tag: SACOL2031 ]
- symbol: SACOL_RS10605
- product: ammonia permease
- replicon: chromosome
- strand: -
- coordinates: 2089985..2091235
- length: 1251
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
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1201ATGAATCTTAACGATACGATATTTATGTTTTTGTGTACATTATTAGTTTGGTTAATGACA
CCAGGATTAAGTTTATTTTATGGTGGGTTAGTTGAATCTAAAAATGCGCTTAATACTGTC
ATGCAAAGTATGGCAGCAATTGTGCTTGTTACATTTGTATGGATAACAGTTGGTTTTACA
ATTAGTTTTGGGAATGGGAATTTATGGTTCGGAAATTGGGAATATACTTTTCTTAATCAT
GTAGGTTTTGCGACTCAAGAAGATATTAGCCCACATATTCCTTTCGCTTTGTTTATGTTA
TTTCAAATGATGTTTTGTACGATTGCAATTTCTATTTTATCTGGTTCAATCGCTGAGAAA
ATGAAGTTTATTCCTTATTTATTATTCGTAGTAATATGGACTGCTCTTGTATACAGTCCA
GTAGCACATTGGGTTTGGGGCGGCGGTTGGATTAACAAACTCGGTGTATTAGATTTCGCT
GGAGGTACGGTTGTTCATATTACATCAGGTGTTTCTGGTTTAGTATTAGCTATTATGATT
GGAAAAGGAAACAAACATTCTGAATCAACACCACATAATCTTATCATTACGTTGATTGGC
GGTATATTCGTGTGGATTGGTTGGTATGGATTTAATGTAGGTAGTGCTTTTACATTTGAT
AATATTGCGATGCTTGCATTTACAAATACTGTCATTTCAGCCAGTGCAGGTGCTATAGGT
TGGTTAATTTTAGAATATATTTTTAAAAAGACGACAAGTTTACTTGGACTTTTACTCGGT
GCATTAGCAGGATTAGTTGTCATTACTCCTGCAGCAGGATATGTAACATATCTTAGTGCA
ACAATAATGGCTTTAATAGGAGGTATCTGTTGTTATATTGTCATTAATTACATCAAGGTA
AAACTAAAATATCATGATGCATTAGATGCATTTGGTATTCATGGTGTTGGTGGTATTATT
GGTGCTGTTTTAACAGCAGTTTTCCAAAGTAAAAAAGCCAATCCTGACATTGAGAATGGC
TTTATTTATACTGGTGACATACATATTATACTTGTACAAATATTATGTGTAACAGCAGTT
GTAATTTTTAGTATAGTCATGACGTTTATTATTGCGAAAGTAATTAAATTAATTACACCA
TTATCTGTTACGGAACAAGAAACGAATATAGGATTAGACAAGATTGTTCACGGTGAACAT
GCTTACTTTGAAGGTGAGCTAAATAGATTCAATAAACATATTCGATATTAG60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL_RS10605 [old locus tag: SACOL2031 ]
- symbol: SACOL_RS10605
- description: ammonia permease
- length: 416
- theoretical pI: 7.13818
- theoretical MW: 45479.6
- GRAVY: 0.852885
⊟Function[edit | edit source]
- TIGRFAM: Transport and binding proteins Cations and iron carrying compounds ammonium transporter (TIGR00836; HMM-score: 404.9)and 1 moreprobable ammonium transporter, marine subtype (TIGR03644; HMM-score: 196.3)
- TheSEED: see SACOL2031
- PFAM: no clan defined Ammonium_transp; Ammonium Transporter Family (PF00909; HMM-score: 376.2)and 1 moreGlycine-zipper (CL0500) Gly-zipper_YMGG; YMGG-like Gly-zipper (PF13441; HMM-score: 7)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 11
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.024099
- TAT(Tat/SPI): 0.000837
- LIPO(Sec/SPII): 0.028064
- predicted transmembrane helices (TMHMM): 11
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MNLNDTIFMFLCTLLVWLMTPGLSLFYGGLVESKNALNTVMQSMAAIVLVTFVWITVGFTISFGNGNLWFGNWEYTFLNHVGFATQEDISPHIPFALFMLFQMMFCTIAISILSGSIAEKMKFIPYLLFVVIWTALVYSPVAHWVWGGGWINKLGVLDFAGGTVVHITSGVSGLVLAIMIGKGNKHSESTPHNLIITLIGGIFVWIGWYGFNVGSAFTFDNIAMLAFTNTVISASAGAIGWLILEYIFKKTTSLLGLLLGALAGLVVITPAAGYVTYLSATIMALIGGICCYIVINYIKVKLKYHDALDAFGIHGVGGIIGAVLTAVFQSKKANPDIENGFIYTGDIHIILVQILCVTAVVIFSIVMTFIIAKVIKLITPLSVTEQETNIGLDKIVHGEHAYFEGELNRFNKHIRY
⊟Experimental data[edit | edit source]
- experimentally validated:
- protein localization:
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: CodY, GlnR see SACOL2031
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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