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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS10695 [old locus tag: SACOL2048 ]
  • pan locus tag?: SAUPAN005309000
  • symbol: SACOL_RS10695
  • pan gene symbol?: leuC
  • synonym:
  • product: 3-isopropylmalate dehydratase large subunit

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS10695 [old locus tag: SACOL2048 ]
  • symbol: SACOL_RS10695
  • product: 3-isopropylmalate dehydratase large subunit
  • replicon: chromosome
  • strand: +
  • coordinates: 2108571..2109941
  • length: 1371
  • essential: unknown

Accession numbers[edit | edit source]

  • Location: NC_002951 (2108571..2109941) NCBI
  • BioCyc: G1G4B-2249 BioCyc
  • MicrobesOnline:

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGGTCAAACATTATTTGACAAGGTGTGGAACAGACATGTGTTATACGGGAAATTGGGC
    GAACCGCAACTATTATACATTGATTTACACCTTATACATGAAGTTACTTCTCCTCAAGCA
    TTTGAAGGACTTAGGCTTCAAAACAGAAAATTAAGACGCCCAGATTTAACATTTGCAACA
    CTCGATCACAATGTTCCTACTATTGATATATTCAATATTAAAGATGAAATTGCAAACAAA
    CAAATCACAACATTACAAAAAAACGCCATAGATTTTGGGGTGCATATTTTTGATATGGGT
    TCTGATGAACAAGGTATTGTTCACATGGTAGGACCTGAGACAGGACTTACACAGCCTGGC
    AAGACAATCGTTTGTGGTGACTCTCACACAGCAACACATGGAGCATTTGGTGCTATTGCA
    TTTGGAATTGGAACAAGTGAAGTTGAACATGTTTTCGCAACTCAAACGCTATGGCAAACA
    AAACCCAAAAACTTAAAAATCGATATTAATGGTACCTTACCAACAGGCGTCTATGCTAAG
    GACATTATTCTGCATTTAATTAAAACGTATGGTGTTGACTTTGGTACAGGCTATGCTTTG
    GAATTTACTGGCGAAACAATTAAAAACCTTTCAATGGATGGTCGAATGACTATTTGTAAC
    ATGGCTATCGAAGGTGGTGCCAAATACGGCATAATCCAACCTGATGATATAACATTTGAA
    TATGTTAAAGGGAGACCATTTGCCGATAACTTCGCTAAATCAGTTGATAAGTGGCGTGAG
    CTATATTCTGATGACGACGCGATATTTGATCGTGTAATTGAACTTGATGTTTCAACATTA
    GAACCACAAGTGACATGGGGAACTAATCCTGAAATGGGTGTTAATTTCAGTGAACCATTC
    CCTGAAATCAATGATATCAACGATCAACGTGCGTATGATTATATGGGGTTAGAACCAGGT
    CAAAAAGCTGAAGACATCGACTTAGGGTATGTTTTTCTCGGTTCATGTACAAATGCTAGA
    CTATCAGATTTGATTGAAGCTAGTCATATTGTTAAAGGAAATAAAGTTCATCCAAATATT
    ACAGCTATTGTCGTACCAGGTTCTCGTACAGTAAAAAAAGAAGCAGAAAAATTAGGTCTA
    GATACTATCTTTAAAAATGCAGGATTTGAATGGCGTGAACCAGGATGTTCAATGTGTTTA
    GGCATGAATCCTGACCAAGTACCTGAGGGCGTACATTGTGCATCTACAAGTAATCGAAAC
    TTTGAAGGACGACAAGGCAAAGGTGCAAGAACACATTTAGTATCCCCTGCTATGGCAGCA
    GCAGCAGCTATTCATGGTAAATTTGTGGACGTAAGAAAGGTGGTTGTTTAA
    60
    120
    180
    240
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    360
    420
    480
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    960
    1020
    1080
    1140
    1200
    1260
    1320
    1371

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL_RS10695 [old locus tag: SACOL2048 ]
  • symbol: SACOL_RS10695
  • description: 3-isopropylmalate dehydratase large subunit
  • length: 456
  • theoretical pI: 5.56699
  • theoretical MW: 50325.9
  • GRAVY: -0.230263

Function[edit | edit source]

  • reaction:
    EC 4.2.1.33?  ExPASy
    3-isopropylmalate dehydratase (2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Pyruvate family 3-isopropylmalate dehydratase, large subunit (TIGR00170; EC 4.2.1.33; HMM-score: 695.6)
    and 9 more
    homoaconitate hydratase family protein (TIGR01343; HMM-score: 340.7)
    3-isopropylmalate dehydratase, large subunit (TIGR02086; HMM-score: 305.7)
    Metabolism Amino acid biosynthesis Pyruvate family 3-isopropylmalate dehydratase, large subunit (TIGR02083; EC 4.2.1.33; HMM-score: 287.2)
    Metabolism Energy metabolism TCA cycle putative aconitate hydratase (TIGR01342; EC 4.2.1.3; HMM-score: 206.5)
    Metabolism Amino acid biosynthesis Aspartate family homoaconitase (TIGR00139; EC 4.2.1.36; HMM-score: 151.5)
    Metabolism Energy metabolism TCA cycle aconitate hydratase, mitochondrial (TIGR01340; EC 4.2.1.3; HMM-score: 144.5)
    Metabolism Energy metabolism TCA cycle aconitate hydratase 1 (TIGR01341; EC 4.2.1.3; HMM-score: 105.1)
    2-methylisocitrate dehydratase, Fe/S-dependent (TIGR02333; EC 4.2.1.99; HMM-score: 90.4)
    Metabolism Energy metabolism TCA cycle aconitate hydratase 2 (TIGR00117; EC 4.2.1.3; HMM-score: 71.6)
  • TheSEED:
  • PFAM:
    no clan defined Aconitase; Aconitase family (aconitate hydratase) (PF00330; HMM-score: 491.4)
    and 1 more
    DUF521; Protein of unknown function (DUF521) (PF04412; HMM-score: 13.8)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors: [4Fe-4S] cluster
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001455
    • TAT(Tat/SPI): 0.000137
    • LIPO(Sec/SPII): 0.000258
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

  • GI: 446453968 NCBI
  • RefSeq: WP_000531823 NCBI
  • UniProt:

Protein sequence[edit | edit source]

  • MGQTLFDKVWNRHVLYGKLGEPQLLYIDLHLIHEVTSPQAFEGLRLQNRKLRRPDLTFATLDHNVPTIDIFNIKDEIANKQITTLQKNAIDFGVHIFDMGSDEQGIVHMVGPETGLTQPGKTIVCGDSHTATHGAFGAIAFGIGTSEVEHVFATQTLWQTKPKNLKIDINGTLPTGVYAKDIILHLIKTYGVDFGTGYALEFTGETIKNLSMDGRMTICNMAIEGGAKYGIIQPDDITFEYVKGRPFADNFAKSVDKWRELYSDDDAIFDRVIELDVSTLEPQVTWGTNPEMGVNFSEPFPEINDINDQRAYDYMGLEPGQKAEDIDLGYVFLGSCTNARLSDLIEASHIVKGNKVHPNITAIVVPGSRTVKKEAEKLGLDTIFKNAGFEWREPGCSMCLGMNPDQVPEGVHCASTSNRNFEGRQGKGARTHLVSPAMAAAAAIHGKFVDVRKVVV

Experimental data[edit | edit source]

  • experimentally validated: no data available

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]