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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02287
  • pan locus tag?: SAUPAN005309000
  • symbol: SAOUHSC_02287
  • pan gene symbol?: leuC
  • synonym:
  • product: isopropylmalate isomerase large subunit

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02287
  • symbol: SAOUHSC_02287
  • product: isopropylmalate isomerase large subunit
  • replicon: chromosome
  • strand: +
  • coordinates: 2119308..2120678
  • length: 1371
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGGTCAAACATTATTTGACAAGGTGTGGAACAGACATGTGTTATACGGGAAATTGGGC
    GAACCGCAACTATTATACATTGATTTACACCTTATACATGAAGTTACTTCTCCTCAAGCA
    TTTGAAGGACTTAGGCTTCAAAACAGAAAATTAAGACGCCCAGATTTAACATTTGCAACA
    CTCGATCACAATGTTCCTACTATTGATATATTCAATATTAAAGATGAAATTGCAAACAAA
    CAAATCACAACATTACAAAAAAACGCCATAGATTTTGGGGTGCATATTTTTGATATGGGT
    TCTGATGAACAAGGTATTGTTCACATGGTAGGACCTGAGACAGGACTTACACAGCCTGGC
    AAGACAATCGTTTGTGGTGACTCTCACACAGCAACACATGGAGCATTTGGTGCTATTGCA
    TTTGGAATTGGAACAAGTGAAGTTGAACATGTTTTCGCAACTCAAACGCTATGGCAAACA
    AAACCCAAAAACTTAAAAATCGATATTAATGGTACCTTACCAACAGGCGTCTATGCTAAG
    GACATTATTCTGCATTTAATTAAAACGTATGGTGTTGACTTTGGTACAGGCTATGCTTTG
    GAATTTACTGGCGAAACAATTAAAAACCTTTCAATGGATGGTCGAATGACTATTTGTAAC
    ATGGCTATCGAAGGTGGTGCCAAATACGGCATAATCCAACCTGATGATATAACATTTGAA
    TATGTTAAAGGGAGACCATTTGCCGATAACTTCGCTAAATCAGTTGATAAGTGGCGTGAG
    CTATATTCTGATGACGACGCGATATTTGATCGTGTAATTGAACTTGATGTTTCAACATTA
    GAACCACAAGTGACATGGGGAACTAATCCTGAAATGGGTGTTAATTTCAGTGAACCATTC
    CCTGAAATCAATGATATCAACGATCAACGTGCGTATGATTATATGGGGTTAGAACCAGGT
    CAAAAAGCTGAAGACATCGACTTAGGGTATGTTTTTCTCGGTTCATGTACAAATGCTAGA
    CTATCAGATTTGATTGAAGCTAGTCATATTGTTAAAGGAAATAAAGTTCATCCAAATATT
    ACAGCTATTGTCGTACCAGGTTCTCGTACAGTAAAAAAAGAAGCAGAAAAATTAGGTCTA
    GATACTATCTTTAAAAATGCAGGATTTGAATGGCGTGAACCAGGATGTTCAATGTGTTTA
    GGCATGAATCCTGACCAAGTACCTGAGGGCGTACATTGTGCATCTACAAGTAATCGAAAC
    TTTGAAGGACGACAAGGCAAAGGTGCAAGAACACATTTAGTATCCCCTGCTATGGCAGCA
    GCAGCAGCTATTCATGGTAAATTTGTGGACGTAAGAAAGGTGGTTGTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1371

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02287
  • symbol: SAOUHSC_02287
  • description: isopropylmalate isomerase large subunit
  • length: 456
  • theoretical pI: 5.56699
  • theoretical MW: 50325.9
  • GRAVY: -0.230263

⊟Function[edit | edit source]

  • reaction:
    EC 4.2.1.33?  ExPASy
    3-isopropylmalate dehydratase (2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Pyruvate family 3-isopropylmalate dehydratase, large subunit (TIGR00170; EC 4.2.1.33; HMM-score: 695.6)
    and 9 more
    homoaconitate hydratase family protein (TIGR01343; HMM-score: 340.7)
    3-isopropylmalate dehydratase, large subunit (TIGR02086; HMM-score: 305.7)
    Metabolism Amino acid biosynthesis Pyruvate family 3-isopropylmalate dehydratase, large subunit (TIGR02083; EC 4.2.1.33; HMM-score: 287.2)
    Metabolism Energy metabolism TCA cycle putative aconitate hydratase (TIGR01342; EC 4.2.1.3; HMM-score: 206.5)
    Metabolism Amino acid biosynthesis Aspartate family homoaconitase (TIGR00139; EC 4.2.1.36; HMM-score: 151.5)
    Metabolism Energy metabolism TCA cycle aconitate hydratase, mitochondrial (TIGR01340; EC 4.2.1.3; HMM-score: 144.5)
    Metabolism Energy metabolism TCA cycle aconitate hydratase 1 (TIGR01341; EC 4.2.1.3; HMM-score: 105.1)
    2-methylisocitrate dehydratase, Fe/S-dependent (TIGR02333; EC 4.2.1.99; HMM-score: 90.4)
    Metabolism Energy metabolism TCA cycle aconitate hydratase 2 (TIGR00117; EC 4.2.1.3; HMM-score: 71.6)
  • TheSEED  :
    • 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33)
    Amino Acids and Derivatives Branched-chain amino acids Branched-Chain Amino Acid Biosynthesis  3-isopropylmalate dehydratase large subunit (EC 4.2.1.33)
    and 1 more
    Amino Acids and Derivatives Branched-chain amino acids Leucine Biosynthesis  3-isopropylmalate dehydratase large subunit (EC 4.2.1.33)
  • PFAM:
    no clan defined Aconitase; Aconitase family (aconitate hydratase) (PF00330; HMM-score: 491.4)
    and 1 more
    DUF521; Protein of unknown function (DUF521) (PF04412; HMM-score: 13.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: [4Fe-4S] cluster
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001455
    • TAT(Tat/SPI): 0.000137
    • LIPO(Sec/SPII): 0.000258
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MGQTLFDKVWNRHVLYGKLGEPQLLYIDLHLIHEVTSPQAFEGLRLQNRKLRRPDLTFATLDHNVPTIDIFNIKDEIANKQITTLQKNAIDFGVHIFDMGSDEQGIVHMVGPETGLTQPGKTIVCGDSHTATHGAFGAIAFGIGTSEVEHVFATQTLWQTKPKNLKIDINGTLPTGVYAKDIILHLIKTYGVDFGTGYALEFTGETIKNLSMDGRMTICNMAIEGGAKYGIIQPDDITFEYVKGRPFADNFAKSVDKWRELYSDDDAIFDRVIELDVSTLEPQVTWGTNPEMGVNFSEPFPEINDINDQRAYDYMGLEPGQKAEDIDLGYVFLGSCTNARLSDLIEASHIVKGNKVHPNITAIVVPGSRTVKKEAEKLGLDTIFKNAGFEWREPGCSMCLGMNPDQVPEGVHCASTSNRNFEGRQGKGARTHLVSPAMAAAAAIHGKFVDVRKVVV

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY* (repression) regulon
    CodY*(TF)important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)
  2. ↑ Charlotte D Majerczyk, Paul M Dunman, Thanh T Luong, Chia Y Lee, Marat R Sadykov, Greg A Somerville, Kip Bodi, Abraham L Sonenshein
    Direct targets of CodY in Staphylococcus aureus.
    J Bacteriol: 2010, 192(11);2861-77
    [PubMed:20363936] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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