PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS12325 [old locus tag: SACOL2347 ]
• pan locus tag^?: SAUPAN005852000
• symbol: SACOL_RS12325
• pan gene symbol^?: emrB
• synonym:
• product: MFS transporter

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS12325 [old locus tag: SACOL2347 ]
• symbol: SACOL_RS12325
• product: MFS transporter
• replicon: chromosome
• strand: -
• coordinates: 2406186..2408117
• length: 1932
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (2406186..2408117) NCBI
• BioCyc: SACOL_RS12325 BioCyc
• MicrobesOnline: see SACOL2347

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  1501
  1561
  1621
  1681
  1741
  1801
  1861
  1921
  
  ATGACTACGACCTTCATTATTAGCTACATTATTTTAGCGCTCATTATTGTTGGGGTTATC
  AATTTATTTTTAATAAGATCAAGAAAAAAAGGCAAACGCCAACAAAAGGAACAACAATTT
  ACGACACGTCAATCAAATCAATCTAAATTTAAAGCTAGTGATTTAGACAAAACAACTGAT
  CAATCGACACAACGTATGACGCATGAAGAGTTGCGTGTTGACAATCAAGATGATCATAGC
  CAAGTTAGTCTAAATGGTTACACAAAGGGGTCTGAGAAAGATCAAGAAGCATTCACTAAT
  AATAAGGATGAGGAAGCAGTTGCTGCTAAAAATCCTGAATCAGAAGAATATAAAGTGAAT
  GAAAAAATAAAAAAAGAACATAAAAACTTTATTTTTGGTGAAGGTGTTTCACGCGGTAAA
  ATATTAGCGGCATTATTATTCGGTATGTTTATTGCGATTTTAAACCAAACATTATTAAAT
  GTTGCTTTACCAAAAATTAATACGGAATTTAATATTTCTGCGTCAACAGGGCAATGGTTG
  ATGACGGGATTCATGTTAGTAAACGGTATTTTAATACCAATTACGGCGTATCTATTTAAT
  AAATATTCATATCGAAAATTATTTTTAGTTGCGTTAGTACTCTTTACAATTGGTTCATTA
  ATCTGTGCGATTTCAATGAATTTCCCAATCATGATGGTTGGACGTGTATTACAAGCCATT
  GGTGCAGGTGTACTAATGCCATTAGGTTCAATTGTTATTATTACAATTTATCCACCTGAA
  AAACGTGGTGCTGCCATGGGTACAATGGGTATCGCAATGATTTTAGCACCTGCAATTGGT
  CCAACATTATCAGGTTATATTGTACAAAATTACCATTGGAATGTGATGTTTTACGGTATG
  TTCATTATCGGGATTATCGCTATTTTAATTGGTTTTGTATGGTTTAAGTTATATCAATAT
  ACAACAAACCCTAAAGCTGATATCCCAGGTATCATTTTTAGTACGATTGGTTTTGGTGCT
  TTGTTATATGGTTTCTCAGAAGCTGGCAACAAAGGTTGGGGTTCAGTAGAGATAGAAACA
  ATGTTTGCGATTGGTATTATCTTTATTATTCTATTCGTTATTAGAGAATTAAGAATGAAA
  TCACCAATGTTGAATTTAGAAGTATTGAAATTCCCAACATTTACATTAACAACAATTATT
  AATATGGTTGTAATGTTAAGTTTATATGGTGGTATGATTTTATTACCGATTTATTTACAA
  AATTTACGCGGATTCTCAGCATTAGATTCCGGATTGTTATTATTACCTGGTTCTCTAATT
  ATGGGTCTACTAGGACCATTTGCAGGTAAATTGTTAGATACTATTGGTTTAAAACCATTA
  GCTATCTTTGGTATTGCTGTAATGACTTATGCAACATGGGAATTAACTAAATTAAATATG
  GATACACCATATATGACAATCATGGGTATCTATGTACTTCGTTCATTTGGTATGGCATTT
  ATAATGATGCCAATGGTAACTGCAGCTATTAATGCGTTACCGGGACGACTTGCCTCTCAT
  GGTAATGCTTTCTTAAATACGATGCGTCAATTAGCAGGCTCTATAGGTACAGCAATCTTA
  GTTACTGTAATGACAACACAAACTACACAACACTTATCAGCTTTTGGGGAAGAGTTAGAT
  AAAACGAATCCTGTTGTACAAGATCATATGCGTGAATTAGCATCACAATATGGCGGACAA
  GAAGGCGCAATGAAAGTGTTACTACAATTTGTAAATAAACTAGCAACGGTTGAAGGTATT
  AATGATGCATTTATAGTTGCAACGATATTTAGCATCATCGCCTTAATTTTATGTTTATTT
  TTACAAAGTAATAAAAAAGCAAAAGCTACAGCTCAAAAGTTAGATGCAGATAATAGTATC
  AATCATGAATAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1440
  1500
  1560
  1620
  1680
  1740
  1800
  1860
  1920
  1932
  

⊟Protein

⊟General

• locus tag: SACOL_RS12325 [old locus tag: SACOL2347 ]
• symbol: SACOL_RS12325
• description: MFS transporter
• length: 643
• theoretical pI: 9.56274
• theoretical MW: 70977.5
• GRAVY: 0.398134

⊟Function

• TIGRFAM:
  Cellular processes Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 443.1)
  Metabolism Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 443.1)
  and 11 more
  multidrug resistance protein (TIGR00880; HMM-score: 76.8)
  drug resistance transporter, Bcr/CflA subfamily (TIGR00710; HMM-score: 67.8)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-galactonate transporter (TIGR00893; HMM-score: 64.7)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids phosphoglycerate transporter family protein (TIGR00881; HMM-score: 63.8)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, aromatic acid:H+ symporter (AAHS) family (TIGR00895; HMM-score: 48.1)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, sialate:H+ symporter (SHS) family (TIGR00891; HMM-score: 25.6)
  oxalate/formate antiporter (TIGR04259; HMM-score: 22.7)
  Metabolism Transport and binding proteins Cations and iron carrying compounds cation transporter family protein (TIGR00860; HMM-score: 13.9)
  Cellular processes Cellular processes Adaptations to atypical conditions phage shock protein B (TIGR02976; HMM-score: 10)
  synaptic vesicle protein SV2 (TIGR01299; HMM-score: 8.3)
  sodium-independent organic anion transporter (TIGR00805; HMM-score: 7.2)
• TheSEED: see SACOL2347
• PFAM:
  MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 192.3)
  and 11 more
  Sugar_tr; Sugar (and other) transporter (PF00083; HMM-score: 35.5)
  no clan defined DUF4834; Domain of unknown function (DUF4834) (PF16118; HMM-score: 16.6)
  Neur_chan_memb; Neurotransmitter-gated ion-channel transmembrane region (PF02932; HMM-score: 14.6)
  EphA2_TM; Ephrin type-A receptor 2 transmembrane domain (PF14575; HMM-score: 14.1)
  ATP_synthase (CL0255) YMF19; Plant ATP synthase F0 (PF02326; HMM-score: 13.4)
  no clan defined ASFV_J13L; African swine fever virus J13L protein (PF05568; HMM-score: 12.3)
  DUF2561; Protein of unknown function (DUF2561) (PF10812; HMM-score: 12.2)
  Shisa; Wnt and FGF inhibitory regulator (PF13908; HMM-score: 10.9)
  DUF1180; Protein of unknown function (DUF1180) (PF06679; HMM-score: 10.7)
  DUF1510; Protein of unknown function (DUF1510) (PF07423; HMM-score: 9)
  MFS (CL0015) OATP; Organic Anion Transporter Polypeptide (OATP) family (PF03137; HMM-score: 5.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 15
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.083803
  • TAT(Tat/SPI): 0.001799
  • LIPO(Sec/SPII): 0.024049
• predicted transmembrane helices (TMHMM): 15

⊟Accession numbers

• GI: 446136019 NCBI
• RefSeq: WP_000213874 NCBI
• UniProt: see SACOL2347

⊟Protein sequence

• MTTTFIISYIILALIIVGVINLFLIRSRKKGKRQQKEQQFTTRQSNQSKFKASDLDKTTDQSTQRMTHEELRVDNQDDHSQVSLNGYTKGSEKDQEAFTNNKDEEAVAAKNPESEEYKVNEKIKKEHKNFIFGEGVSRGKILAALLFGMFIAILNQTLLNVALPKINTEFNISASTGQWLMTGFMLVNGILIPITAYLFNKYSYRKLFLVALVLFTIGSLICAISMNFPIMMVGRVLQAIGAGVLMPLGSIVIITIYPPEKRGAAMGTMGIAMILAPAIGPTLSGYIVQNYHWNVMFYGMFIIGIIAILIGFVWFKLYQYTTNPKADIPGIIFSTIGFGALLYGFSEAGNKGWGSVEIETMFAIGIIFIILFVIRELRMKSPMLNLEVLKFPTFTLTTIINMVVMLSLYGGMILLPIYLQNLRGFSALDSGLLLLPGSLIMGLLGPFAGKLLDTIGLKPLAIFGIAVMTYATWELTKLNMDTPYMTIMGIYVLRSFGMAFIMMPMVTAAINALPGRLASHGNAFLNTMRQLAGSIGTAILVTVMTTQTTQHLSAFGEELDKTNPVVQDHMRELASQYGGQEGAMKVLLQFVNKLATVEGINDAFIVATIFSIIALILCLFLQSNKKAKATAQKLDADNSINHE

⊟Experimental data

• experimentally validated: see SACOL2347
• protein localization: see SACOL2347
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications