From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS13340 [old locus tag: SACOL2548 ]
  • pan locus tag?: SAUPAN006188000
  • symbol: SACOL_RS13340
  • pan gene symbol?: —
  • synonym:
  • product: EamA family transporter

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS13340 [old locus tag: SACOL2548 ]
  • symbol: SACOL_RS13340
  • product: EamA family transporter
  • replicon: chromosome
  • strand: +
  • coordinates: 2605677..2606612
  • length: 936
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (2605677..2606612) NCBI
  • BioCyc: SACOL_RS13340 BioCyc
  • MicrobesOnline: see SACOL2548

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    GTGAGATACTTGGCGAATAATCATTCAGCTTTGCGTTTAAAAGGAATTATACTTGCCATT
    GTCGGTGCTTGTTTATGGGGATTAGGTGGTACTGTTTCTGATTTCTTGTTCAAATATAAG
    AATATTAATGTCGATTGGTACGTCACTGCTCGACTTGTAGTCAGTGGTGTTTTCTTACTT
    ATTATGTACAAAATGATGCAACCCAAACGTTCAATATTTAGCGTATTCCAAGATCGACGT
    ATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTAT
    ATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCA
    GTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTG
    CTTGCTATTATCATGACACTATTAGGAACATTTTTATTATTAACAAATGGTTCATTTTCT
    AATTTAGTCGTCAATCCTGCAAGTTTATTCTGGGGTATTTTAGCTGGTGTAGCACTCGCT
    TTTTACACAATTTATCCTTCAGACCTACTTAACCGCTTCGGTTCGATTCTAATTGTCGGG
    TGGGCAATGCTTATTTCTGGTGTTGCGATGAATTTACGCCATCCAATTTGGCACATTGAT
    ATCACTAAATGGGACATATCAATTATATTATTTTTAATCTTTGGTATTATCGGTGGTACC
    GCACTCGCATTTTATTTCTTTATCGACAGTTTACAATACATATCAGCGAAAGAAACAACA
    TTATTCGGAACTGTTGAACCTGTCGTAGCCGTTATCGCAAGCAGTCTATGGTTACATGTG
    GCATTCAAACCATTTCAAATCGTAGGCATCATTCTTATTATGATTTTAATTTTATTACTA
    TCACTTAAAAGACAACCTGAAACATTAGATGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    936

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL_RS13340 [old locus tag: SACOL2548 ]
  • symbol: SACOL_RS13340
  • description: EamA family transporter
  • length: 311
  • theoretical pI: 9.92024
  • theoretical MW: 34743.7
  • GRAVY: 1.01383

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Amino acids, peptides and amines carboxylate/amino acid/amine transporter (TIGR00950; HMM-score: 108.2)
  • TheSEED: see SACOL2548
  • PFAM:
    DMT (CL0184) EamA; EamA-like transporter family (PF00892; HMM-score: 129.1)
    and 1 more
    no clan defined COX14; Cytochrome oxidase c assembly (PF14880; HMM-score: 10.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 10
  • LocateP:
  • SignalP: Signal peptide LIPO(Sec/SPII) length 23 aa
    • SP(Sec/SPI): 0.025227
    • TAT(Tat/SPI): 0.000375
    • LIPO(Sec/SPII): 0.77052
    • Cleavage Site: CS pos: 23-24. VGA-CL. Pr: 0.7512
  • predicted transmembrane helices (TMHMM): 10

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MRYLANNHSALRLKGIILAIVGACLWGLGGTVSDFLFKYKNINVDWYVTARLVVSGVFLLIMYKMMQPKRSIFSVFQDRRMLGKLLIFSILGMLVVQYAYMASINTGNAAIATLLQYIAPVYIIIWFVIRGVAKLTLFDVLAIIMTLLGTFLLLTNGSFSNLVVNPASLFWGILAGVALAFYTIYPSDLLNRFGSILIVGWAMLISGVAMNLRHPIWHIDITKWDISIILFLIFGIIGGTALAFYFFIDSLQYISAKETTLFGTVEPVVAVIASSLWLHVAFKPFQIVGIILIMILILLLSLKRQPETLDE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: see SACOL2548
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SACOL_RS13340&oldid=95048"