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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL_RS13685 [old locus tag: SACOL2616 ]
- pan locus tag?: SAUPAN006294000
- symbol: SACOL_RS13685
- pan gene symbol?: panE
- synonym:
- product: 2-dehydropantoate 2-reductase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL_RS13685 [old locus tag: SACOL2616 ]
- symbol: SACOL_RS13685
- product: 2-dehydropantoate 2-reductase
- replicon: chromosome
- strand: +
- coordinates: 2672486..2673346
- length: 861
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
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841ATGTTATCAGTTGCGATTATCGGCCCAGGCGCTGTCGGTACAACTATTGCCTATGAATTA
CAACAATCATTGCCTCATACAACGCTTATCGGGAGACACGCCAAAACAATAACATATTAT
ACTGTACCACATGCACCTGCACAAGATATTGTTGTGAAAGGTTATGAAGATGTCACAAAC
ACATTTGATGTTATTATTATTGCAGTCAAAACACATCAACTTGATGCAGTTATTCCTCAT
TTAACTCATCTTGCACATGAGGACACGCTCATCATTTTAGCCCAAAATGGTTATGGTCAA
CTCGAACATATTTCATTTAAAAACGTATGCCAAGCAGTTGTCTATATAAGTGGTCAAAAG
AAAGGCGATGTTGTTACGCACTTCAGAGATTATCAATTACGTATACAAGATAATGCATTA
ACTCGACAATTTAGAGATTTGGTCCAAGACAGTCAAATCGACATTGTATTAGAAGCAAAT
ATTCAACAAGCTATTTGGTATAAATTGTTAGTTAACTTGGGCATTAATTCTATCACAGCA
CTCGGTAGACAGACCGTTGCAATCATGCATAATCCTGAAATACGTACACTGTGTAGACAG
CTATTACTAGATGGTTGTCGTGTTGCACAGGCAGAGGGGTTAAACTTTTCAGAACAAACT
GTCGATACTATTATGACTATATATCAAGGTTATCCCGACGAAATGGGAACGAGCATGTAT
TACGACATTGCGCATCAGCAACCTCTTGAAGTCGAAGCGATTCAAGGCTTTATTTATCGA
CGTGCACGTGAACACAATCTTGACACCCCATATCTTGATACTATTTATAGCTTTTTACGC
GCGTATCAACAAAATATGTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL_RS13685 [old locus tag: SACOL2616 ]
- symbol: SACOL_RS13685
- description: 2-dehydropantoate 2-reductase
- length: 286
- theoretical pI: 6.06642
- theoretical MW: 32357.7
- GRAVY: -0.0632867
⊟Function[edit | edit source]
- reaction: EC 1.1.1.169? ExPASy2-dehydropantoate 2-reductase (R)-pantoate + NADP+ = 2-dehydropantoate + NADPH
- TIGRFAM: Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A 2-dehydropantoate 2-reductase (TIGR00745; EC 1.1.1.-; HMM-score: 176)and 1 moreAmino acid biosynthesis Glutamate family pyrroline-5-carboxylate reductase (TIGR00112; EC 1.5.1.2; HMM-score: 16.2)
- TheSEED: see SACOL2616
- PFAM: 6PGD_C (CL0106) ApbA_C; Ketopantoate reductase PanE/ApbA C terminal (PF08546; HMM-score: 104.4)and 9 moreNADP_Rossmann (CL0063) ApbA; Ketopantoate reductase PanE/ApbA (PF02558; HMM-score: 63.8)F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 29.5)NAD_Gly3P_dh_N; NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus (PF01210; HMM-score: 25.4)DAO; FAD dependent oxidoreductase (PF01266; HMM-score: 16.7)NAD_binding_2; NAD binding domain of 6-phosphogluconate dehydrogenase (PF03446; HMM-score: 16)Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 15.2)Rossmann-like; Rossmann-like domain (PF10727; HMM-score: 14.4)IlvN; Acetohydroxy acid isomeroreductase, NADPH-binding domain (PF07991; HMM-score: 14.3)Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 11.8)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 1.78
- Cytoplasmic Membrane Score: 8.16
- Cellwall Score: 0.06
- Extracellular Score: 0.01
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.090277
- TAT(Tat/SPI): 0.003487
- LIPO(Sec/SPII): 0.016301
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MLSVAIIGPGAVGTTIAYELQQSLPHTTLIGRHAKTITYYTVPHAPAQDIVVKGYEDVTNTFDVIIIAVKTHQLDAVIPHLTHLAHEDTLIILAQNGYGQLEHISFKNVCQAVVYISGQKKGDVVTHFRDYQLRIQDNALTRQFRDLVQDSQIDIVLEANIQQAIWYKLLVNLGINSITALGRQTVAIMHNPEIRTLCRQLLLDGCRVAQAEGLNFSEQTVDTIMTIYQGYPDEMGTSMYYDIAHQQPLEVEAIQGFIYRRAREHNLDTPYLDTIYSFLRAYQQNM
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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