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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00062
  • pan locus tag?: SAUPAN000903000
  • symbol: SAOUHSC_00062
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00062
  • symbol: SAOUHSC_00062
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 68201..69073
  • length: 873
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGAATAAACTTCGAGACACTACTTTTCTATCATATTTATTTACTATCATATTGTGGGGA
    TCTGCATTTCCAATGATAAAGATTGCGTTAAATGATTTTAGTGCAGAATCATTGTCGGCA
    TTTCGTTTAATTTTAGCAACAATAATTCTCTTGCCGTTCGTAATTATAAAGAAATTGCCT
    ACCCCTGAACTAAGAGATATCCCTGTTATTTTTATTTTAGGATTTTGTGGATTTGTGATA
    TATCACACAGCTTTAAATTTTGGTGAAACTTTGATTAGTGCAGGTATATCTGGAATTCTA
    GTCTCTACAACGCCTATTTTTTCTAGTGCTTTAGCTTATATATTTTTAAAAGAGCATTTT
    TCAAAATGGAATTGGTTAAGTTCACTCGTCGCATTTATAGGAATATCCATTATTTCAATA
    AGTAAAGATGATTACACAACTATTAATGTATTAGGTGTTTTTATTATTTTACTTGCATCT
    TTTAGTGAAAGTTTGTATTTTACTTTCCAGAAAAAATACATAGAAAAATACGGCTTCATC
    GCTTTCACACTATATACAATAATGGCAAGCTCACCATTTATGCTTATTTTTATTCCTGAA
    ATCATCAACGATATACACGGCGCCACTTTTACATCAATAGTATCGGTACTTTATTTAGCT
    ATATTCCCTACTATAATTCCATACGTTTTGCTTGCTTATATTGTGAAGTCAGTTGGTGTC
    TCTGATGCAACAATGTCTCTTTATTTAACACCTATCGTTTCTTTATTATTATCTTATCTG
    TTATTAGACGAGCTACCAACAACCCTTGCTATTATAGGCGGAATTATCACTCTACTAGGC
    GTTAGTTTAAGTAACTTCTTTCAAAATACATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    873

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_00062
  • symbol: SAOUHSC_00062
  • description: hypothetical protein
  • length: 290
  • theoretical pI: 6.2511
  • theoretical MW: 32209.2
  • GRAVY: 1.03483

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Amino acids, peptides and amines carboxylate/amino acid/amine transporter (TIGR00950; HMM-score: 110.3)
    and 2 more
    Hypothetical proteins Conserved Tpt phosphate/phosphoenolpyruvate translocator (TIGR00817; HMM-score: 17.9)
    Metabolism Transport and binding proteins Unknown substrate protein RarD (TIGR00688; HMM-score: 15.8)
  • TheSEED  :
    • Permease of the drug/metabolite transporter (DMT) superfamily
    RNA Metabolism RNA processing and modification Queuosine-Archaeosine Biosynthesis  Permease of the drug/metabolite transporter (DMT) superfamily
    and 1 more
    Virulence, Disease and Defense Resistance to antibiotics and toxic compounds Cobalt-zinc-cadmium resistance  Permease of the drug/metabolite transporter (DMT) superfamily
  • PFAM:
    DMT (CL0184) EamA; EamA-like transporter family (PF00892; HMM-score: 154.7)
    and 2 more
    TPT; Triose-phosphate Transporter family (PF03151; HMM-score: 24.4)
    EmrE; Putative multidrug resistance efflux transporter (PF13536; HMM-score: 20.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 10
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.057613
    • TAT(Tat/SPI): 0.009079
    • LIPO(Sec/SPII): 0.018061
  • predicted transmembrane helices (TMHMM): 10

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNKLRDTTFLSYLFTIILWGSAFPMIKIALNDFSAESLSAFRLILATIILLPFVIIKKLPTPELRDIPVIFILGFCGFVIYHTALNFGETLISAGISGILVSTTPIFSSALAYIFLKEHFSKWNWLSSLVAFIGISIISISKDDYTTINVLGVFIILLASFSESLYFTFQKKYIEKYGFIAFTLYTIMASSPFMLIFIPEIINDIHGATFTSIVSVLYLAIFPTIIPYVLLAYIVKSVGVSDATMSLYLTPIVSLLLSYLLLDELPTTLAIIGGIITLLGVSLSNFFQNT

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • data available for N315

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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