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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00109
  • pan locus tag?: SAUPAN000965000
  • symbol: SAOUHSC_00109
  • pan gene symbol?: —
  • synonym:
  • product: replication initiation protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00109
  • symbol: SAOUHSC_00109
  • product: replication initiation protein
  • replicon: chromosome
  • strand: +
  • coordinates: 114083..114568
  • length: 486
  • essential: no DEG

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGTCTAACGCAGAGAGACTTTCACATTTTATGAGTACTAATCCTGAAATTCGGCTTTGG
    GATATTTTACAAACAAACTTTAAAGCTAAAGCTCTTAAAGAAAAAGTTTATATTGAATAT
    GACAAAATAAAAGCAACTCTTTGGAATAGACGTAGTATGCGCGTTGAATTTAATCCTAAT
    AAGCTTTCGCATGATGAAGTGCTTTGGTTAAAACAAAATATCATCAGTTATTTGGACGAT
    GTTAGTTTTACGAGATTAGATTTGGCTTTTGATTTTGAATTTGATTTAAATGACTATTAT
    GCATTGTCAGATAAGTCGGTAAAGAAAACTATATTTTATGGACGTAATGTAAAACCAGAA
    ACAAAATATTTTGGTGTGCGTAATAGTGATAGGTTTATTCGGATTTATAATAAAAACAAG
    AACGTAAAGATAATGCAGATGTTGAAATTGATTCAACATTTCTATGGCGTGTGGAAATTG
    AATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    486

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_00109
  • symbol: SAOUHSC_00109
  • description: replication initiation protein
  • length: 161
  • theoretical pI: 10.1441
  • theoretical MW: 19509.4
  • GRAVY: -0.550311

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • Replication initiation protein, topisomerase
    Phages, Prophages, Transposable elements, Plasmids Plasmid related functions Rolling-circle replication  Replication initiation protein, topisomerase
  • PFAM:
    TBP-like (CL0407) Rep_trans; Replication initiation factor (PF02486; HMM-score: 22.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003086
    • TAT(Tat/SPI): 0.000328
    • LIPO(Sec/SPII): 0.000314
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSNAERLSHFMSTNPEIRLWDILQTNFKAKALKEKVYIEYDKIKATLWNRRSMRVEFNPNKLSHDEVLWLKQNIISYLDDVSFTRLDLAFDFEFDLNDYYALSDKSVKKTIFYGRNVKPETKYFGVRNSDRFIRIYNKNKNVKIMQMLKLIQHFYGVWKLN

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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