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NCBI: 03-AUG-2016
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus NCTC8325
- locus tag: SAOUHSC_00246
- pan locus tag?: SAUPAN001161000
- symbol: SAOUHSC_00246
- pan gene symbol?: —
- synonym:
- product: drug transporter
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAOUHSC_00246
- symbol: SAOUHSC_00246
- product: drug transporter
- replicon: chromosome
- strand: -
- coordinates: 264825..266201
- length: 1377
- essential: no DEG other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3919266 NCBI
- RefSeq: YP_498841 NCBI
- BioCyc: G1I0R-229 BioCyc
- MicrobesOnline: 1288735 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1321GTGAATAACACACAATCTTCACCACGCAGTAATATTATTATTGCGATTATGTTGTCTGCA
TTAACATATTGGTTGTTTGCACAATCATTTATTAATATAGGACCTCTCGTTGGTCAAACA
TATCAAACCTCTCCTGCCGTGTTAAATTTATCTATTAGTTTAACTTCCTTCGCCACAGGT
ATCTTCATGGTGGCTGCAGGTGATATTGCTGATAAAATAGGACAACTGAGAATGACATAC
ATGGGTCTCATAATCAGTATGTTTGCATCTCTTCTATTAATTATATCGGACATCACTGCA
CTGCTCATCATCGGTAGAATTTTACAAGGTCTATCAGCAGCTATCTTGTTACCTTCAACA
GTTGGCGTGTTAAATAATCAATTTAAAGGAGAACATTTAAGACGAGCGATTAGTTATCTA
ATGATTAGTACTGTTGGTGGCATCGGCCTAGCTGGTGTTATCGGCGGTTTAATTGCCTCA
AATTTCGGATGGCAAACGAATTTCATCATTAGTATAGTCATTGCTTTCATTGCCATATTG
CTTCTAAAAGGCACACCTGAAAAAGTAAGTCAACATAGCCACCGTCATCCATTCGATTAC
AAAGGTATGTCGATTTTCGCTGTTATGATTGGTAGCTTTACATTATTGTTAACACAAGGA
TTCGAACAAGGTTGGTTTAGTACATTTTCAATCATTTGTCTGAGCATTTTTATCATCACT
ACGTTGATATTCATCATCATCGAACGTCGACATGAAGTACCTTTTATTGATTTCTCAGTA
TTACGCAACCGTCCGTTCATTGGTGCATTTTTAAATAACTTTGTTTTAAATAGCGGTCTA
GGCGTAACAGTGGTCTTTTTCATATATGCTCAAACACACCTTGGTTTATCAGCTGCGCAA
TCTGGACTTGTTACATTGCCATATGCCATTGTGGCAGTTGCGATGATTCGTTTAGGTGAA
AAAGCAACATTACGTTTCGGTGGCAAATTGATGCTCATCATTGGTCCGTTGTTCCCTGTC
ATCGGCATCACTATTATTAGCATGACTCAATTATTGGCATCACAATATGTCATTGCAGTT
ATCATTGGTTTCGTCATATGTGCGATAGGTAATGGTTTAGTCGCAACACCTGGACTTACG
ATTGCAATTTTCAGTATGCCTAATGAAAAAGTTGGTTTAGCTACAGGATTATATAAAATG
AGTGGTACATTAGGTGGCTCCTTTGGTATAGCACTAAGTACTACAGTTTTCAGTATGTTA
CAACTAAACTATGCACCAAGTGTAGCTGCAACCGTAACATTTATAGTCAGCATTGTATTG
ATGATCCTTGGCTCATTGTCTGCATACATGATCATTCCAAAAACAGTTAAATCTTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAOUHSC_00246
- symbol: SAOUHSC_00246
- description: drug transporter
- length: 458
- theoretical pI: 10.1683
- theoretical MW: 49056.3
- GRAVY: 0.974891
⊟Function[edit | edit source]
- TIGRFAM: Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 133.3)Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 133.3)and 6 moreTransport and binding proteins Carbohydrates, organic alcohols, and acids D-galactonate transporter (TIGR00893; HMM-score: 55.4)multidrug resistance protein (TIGR00880; HMM-score: 45.4)drug resistance transporter, Bcr/CflA subfamily (TIGR00710; HMM-score: 40.9)Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, aromatic acid:H+ symporter (AAHS) family (TIGR00895; HMM-score: 31.5)Transport and binding proteins Carbohydrates, organic alcohols, and acids oxalate/formate antiporter family transporter (TIGR00890; HMM-score: 21)Transport and binding proteins Carbohydrates, organic alcohols, and acids sugar efflux transporter (TIGR00899; HMM-score: 18.1)
- TheSEED :
- FIG01108109: hypothetical protein
- PFAM: MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 137.1)and 1 moreno clan defined UL42; HCMV UL42 (PF17638; HMM-score: 13.8)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 14
- LocateP: Multi-transmembrane
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0.17
- Signal peptide possibility: 0.5
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.123294
- TAT(Tat/SPI): 0.003305
- LIPO(Sec/SPII): 0.01869
- predicted transmembrane helices (TMHMM): 14
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MNNTQSSPRSNIIIAIMLSALTYWLFAQSFINIGPLVGQTYQTSPAVLNLSISLTSFATGIFMVAAGDIADKIGQLRMTYMGLIISMFASLLLIISDITALLIIGRILQGLSAAILLPSTVGVLNNQFKGEHLRRAISYLMISTVGGIGLAGVIGGLIASNFGWQTNFIISIVIAFIAILLLKGTPEKVSQHSHRHPFDYKGMSIFAVMIGSFTLLLTQGFEQGWFSTFSIICLSIFIITTLIFIIIERRHEVPFIDFSVLRNRPFIGAFLNNFVLNSGLGVTVVFFIYAQTHLGLSAAQSGLVTLPYAIVAVAMIRLGEKATLRFGGKLMLIIGPLFPVIGITIISMTQLLASQYVIAVIIGFVICAIGNGLVATPGLTIAIFSMPNEKVGLATGLYKMSGTLGGSFGIALSTTVFSMLQLNYAPSVAATVTFIVSIVLMILGSLSAYMIIPKTVKS
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas [1] [2]
- protein localization:
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- predicted SigA promoter [3] : S80 < SAOUHSC_00246 < S81
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: [3] Multi-gene expression profiles
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
Proteomics: 2015, 15(21);3648-61
[PubMed:26224020] [WorldCat.org] [DOI] (I p) - ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
Sci Rep: 2017, 7(1);9718
[PubMed:28887440] [WorldCat.org] [DOI] (I e) - ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
PLoS Genet: 2016, 12(4);e1005962
[PubMed:27035918] [WorldCat.org] [DOI] (I e)