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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00294
  • pan locus tag?: SAUPAN001238000
  • symbol: SAOUHSC_00294
  • pan gene symbol?: nanT
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00294
  • symbol: SAOUHSC_00294
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 307111..308643
  • length: 1533
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGAAAGAAGTTGGATTTGGCACACTAAACTGGGTTGCCGTTATCATTTATCTACTAGCT
    ATGTTGTTCATTGGCGTTTATTTTACCAAGCGCGCGAGCCAAAGTACCAATAGTTTCTTT
    ACCGCAAGTGGTCGCTTGCCATCTTGGGTAGTTGGCTTTTCAATTTATGCTACTACGTTA
    AGTGCGATTACATTTATGTCGACACCAGAGAAAGCATTTTTAACAGATTGGTCATATATC
    GCTGGTAACATTGCTATCGTCGCAATTATTCCATTACTTATTTATTTCTATGTCCCTTTC
    TTTAAAAAGTTAAAGGTAACATCTGCATATGAATATTTAGAAGCTAGATTTGGCCCTAGC
    ATACGTGTCATTGGCTCATTATTATTTGTCGTTTACCATTTAGGGCGTGTTGCAATTGTT
    ATCTACTTACCAACATTAGCAATCACATCTGTATCAGACATGAACCCTTATATCGTTGCA
    TCACTCGTTGGTTTACTATGTATTTTATATACATTTTTAGGTGGTTTCGAAGGTGTGGTT
    TGGAGTGATTTCATTCAAGGCGTCATTTTATTAGGCGGCGCTTTAGTTATTATTATTCTA
    GGTGTTGTGAACATTAAAGGCGGTTTCGGCACTGTCTTTGCAGATGCGATTGAGCACAAA
    AAATTAATTAGTGCAGACAATTGGAAACTAAATACTGCGGCAGCTGCCATTCCAATTATT
    TTCCTAGGAAATATTTTCAACAACTTGTATCAATACACAGCGAGTCAAGACGTCGTGCAG
    CGTTATCAAGCTTCTGATAGTTTAAAAGAAACAAATAAATCGTTATGGACAAATGGTATC
    CTAGCTTTAATTTCAGCACCCTTATTTTATGGTATGGGTACAATGCTGTATTCATTTTAT
    ACACATGAAGCTGTTTTACCAAAAGGCTTCAATACATCATCTGTAGTGCCATATTTCATT
    TTGACTGAGATGCCACCATTTGTAGCAGGATTACTTATTGCAGCCATTTTCGCCGCTGCA
    CAGTCTACCATTTCATCTAGTTTAAATTCTATATCTGCTTGTATTTCAATCGACATTAAG
    CAACGCTTCTTCGGAAAAGGTAGCGAGCGACACGAAGTTAACTTTGCACGTTTCATTATT
    ATCATTGCAGGTATTTTCGGTTTTGGAATGTCACTATACTTAATTGCTTCTAATTCAAAT
    GACTTATGGGATTTATTCTTGTTTGTGACTGGATTATTCGGCGTTCCATTGGCTGGTGTA
    TTTGCAGTTGGTATTTTCACTAAACGTACGAATACATTCGGTGTTATTTGTGGATTAATA
    TTGGGTATCATCTTTGCTTATGTCTATAATGGTGTTGGCAAAGGTAACTCACCTTTCTAT
    GTATCTACCATTTCATTTACAGTTGCTTTTGTCTTTGCTTATATACTTAGCTTCATTGTC
    CCTTCAAAACATAAAAAAGATATAACGGGATTAACAATTTTTGAAAAAGATAAACCATCA
    ACATACATTTCAAAAACGGCTACGAAAAAGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1533

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_00294
  • symbol: SAOUHSC_00294
  • description: hypothetical protein
  • length: 510
  • theoretical pI: 9.58689
  • theoretical MW: 55813.2
  • GRAVY: 0.731569

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds transporter, solute:sodium symporter (SSS) family (TIGR00813; HMM-score: 220.4)
    and 6 more
    Metabolism Transport and binding proteins Amino acids, peptides and amines sodium/proline symporter (TIGR02121; HMM-score: 97)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A sodium/pantothenate symporter (TIGR02119; HMM-score: 71.5)
    Metabolism Transport and binding proteins Other sodium/pantothenate symporter (TIGR02119; HMM-score: 71.5)
    probable sodium:solute symporter, VC_2705 subfamily (TIGR03648; HMM-score: 57.4)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids cation/acetate symporter ActP (TIGR02711; HMM-score: 31.9)
    Metabolism Transport and binding proteins Cations and iron carrying compounds multicomponent Na+:H+ antiporter (TIGR00942; HMM-score: 14.1)
  • TheSEED  :
    • Predicted sialic acid transporter
    Cell Wall and Capsule Capsular and extracellular polysacchrides Sialic Acid Metabolism  Predicted sialic acid transporter
  • PFAM:
    APC (CL0062) SSF; Sodium:solute symporter family (PF00474; HMM-score: 140.4)
    and 2 more
    CopD_like (CL0430) UPF0093; Uncharacterised protein family (UPF0093) (PF03653; HMM-score: 12.5)
    no clan defined LRR19-TM; Leucine-rich repeat family 19 TM domain (PF15176; HMM-score: 8.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.043374
    • TAT(Tat/SPI): 0.001183
    • LIPO(Sec/SPII): 0.007584
  • predicted transmembrane helices (TMHMM): 13

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKEVGFGTLNWVAVIIYLLAMLFIGVYFTKRASQSTNSFFTASGRLPSWVVGFSIYATTLSAITFMSTPEKAFLTDWSYIAGNIAIVAIIPLLIYFYVPFFKKLKVTSAYEYLEARFGPSIRVIGSLLFVVYHLGRVAIVIYLPTLAITSVSDMNPYIVASLVGLLCILYTFLGGFEGVVWSDFIQGVILLGGALVIIILGVVNIKGGFGTVFADAIEHKKLISADNWKLNTAAAAIPIIFLGNIFNNLYQYTASQDVVQRYQASDSLKETNKSLWTNGILALISAPLFYGMGTMLYSFYTHEAVLPKGFNTSSVVPYFILTEMPPFVAGLLIAAIFAAAQSTISSSLNSISACISIDIKQRFFGKGSERHEVNFARFIIIIAGIFGFGMSLYLIASNSNDLWDLFLFVTGLFGVPLAGVFAVGIFTKRTNTFGVICGLILGIIFAYVYNGVGKGNSPFYVSTISFTVAFVFAYILSFIVPSKHKKDITGLTIFEKDKPSTYISKTATKK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: NanR* (repression) regulon, CcpA* regulon
    NanR*(TF)important in Sialic acid utilization, N-acetylglucosamine utilization; RegPrecise    transcription unit transferred from N315 data RegPrecise 
    CcpA*(TF)important in Carbon catabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Michael E Olson, Jessica M King, Timothy L Yahr, Alexander R Horswill
Sialic acid catabolism in Staphylococcus aureus.
J Bacteriol: 2013, 195(8);1779-88
[PubMed:23396916] [WorldCat.org] [DOI] (I p)

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