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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00327
  • pan locus tag?: SAUPAN001884000
  • symbol: SAOUHSC_00327
  • pan gene symbol?: fepC
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00327
  • symbol: SAOUHSC_00327
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 339971..341653
  • length: 1683
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGCTAATAACTGCTGCTATGGTGTGTAGCTTTGGGTTACTGAAAAGTCAGGCAGCAGAA
    CAACAAAGTATTAGTGATGTATATAGTGTGATAACGGATGCGAAATCTGCACTTTCTAAT
    AATTCGATATCGAATGACAATAAGCAGAAAGCAATTGAGCAAGTGGTAAGTGCAGTTAAG
    AAATTATCGCTTGAAGATAATAGTGAAAGTAATGCTGTCAAATCAGATGTGAGAAAGCTT
    GAAGATGCAAAAGCGAATGATAATCAAAAAGATACACTTTCGCAATTAACGAAGTCATTA
    ATTGCTTATGAAGAGAAATTGGCTAGTAAAGATGCGGGTTCTAAAATTAAACTATTGCAA
    CAGCAAGTCGATGCTAAAGATGCTGCGATGACAAAAGCGATTAAAGATAAAAATAAAGCG
    GAATTAGAATCTTTGAACAATAGTTTGAATCAGATTTGGACAAGTAATGAAACAGTGATT
    CGCAATTATGACGCAAATCAATATGGACAAATTGAAGTCGCATTATTACAACTTAGAATT
    GCAATTCATAAGTCACCATTAGATACGGCAAAAGTGTCACATGCTTGGACAACTTTTAAA
    TCAAATATTGATCATGTCGATAAAAAAAGTAATACGTCTGCAAATGATCAATACCATGTA
    TCACAATTAAATGATGCGTTAGAGAAGGCGATTAAAGCTATCGACGACAATCAATTGTCG
    GATGCTGATGCTGCGCTTACACATTTTATAGAAACTTGGCCGTATGTTGAAGGTCAAATT
    CAAACTAAAGACGGTGCTTTGTATACGAAAATTGAAGATAAAATACCATATTATCAAAGT
    GTATTAGACGAACATAATAAAGCACATGTGAAAGATGGTTTAGTAGATTTAAATAACCAA
    ATTAAAGAGGTTGTTGGCCATAGTTATAGCTTCGTCGATGTGATGATTATCTTTTTACGT
    GAAGGGCTAGAAGTGTTGTTAATTGTAATGACATTGACTACCATGACGCGTAATGTAAAA
    GATAAGAAAGGGACTGCAAGTGTGATTGGTGGTGCAATTGCCGGACTTGTACTGAGTATT
    ATCTTAGCAATTACGTTTGTAGAAACTTTAGGGAATAGTGGCATTCTTCGTGAAAGTATG
    GAAGCGGGATTAGGTATCGTTGCGGTCATATTAATGTTTATCGTTGGTGTTTGGATGCAC
    AAACGTTCAAATGCAAAACGTTGGAATGACATGATTAAAAATATGTATGCTAATGCGATT
    AGTAATGGTAATTTGGTATTGTTAGCGACGATTGGTTTAATATCTGTGTTGCGTGAAGGT
    GTCGAGGTTATCATTTTCTATATGGGGATGATAGGTGAGCTAGCGACCAAAGATTTTATT
    ATTGGTATTGCTTTAGCTATCGTTATTTTAATCATCTTTGCATTATTATTTAGATTTATA
    GTTAAATTAATACCTATTTTCTATATATTTAGAGTGTTGTCGATCTTTATTTTTATTATG
    GGATTCAAAATGCTTGGCGTAAGTATTCAAAAGTTACAATTATTAGGTGCGATGCCAAGA
    CATGTTATTGAAGGATTCCCAACGATTAACTGGTTGGGCTTTTATCCAAGTTATGAACCA
    TTGATAGCACAAGGTGCTTATATTATGGTAGTTGCTATCTTAATCTTTAAATTTAAAAAA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1683

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_00327
  • symbol: SAOUHSC_00327
  • description: hypothetical protein
  • length: 560
  • theoretical pI: 7.82963
  • theoretical MW: 62087.9
  • GRAVY: 0.1775

⊟Function[edit | edit source]

  • TIGRFAM:
    Unknown function General FTR1 family protein (TIGR00145; HMM-score: 89.8)
  • TheSEED  :
    • Ferrous iron transport permease EfeU
    Iron acquisition and metabolism Iron acquisition and metabolism - no subcategory Ferrous iron transporter EfeUOB, low-pH-induced  Ferrous iron transport permease EfeU
  • PFAM:
    MFS (CL0015) FTR1; Iron permease FTR1 family (PF03239; HMM-score: 71.1)
    and 3 more
    no clan defined Tim17; Tim17/Tim22/Tim23/Pmp24 family (PF02466; HMM-score: 15)
    MNHE; Na+/H+ ion antiporter subunit (PF01899; HMM-score: 9.8)
    CCDC84; Coiled coil protein 84 (PF14968; HMM-score: 6.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 6
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0
    • Signal peptide possibility: 0
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.466602
    • TAT(Tat/SPI): 0.005883
    • LIPO(Sec/SPII): 0.030313
  • predicted transmembrane helices (TMHMM): 6

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MLITAAMVCSFGLLKSQAAEQQSISDVYSVITDAKSALSNNSISNDNKQKAIEQVVSAVKKLSLEDNSESNAVKSDVRKLEDAKANDNQKDTLSQLTKSLIAYEEKLASKDAGSKIKLLQQQVDAKDAAMTKAIKDKNKAELESLNNSLNQIWTSNETVIRNYDANQYGQIEVALLQLRIAIHKSPLDTAKVSHAWTTFKSNIDHVDKKSNTSANDQYHVSQLNDALEKAIKAIDDNQLSDADAALTHFIETWPYVEGQIQTKDGALYTKIEDKIPYYQSVLDEHNKAHVKDGLVDLNNQIKEVVGHSYSFVDVMIIFLREGLEVLLIVMTLTTMTRNVKDKKGTASVIGGAIAGLVLSIILAITFVETLGNSGILRESMEAGLGIVAVILMFIVGVWMHKRSNAKRWNDMIKNMYANAISNGNLVLLATIGLISVLREGVEVIIFYMGMIGELATKDFIIGIALAIVILIIFALLFRFIVKLIPIFYIFRVLSIFIFIMGFKMLGVSIQKLQLLGAMPRHVIEGFPTINWLGFYPSYEPLIAQGAYIMVVAILIFKFKK

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: Fur* (repression) regulon
    Fur*(TF)important in Iron homeostasis; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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