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NCBI: 26-AUG-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus N315
- locus tag: SA0333 [new locus tag: SA_RS01910 ]
- pan locus tag?: SAUPAN001884000
- symbol: SA0333
- pan gene symbol?: fepC
- synonym:
- product: hypothetical protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SA0333 [new locus tag: SA_RS01910 ]
- symbol: SA0333
- product: hypothetical protein
- replicon: chromosome
- strand: +
- coordinates: 391022..392737
- length: 1716
- essential: no DEG other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 1123112 NCBI
- RefSeq: NP_373579 NCBI
- BioCyc: see SA_RS01910
- MicrobesOnline: 102605 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1681TTGGTGAAACATTATTTGACTAAATTTGTAGCAATGCTAATAACTGCTGCTATGGTGTGT
AGCTTTGGGTTACTGAAAAGTCAGGCAGCAGAACAACAAAGTATTAGTGATGTATATAGT
GTGATAACGGATGCGAAATCTGCACTTTCTAATAATTCGATATCGAATGACAATAAGCAG
AAAGCAATTGAGCAAGTGGTAAGTGCAGTTAAGAAATTATCGCTTGAAGATAATAGTGAA
GGTAATGCTGTCAAATCAGATGTGAGAAAGCTTGAAGATGCAAAAGCGAATGATAATCAA
AAAGATACACTTTCGCAATTAACGAAGTCATTAATTGCTTATGAAGAGAAATTGGCTAGT
AAAGATGCGGGTTCTAAAATTAAACTATTGCAACAGCAAGTCGATGCCAAAGATGCTGCG
ATGACAAAAGCGATTAAAGATAAAAATAAAGCGGAATTAGAATCTTTGAACAATAGTTTG
AATCAGATTTGGACAAGTAATGAAACAGTGATTCGCAATTATGACGCAAATCAATATGGA
CAAATTGAAGTCGCATTATTACAACTTAGAATTGCAATTCATAAGTCACCATTAGATACG
GCAAAAGTGTCACATGCTTGGACAACTTTTAAATCAAATATTGATCATGTCGATAAAAAA
AGTGATACGTCTGCAAATGATCAATACCATGTATCACAATTAAATGATGCGTTAGAGAAG
GCGATTAAAGCTATCGACGACAATCAATTGTCGGATGCTGATGCTGCGCTTACACATTTT
ATAGAAATTTGGCCGTATGTTGAAGGTCAAATTCAAACTAAAGACGGGGCTTTGTATACG
AAAATTGAAGATAAAATACCATATTATCAAAGTGTATTAGACGAACATAATAAATCGCAT
GTCAAAGATGGTTTAGTAGATATAAACAATCAAATTAAAGAGGTTGTTGGCCATAGTTAT
AGCTTTGTCGATGTGATGATTATCTTTTTACGTGAAGGACTAGAAGTGTTATTAATTGTA
ATGACATTGACTACCATGACGCGTAAAGTCAAAGATAATAAGGGGACTGCAAGTGTGATT
GGTGGTGCAATTGCCGGACTTGTACTGAGTATTATCTTAGCAATTACGTTTGTAGAAACT
TTAGGGAATAGTGGCATTCTTCGTGAAAGTATGGAAGCGGGATTAGGTATCGTTGCGGTC
ATATTAATGTTTATCGTTGGTGTTTGGATGCACAAACGTTCAAATGCGAAACGCTGGAAT
GATATGATTAAAAATATGTATGCCAATGCGATTAGTAATGGTAATTTGGTATTGTTAGCG
ACGATTGGTTTAATATCTGTGTTGCGTGAAGGTGTTGAGGTTATCATTTTCTATATGGGG
ATGATAGGTGAGCTAGCGACAAAAGATTTTATTATTGGTATTGCCTTGGCCATAGTGATA
TTAATTGTCTTTGCGGTATTATTTAGATTTATCGTCAGATTGATACCTATATTCTATATA
TTTAGAGTATTGTCGATCTTTATTTTTATTATGGGATTCAAAATGCTTGGCGTAAGTATT
CAAAAGTTACAATTATTAGGTGCGATGCCAAGACATGTTATTGAAGGATTCCCAACGATT
AACTGGTTGGGCTTCTATCCAAGTTATGAACCATTGATAGCACAAGCTGCTTATATTATG
GTAGTTGCTATCTTAATCTTTAAATTTAAAAAATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SA0333 [new locus tag: SA_RS01910 ]
- symbol: SA0333
- description: hypothetical protein
- length: 571
- theoretical pI: 8.31642
- theoretical MW: 63419.5
- GRAVY: 0.19352
⊟Function[edit | edit source]
- TIGRFAM: Unknown function General FTR1 family protein (TIGR00145; HMM-score: 89.4)
- TheSEED :
- Ferrous iron transport permease EfeU
- PFAM: MFS (CL0015) FTR1; Iron permease FTR1 family (PF03239; HMM-score: 72.4)and 5 moreno clan defined Tim17; Tim17/Tim22/Tim23/Pmp24 family (PF02466; HMM-score: 15)MNHE; Na+/H+ ion antiporter subunit (PF01899; HMM-score: 10.4)RecG_N; RecG N-terminal helical domain (PF17190; HMM-score: 9.5)DUF1539; Domain of Unknown Function (DUF1539) (PF07560; HMM-score: 8.3)CCDC84; Coiled coil protein 84 (PF14968; HMM-score: 6.5)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 7
- LocateP: Multi-transmembrane
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0
- Signal peptide possibility: 1
- N-terminally Anchored Score: -1
- Predicted Cleavage Site: No CleavageSite
- SignalP: Signal peptide SP(Sec/SPI) length 29 aa
- SP(Sec/SPI): 0.973397
- TAT(Tat/SPI): 0.005738
- LIPO(Sec/SPII): 0.017521
- Cleavage Site: CS pos: 29-30. SQA-AE. Pr: 0.9337
- predicted transmembrane helices (TMHMM): 7
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MVKHYLTKFVAMLITAAMVCSFGLLKSQAAEQQSISDVYSVITDAKSALSNNSISNDNKQKAIEQVVSAVKKLSLEDNSEGNAVKSDVRKLEDAKANDNQKDTLSQLTKSLIAYEEKLASKDAGSKIKLLQQQVDAKDAAMTKAIKDKNKAELESLNNSLNQIWTSNETVIRNYDANQYGQIEVALLQLRIAIHKSPLDTAKVSHAWTTFKSNIDHVDKKSDTSANDQYHVSQLNDALEKAIKAIDDNQLSDADAALTHFIEIWPYVEGQIQTKDGALYTKIEDKIPYYQSVLDEHNKSHVKDGLVDINNQIKEVVGHSYSFVDVMIIFLREGLEVLLIVMTLTTMTRKVKDNKGTASVIGGAIAGLVLSIILAITFVETLGNSGILRESMEAGLGIVAVILMFIVGVWMHKRSNAKRWNDMIKNMYANAISNGNLVLLATIGLISVLREGVEVIIFYMGMIGELATKDFIIGIALAIVILIVFAVLFRFIVRLIPIFYIFRVLSIFIFIMGFKMLGVSIQKLQLLGAMPRHVIEGFPTINWLGFYPSYEPLIAQAAYIMVVAILIFKFKK
⊟Experimental data[edit | edit source]
- experimentally validated:
- protein localization: data available for COL
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: SA0331 > SA0332 > SA0333
⊟Regulation[edit | edit source]
- regulator: Fur* (repression) regulon
Fur* (TF) important in Iron homeostasis; RegPrecise
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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