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NCBI: 26-AUG-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0333 [new locus tag: SA_RS01910 ]
  • pan locus tag?: SAUPAN001884000
  • symbol: SA0333
  • pan gene symbol?: fepC
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA0333 [new locus tag: SA_RS01910 ]
  • symbol: SA0333
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 391022..392737
  • length: 1716
  • essential: no DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    TTGGTGAAACATTATTTGACTAAATTTGTAGCAATGCTAATAACTGCTGCTATGGTGTGT
    AGCTTTGGGTTACTGAAAAGTCAGGCAGCAGAACAACAAAGTATTAGTGATGTATATAGT
    GTGATAACGGATGCGAAATCTGCACTTTCTAATAATTCGATATCGAATGACAATAAGCAG
    AAAGCAATTGAGCAAGTGGTAAGTGCAGTTAAGAAATTATCGCTTGAAGATAATAGTGAA
    GGTAATGCTGTCAAATCAGATGTGAGAAAGCTTGAAGATGCAAAAGCGAATGATAATCAA
    AAAGATACACTTTCGCAATTAACGAAGTCATTAATTGCTTATGAAGAGAAATTGGCTAGT
    AAAGATGCGGGTTCTAAAATTAAACTATTGCAACAGCAAGTCGATGCCAAAGATGCTGCG
    ATGACAAAAGCGATTAAAGATAAAAATAAAGCGGAATTAGAATCTTTGAACAATAGTTTG
    AATCAGATTTGGACAAGTAATGAAACAGTGATTCGCAATTATGACGCAAATCAATATGGA
    CAAATTGAAGTCGCATTATTACAACTTAGAATTGCAATTCATAAGTCACCATTAGATACG
    GCAAAAGTGTCACATGCTTGGACAACTTTTAAATCAAATATTGATCATGTCGATAAAAAA
    AGTGATACGTCTGCAAATGATCAATACCATGTATCACAATTAAATGATGCGTTAGAGAAG
    GCGATTAAAGCTATCGACGACAATCAATTGTCGGATGCTGATGCTGCGCTTACACATTTT
    ATAGAAATTTGGCCGTATGTTGAAGGTCAAATTCAAACTAAAGACGGGGCTTTGTATACG
    AAAATTGAAGATAAAATACCATATTATCAAAGTGTATTAGACGAACATAATAAATCGCAT
    GTCAAAGATGGTTTAGTAGATATAAACAATCAAATTAAAGAGGTTGTTGGCCATAGTTAT
    AGCTTTGTCGATGTGATGATTATCTTTTTACGTGAAGGACTAGAAGTGTTATTAATTGTA
    ATGACATTGACTACCATGACGCGTAAAGTCAAAGATAATAAGGGGACTGCAAGTGTGATT
    GGTGGTGCAATTGCCGGACTTGTACTGAGTATTATCTTAGCAATTACGTTTGTAGAAACT
    TTAGGGAATAGTGGCATTCTTCGTGAAAGTATGGAAGCGGGATTAGGTATCGTTGCGGTC
    ATATTAATGTTTATCGTTGGTGTTTGGATGCACAAACGTTCAAATGCGAAACGCTGGAAT
    GATATGATTAAAAATATGTATGCCAATGCGATTAGTAATGGTAATTTGGTATTGTTAGCG
    ACGATTGGTTTAATATCTGTGTTGCGTGAAGGTGTTGAGGTTATCATTTTCTATATGGGG
    ATGATAGGTGAGCTAGCGACAAAAGATTTTATTATTGGTATTGCCTTGGCCATAGTGATA
    TTAATTGTCTTTGCGGTATTATTTAGATTTATCGTCAGATTGATACCTATATTCTATATA
    TTTAGAGTATTGTCGATCTTTATTTTTATTATGGGATTCAAAATGCTTGGCGTAAGTATT
    CAAAAGTTACAATTATTAGGTGCGATGCCAAGACATGTTATTGAAGGATTCCCAACGATT
    AACTGGTTGGGCTTCTATCCAAGTTATGAACCATTGATAGCACAAGCTGCTTATATTATG
    GTAGTTGCTATCTTAATCTTTAAATTTAAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1716

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA0333 [new locus tag: SA_RS01910 ]
  • symbol: SA0333
  • description: hypothetical protein
  • length: 571
  • theoretical pI: 8.31642
  • theoretical MW: 63419.5
  • GRAVY: 0.19352

Function[edit | edit source]

  • TIGRFAM:
    Unknown function General FTR1 family protein (TIGR00145; HMM-score: 89.4)
  • TheSEED  :
    • Ferrous iron transport permease EfeU
    Iron acquisition and metabolism Iron acquisition and metabolism - no subcategory Ferrous iron transporter EfeUOB, low-pH-induced  Ferrous iron transport permease EfeU
  • PFAM:
    MFS (CL0015) FTR1; Iron permease FTR1 family (PF03239; HMM-score: 72.4)
    and 5 more
    no clan defined Tim17; Tim17/Tim22/Tim23/Pmp24 family (PF02466; HMM-score: 15)
    MNHE; Na+/H+ ion antiporter subunit (PF01899; HMM-score: 10.4)
    RecG_N; RecG N-terminal helical domain (PF17190; HMM-score: 9.5)
    DUF1539; Domain of Unknown Function (DUF1539) (PF07560; HMM-score: 8.3)
    CCDC84; Coiled coil protein 84 (PF14968; HMM-score: 6.5)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 7
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: Signal peptide SP(Sec/SPI) length 29 aa
    • SP(Sec/SPI): 0.973397
    • TAT(Tat/SPI): 0.005738
    • LIPO(Sec/SPII): 0.017521
    • Cleavage Site: CS pos: 29-30. SQA-AE. Pr: 0.9337
  • predicted transmembrane helices (TMHMM): 7

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MVKHYLTKFVAMLITAAMVCSFGLLKSQAAEQQSISDVYSVITDAKSALSNNSISNDNKQKAIEQVVSAVKKLSLEDNSEGNAVKSDVRKLEDAKANDNQKDTLSQLTKSLIAYEEKLASKDAGSKIKLLQQQVDAKDAAMTKAIKDKNKAELESLNNSLNQIWTSNETVIRNYDANQYGQIEVALLQLRIAIHKSPLDTAKVSHAWTTFKSNIDHVDKKSDTSANDQYHVSQLNDALEKAIKAIDDNQLSDADAALTHFIEIWPYVEGQIQTKDGALYTKIEDKIPYYQSVLDEHNKSHVKDGLVDINNQIKEVVGHSYSFVDVMIIFLREGLEVLLIVMTLTTMTRKVKDNKGTASVIGGAIAGLVLSIILAITFVETLGNSGILRESMEAGLGIVAVILMFIVGVWMHKRSNAKRWNDMIKNMYANAISNGNLVLLATIGLISVLREGVEVIIFYMGMIGELATKDFIIGIALAIVILIVFAVLFRFIVRLIPIFYIFRVLSIFIFIMGFKMLGVSIQKLQLLGAMPRHVIEGFPTINWLGFYPSYEPLIAQAAYIMVVAILIFKFKK

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator: Fur* (repression) regulon
    Fur*(TF)important in Iron homeostasis; RegPrecise 

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]