From AureoWiki
Jump to navigation Jump to search

NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00434
  • pan locus tag?: SAUPAN002185000
  • symbol: SAOUHSC_00434
  • pan gene symbol?: gltC
  • synonym:
  • product: LysR family transcriptional regulator

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00434
  • symbol: SAOUHSC_00434
  • product: LysR family transcriptional regulator
  • replicon: chromosome
  • strand: -
  • coordinates: 432267..433151
  • length: 885
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGAAATTAAACAATTAAGATATTTTATCGAGGTCGCTAAAAGAGAACATATCTCCGAA
    ACAGCACTTGAACTAAACATTGCTCAATCAGCGATAAGCCGTCAAATTACATTATTAGAA
    CAAGAACTTAATGTGTCTTTATTTAAAAAGCAAGGACGCAATATTACGCTCACATCTGAA
    GGGAAATTATTATTTAATGAAGCTCTGCGTATTATTGAACATTTAGATAGCACGATTGAA
    CAATTTCAAAGCCATGGATTAACTAAAAATAAAAGTATCTACATTGGCTATGATGAGAGT
    GACGTATCCCACATGTTACTACCACTTATCCAAACGTTTCATTTACAAAATGATACACAT
    GTCATACCGAGCTTATTAGAACATGACACAATTATAAATAGCGTATTAAATGGGAATATA
    GACATTGGTTTCACGGAACTTACACCAGAAATACGTAAACACAAACAATTACATATGCTA
    CCTTTATTCGAAGAGCACTATCATTTATATGCGCCTTCAGACGATCCAATTACGATGGCG
    ACACACCCACCATTAATTCAGTTTGAGCATAGTCATATTTATTGCTTGGCACCATTTGCC
    GAAACTGTAAAAAAACAATTGCGCAAGATTACTAAATCAGATGTATACACAATCAGTAGC
    CAACCACTTGCGCAGTATCTCCTTCGTCAAAAAGAAGGTTACATCATTTCATCACAAAAT
    ATACATTTACCAGAATCTAAATCATGGATAGATATAAAATTAGATCATACTGAATTAAAA
    AGAACGATATGTGCTATTACAAAGGAGCCTTATACAAAGAGTGATATTGGCATATTATTA
    ACACTTATTCAGCAATTAATGACTAAAACGTCTACATTCCATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    885

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_00434
  • symbol: SAOUHSC_00434
  • description: LysR family transcriptional regulator
  • length: 294
  • theoretical pI: 6.76432
  • theoretical MW: 33942.8
  • GRAVY: -0.257823

⊟Function[edit | edit source]

  • TIGRFAM:
    putative choline sulfate-utilization transcription factor (TIGR03418; HMM-score: 66.5)
    Metabolism Energy metabolism Other pca operon transcription factor PcaQ (TIGR02424; HMM-score: 59.3)
    Signal transduction Regulatory functions DNA interactions pca operon transcription factor PcaQ (TIGR02424; HMM-score: 59.3)
    and 5 more
    Signal transduction Regulatory functions DNA interactions aminoethylphosphonate catabolism associated LysR family transcriptional regulator (TIGR03339; HMM-score: 50.6)
    Signal transduction Regulatory functions DNA interactions D-serine deaminase transcriptional activator (TIGR02036; HMM-score: 40.5)
    Cellular processes Cellular processes Toxin production and resistance transcriptional regulator, ArgP family (TIGR03298; HMM-score: 29.2)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair transcriptional regulator, ArgP family (TIGR03298; HMM-score: 29.2)
    Signal transduction Regulatory functions DNA interactions transcriptional regulator, ArgP family (TIGR03298; HMM-score: 29.2)
  • TheSEED  :
    • GltC, transcription activator of glutamate synthase operon
    Regulation and Cell signaling Regulation and Cell signaling - no subcategory DNA-binding regulatory proteins, strays  GltC, transcription activator of glutamate synthase operon
  • PFAM:
    HTH (CL0123) HTH_1; Bacterial regulatory helix-turn-helix protein, lysR family (PF00126; HMM-score: 64.2)
    and 4 more
    PBP (CL0177) LysR_substrate; LysR substrate binding domain (PF03466; HMM-score: 33.7)
    no clan defined Ribosomal_S13_N; Ribosomal S13/S15 N-terminal domain (PF08069; HMM-score: 17.2)
    HTH (CL0123) HTH_34; Winged helix DNA-binding domain (PF13601; HMM-score: 16.3)
    HTH_5; Bacterial regulatory protein, arsR family (PF01022; HMM-score: 12.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors: 2-oxoglutarate; Glutamate
  • genes regulated by GltC*, TF important in Glutamate metabolismRegPrecise
    activation
    gltC*
    gltB* > gltD
    transcription units transferred from N315 data RegPrecise

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002278
    • TAT(Tat/SPI): 0.000185
    • LIPO(Sec/SPII): 0.000318
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MEIKQLRYFIEVAKREHISETALELNIAQSAISRQITLLEQELNVSLFKKQGRNITLTSEGKLLFNEALRIIEHLDSTIEQFQSHGLTKNKSIYIGYDESDVSHMLLPLIQTFHLQNDTHVIPSLLEHDTIINSVLNGNIDIGFTELTPEIRKHKQLHMLPLFEEHYHLYAPSDDPITMATHPPLIQFEHSHIYCLAPFAETVKKQLRKITKSDVYTISSQPLAQYLLRQKEGYIISSQNIHLPESKSWIDIKLDHTELKRTICAITKEPYTKSDIGILLTLIQQLMTKTSTFH

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: GltC* (activation) regulon, CodY* (repression) regulon
    GltC*(TF)important in Glutamate metabolism; RegPrecise 
    CodY*(TF)important in Amino acid metabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SAOUHSC_00434&oldid=79296"