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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00628
  • pan locus tag?: SAUPAN002497000
  • symbol: SAOUHSC_00628
  • pan gene symbol?: mnhD2
  • synonym:
  • product: monovalent cation/H+ antiporter subunit D

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00628
  • symbol: SAOUHSC_00628
  • product: monovalent cation/H+ antiporter subunit D
  • replicon: chromosome
  • strand: +
  • coordinates: 618074..619570
  • length: 1497
  • essential: no [1] DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGCTAAGTAACTTATTGATTTTACCAATGTTATTACCATTCCTTTGTGCCTTAATCCTT
    GTATTTTTAAAAAATAATGATCGTATTTCTAAATATTTATACTTAGGTACAATGACTATC
    ACCACAATTATTTCATTAATGCTATTAATTTATGTTCAGCGTCACCGTCCAATTACGCTA
    GACTTTGGAGGATGGTCAGCGCCCTTTGGTATACAGTTTTTAGGAGATTCTTTAAGTTTA
    ATTATGGTTACAACCGCTTCGTTTGTGATTACTTTAATTATGGCATACGGATTTGGGCGT
    GGCGAACATAAAGCAAATCGTTATCACTTGCCATCGTTCATATTATTTTTAAGTGTTGGC
    GTGATAGGCTCTTTTCTAACATCAGATTTATTTAATTTATACGTCATGTTTGAAATTATG
    TTACTAGCGTCATTTGTACTCATTACACTTGGACAATCTGTAGAACAATTACGTGCTGCA
    ATTATTTATGTTGTCTTGAATATTATTGGTTCATGGCTATTCTTATTAGGTATAGGTTTA
    CTTTATAAAACAGTAGGTACATTAAACTTTTCACATATTGCAATGCGTTTGAATGACATG
    GGAGATAATCGCACTGTTACAATGATTTCATTAATCTTCTTAGTCGCATTTAGTGCGAAA
    GCAGCGCTGGTCCTTTTTATGTGGCTACCCAAAGCCTACGCTGTGTTAAATACTGAGCTT
    GCAGCATTATTTGCAGCGTTAATGACCAAAGTAGGGGCCTATGCATTAATTCGATTCTTC
    ACTTTACTATTTGATCAACATAATGATCTCATACATCCATTGCTAGCAACTATGGCTGCT
    ATAACTATGGTCATCGGCGCTATAGGTGTCATTGCTTATAAAGATATTAAAAAGATTGCA
    GCTTACCAAGTCATAATCTCAATAGGATTTATCATTTTAGGTTTAGGAACAAACACGTTT
    GCAGGTATTAATGGTGCAATATTTTATTTGGTAAATGACATTGTTGTAAAAACATTGCTA
    TTTTTTATTATTGGTAGTTTAGTTTACATTACAGGCTATCGACAATATCAATATTTGAAT
    GGCTTAGCTAAAAAAGAACCTTTATTTGGAGTTGCGTTTATTATAATGATTTTTGCTATT
    GGCGGCGTGCCTCCATTTAGTGGCTTTCCGGGGAAAGTACTTATTTTCCAAGGTGCATTG
    CAAAATGGCAATTATATTGGACTAGCGTTAATGATTATTACTAGTCTAATTGCAATGTAC
    AGTTTATTTAGGATACTTTTTTATATGTATTTTGGAGATAAAGATGGGGAGGAAGTTAAT
    TTTAAGAAAATCCCGCTATATCGAAAAAGAATTTTAAGTATTTTAGTAGTTGTGGTTATC
    GCAATCGGAATTGCTGCACCTGTTGTGTTAAATGTTACAAGTGATGCAACTGAGTTGAAC
    ACGAGTGATCAATTATATCAAAAACTTGTAAATCCGCATTTGAAAGGAGAGGACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1497

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_00628
  • symbol: SAOUHSC_00628
  • description: monovalent cation/H+ antiporter subunit D
  • length: 498
  • theoretical pI: 9.6341
  • theoretical MW: 55152.9
  • GRAVY: 0.92249

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds multicomponent K+:H+antiporter (TIGR00944; HMM-score: 364)
    and 5 more
    Metabolism Energy metabolism Electron transport proton-translocating NADH-quinone oxidoreductase, chain M (TIGR01972; HMM-score: 203.4)
    Metabolism Energy metabolism Electron transport proton-translocating NADH-quinone oxidoreductase, chain N (TIGR01770; HMM-score: 176)
    Metabolism Energy metabolism Electron transport proton-translocating NADH-quinone oxidoreductase, chain L (TIGR01974; EC 1.6.-.-; HMM-score: 131.1)
    Metabolism Transport and binding proteins Cations and iron carrying compounds monovalent cation:proton antiporter (TIGR00940; HMM-score: 93.2)
    NAD(P)H dehydrogenase, subunit NdhF3 family (TIGR01960; HMM-score: 64.4)
  • TheSEED  :
    • Na(+) H(+) antiporter subunit D (TC 2.A.63.1.3)
    Membrane Transport Uni- Sym- and Antiporters Sodium Hydrogen Antiporter  Na(+) H(+) antiporter subunit D (TC 2.A.63.1.3)
  • PFAM:
    ComplexI-N (CL0425) Proton_antipo_M; Proton-conducting membrane transporter (PF00361; HMM-score: 189.5)
    and 3 more
    no clan defined Proton_antipo_N; NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminus (PF00662; HMM-score: 17.5)
    DUF1490; Protein of unknown function (DUF1490) (PF07371; HMM-score: 14)
    DUF2749; Protein of unknown function (DUF2749) (PF10907; HMM-score: 10.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 14
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00738
    • TAT(Tat/SPI): 0.000676
    • LIPO(Sec/SPII): 0.039209
  • predicted transmembrane helices (TMHMM): 14

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MLSNLLILPMLLPFLCALILVFLKNNDRISKYLYLGTMTITTIISLMLLIYVQRHRPITLDFGGWSAPFGIQFLGDSLSLIMVTTASFVITLIMAYGFGRGEHKANRYHLPSFILFLSVGVIGSFLTSDLFNLYVMFEIMLLASFVLITLGQSVEQLRAAIIYVVLNIIGSWLFLLGIGLLYKTVGTLNFSHIAMRLNDMGDNRTVTMISLIFLVAFSAKAALVLFMWLPKAYAVLNTELAALFAALMTKVGAYALIRFFTLLFDQHNDLIHPLLATMAAITMVIGAIGVIAYKDIKKIAAYQVIISIGFIILGLGTNTFAGINGAIFYLVNDIVVKTLLFFIIGSLVYITGYRQYQYLNGLAKKEPLFGVAFIIMIFAIGGVPPFSGFPGKVLIFQGALQNGNYIGLALMIITSLIAMYSLFRILFYMYFGDKDGEEVNFKKIPLYRKRILSILVVVVIAIGIAAPVVLNVTSDATELNTSDQLYQKLVNPHLKGED

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [2] [3]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SigB* (activation) regulon
    SigB*(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation;  [5] [4]   other strains

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  4. ↑ 4.0 4.1 4.2 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)
  5. ↑ Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J Bacteriol: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

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