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NCBI: 03-AUG-2016

Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00781
  • pan locus tag?: SAUPAN002679000
  • symbol: SAOUHSC_00781
  • pan gene symbol?: hprK
  • synonym:
  • product: HPr kinase/phosphorylase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00781
  • symbol: SAOUHSC_00781
  • product: HPr kinase/phosphorylase
  • replicon: chromosome
  • strand: +
  • coordinates: 763170..764102
  • length: 933
  • essential: yes [1] DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGTTAACGACAGAAAAACTAGTTGAAACATTAAAGTTAGATTTAATCGCTGGTGAAGAA
    GGACTATCGAAGCCAATTAAAAATGCTGATATATCAAGACCGGGCTTAGAGATGGCAGGT
    TATTTTTCACATTATGCGTCAGATAGAATACAACTATTAGGAACAACGGAACTATCGTTT
    TACAATTTATTACCAGATAAGGATCGCGCAGGTCGTATGCGTAAACTATGCAGACCAGAA
    ACGCCTGCAATTATTGTGACACGTGGATTGCAGCCACCAGAAGAATTAGTTGAAGCTGCA
    AAAGAATTAAATACCCCACTTATAGTTGCTAAAGATGCGACTACAAGTTTAATGAGTCGC
    TTAACAACGTTTTTAGAGCATGCACTTGCAAAGACGACATCTTTACATGGTGTTTTAGTA
    GATGTTTACGGTGTTGGTGTACTAATTACCGGTGATTCAGGAATAGGTAAAAGTGAGACT
    GCGTTGGAATTAGTTAAACGTGGGCATAGATTAGTAGCAGATGATAATGTAGAAATACGT
    CAAATTAATAAAGATGAACTAATAGGGAAACCACCAAAGTTAATAGAACATCTATTAGAA
    ATACGTGGACTAGGTATTATCAATGTTATGACTTTATTTGGCGCGGGTTCAATATTAACT
    GAAAAACGAATTAGATTAAATATTAATTTGGAAAACTGGAACAAGCAAAAGTTATATGAC
    CGCGTAGGTCTTAATGAAGAGACGCTAAGTATTTTAGATACTGAAATCACTAAAAAAACA
    ATACCTGTAAGACCTGGTAGAAATGTTGCGGTAATTATTGAGGTCGCTGCAATGAACTAT
    CGATTAAATATCATGGGCATTAACACGGCCGAAGAATTTAGTGAAAGATTAAATGAAGAA
    ATTATCAAGAACAGTCATAAGAGTGAGGAGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    933

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAOUHSC_00781
  • symbol: SAOUHSC_00781
  • description: HPr kinase/phosphorylase
  • length: 310
  • theoretical pI: 6.27773
  • theoretical MW: 34481.7
  • GRAVY: -0.173226

Function[edit | edit source]

  • reaction:
    EC 2.7.4.-?  ExPASy    EC 2.7.11.-?  ExPASy
    ATP + HPr = ADP + P-Ser-HPr? P-Ser-HPr + phosphate = HPr + diphosphate?
  • TIGRFAM:
    Signal transduction Regulatory functions Protein interactions HPr(Ser) kinase/phosphatase (TIGR00679; EC 2.7.1.-; HMM-score: 388)
    Signal transduction Signal transduction PTS HPr(Ser) kinase/phosphatase (TIGR00679; EC 2.7.1.-; HMM-score: 388)
    and 3 more
    HprK-related kinase B (TIGR04355; HMM-score: 23.5)
    HprK-related kinase A (TIGR04352; HMM-score: 15)
    SynChlorMet cassette protein ScmC (TIGR04249; HMM-score: 12.6)
  • TheSEED:  
    Carbohydrates Sugar alcohols Mannitol Utilization  HPr kinase/phosphorylase (EC 2.7.1.-) (EC 2.7.4.-)
    and 1 more
    Regulation and Cell signaling Regulation and Cell signaling - no subcategory HPr catabolite repression system  HPr kinase/phosphorylase (EC 2.7.1.-) (EC 2.7.4.-)
  • PFAM:
    PEP-carboxyk (CL0374) Hpr_kinase_C; HPr Serine kinase C-terminal domain (PF07475; HMM-score: 251.6)
    and 5 more
    MurF-HprK_N (CL0365) Hpr_kinase_N; HPr Serine kinase N terminus (PF02603; HMM-score: 134.3)
    no clan defined PucR; Purine catabolism regulatory protein-like family (PF07905; HMM-score: 26.7)
    MurF-HprK_N (CL0365) DRTGG; DRTGG domain (PF07085; HMM-score: 21)
    P-loop_NTPase (CL0023) AAA_16; AAA ATPase domain (PF13191; HMM-score: 13.3)
    AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 12.4)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00494
    • TAT(Tat/SPI): 0.000283
    • LIPO(Sec/SPII): 0.000768
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MLTTEKLVETLKLDLIAGEEGLSKPIKNADISRPGLEMAGYFSHYASDRIQLLGTTELSFYNLLPDKDRAGRMRKLCRPETPAIIVTRGLQPPEELVEAAKELNTPLIVAKDATTSLMSRLTTFLEHALAKTTSLHGVLVDVYGVGVLITGDSGIGKSETALELVKRGHRLVADDNVEIRQINKDELIGKPPKLIEHLLEIRGLGIINVMTLFGAGSILTEKRIRLNINLENWNKQKLYDRVGLNEETLSILDTEITKKTIPVRPGRNVAVIIEVAAMNYRLNIMGINTAEEFSERLNEEIIKNSHKSEE

Experimental data[edit | edit source]

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  4. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit | edit source]