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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00869
  • pan locus tag?: SAUPAN003031000
  • symbol: SAOUHSC_00869
  • pan gene symbol?: dltA
  • synonym:
  • product: D-alanine--poly(phosphoribitol) ligase subunit 1

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00869
  • symbol: SAOUHSC_00869
  • product: D-alanine--poly(phosphoribitol) ligase subunit 1
  • replicon: chromosome
  • strand: +
  • coordinates: 834275..835732
  • length: 1458
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGACAGATATTATTAACAAGCTGCAAGCGTTTGCGGATGCAAATCCACAAAGCATTGCT
    GTTAGACACACAACTGATGAATTAACTTATCAACAGTTAATGGATGAGTCTAGTAAATTA
    GCACATCGATTACAAGGTAGTAAGAAACCGATGATTTTATTCGGTCACATGTCACCATAT
    ATGATTGTTGGGATGATTGGTGCCATTAAAGCAGGATGTGGATATGTACCTGTAGACACT
    TCAATTCCTGAAGACCGTATTAAAATGATTATTAACAAGGTTCAACCAGAGTTTGTATTT
    AATACGACTGATGAATCATTTGAAAGTTTAGAAGGCGAAGTATTTACAATAGAAGATATT
    AAAACATCTCAAGACCCAGTAATTTTTGATAGTCAGATTAAAGATAACGACACAGTATAC
    ACAATCTTTACATCTGGTTCTACTGGGGAACCTAAAGGTGTTCAAATTGAATATGCAAGT
    TTAGTTCAATTTACTGAGTGGATGTTAGAACTTAATAAATCAGGAAATGAACAACAATGG
    CTTAACCAAGCGCCATTTTCATTTGATTTATCTGTAATGGCTATTTATCCATGTTTAGCA
    TCAGGCGGTACATTAAATCTTGTAGATAAAAACATGATTAATAAACCTAAATTATTAAAT
    GAAATGCTAACAGCAACACCGATTAACATTTGGGTATCAACACCATCATTTATGGAAATG
    TGTTTATTATTACCAACGCTTAATGAAGAACAATATGGTAGTCTTAACGAATTCTTCTTC
    TGTGGTGAAATTCTACCTCACAGAGCAGCAAAAGCGTTAGTAAACCGTTTCCCAAGTGCG
    ACGATTTACAACACATATGGTCCAACTGAAGCTACGGTAGCAGTTACAAGTATTCAAATT
    ACACAAGAAATCTTAGATCAATATCCGACATTACCTGTTGGCGTTGAAAGACCAGGCGCA
    AGATTATCTACTACAGATGAAGGTGAACTTGTTATCGAAGGTCAAAGTGTAAGTTTAGGA
    TACTTAAAAAATGACCAAAAAACAGCTGAAGTATTTAATTTCGATGACGGTATTCGTACA
    TATCACACTGGTGATAAAGCGAAGTTTGAAAATGGTCAATGGTTCATTCAAGGTCGTATT
    GATTTCCAAATCAAATTGAATGGCTACAGAATGGAATTAGAAGAAATTGAAACACAATTA
    CGCCAGTCTGAGTTCGTAAAAGAAGCGATTGTTGTACCTGTATATAAAAATGATAAAGTT
    ATTCATTTAATTGGTGCAATTGTGCCAACGACTGAAGTTACGGACAATGCAGAAATGACT
    AAAAATATTAAAAATGACTTGAAATCACGCTTACCAGAGTATATGATTCCTAGAAAGTTT
    GAATGGATGGAACAATTGCCATTGACTTCAAATGGTAAAATTGACAGAAAGAAAATTGCA
    GAGGTAATTAACGGATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1458

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_00869
  • symbol: SAOUHSC_00869
  • description: D-alanine--poly(phosphoribitol) ligase subunit 1
  • length: 485
  • theoretical pI: 4.55305
  • theoretical MW: 54670.1
  • GRAVY: -0.251959

⊟Function[edit | edit source]

  • reaction:
    EC 6.1.1.13?  ExPASy
    D-alanine--poly(phosphoribitol) ligase ATP + D-alanine + poly(ribitol phosphate) = AMP + diphosphate + O-D-alanyl-poly(ribitol phosphate)
    EC 6.2.1.-?  ExPASy
    D-alanine + ATP + holo-[D-alanyl-carrier protein] = AMP + diphosphate + D-alanyl-[D-alanyl-carrier protein]?
  • TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan D-alanine--poly(phosphoribitol) ligase, subunit 1 (TIGR01734; EC 6.1.1.13; HMM-score: 755.6)
    and 12 more
    amino acid adenylation domain (TIGR01733; HMM-score: 322.6)
    acyl-CoA ligase (AMP-forming), exosortase A-associated (TIGR03098; HMM-score: 160.7)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone O-succinylbenzoate-CoA ligase (TIGR01923; EC 6.2.1.26; HMM-score: 137.3)
    L-aminoadipate-semialdehyde dehydrogenase (TIGR03443; EC 1.2.1.31; HMM-score: 115.2)
    benzoate-CoA ligase family (TIGR02262; HMM-score: 100.1)
    acetate--CoA ligase (TIGR02188; EC 6.2.1.1; HMM-score: 85)
    Metabolism Transport and binding proteins Cations and iron carrying compounds (2,3-dihydroxybenzoyl)adenylate synthase (TIGR02275; EC 2.7.7.58; HMM-score: 76.9)
    cyclohexanecarboxylate-CoA ligase (TIGR03208; EC 6.2.1.-; HMM-score: 45.4)
    propionate--CoA ligase (TIGR02316; EC 6.2.1.17; HMM-score: 39.6)
    Metabolism Central intermediary metabolism Other acetoacetate-CoA ligase (TIGR01217; EC 6.2.1.16; HMM-score: 38.2)
    dicarboxylate--CoA ligase PimA (TIGR03205; EC 6.2.1.23; HMM-score: 35.4)
    4-coumarate--CoA ligase (TIGR02372; EC 6.2.1.12; HMM-score: 33.2)
  • TheSEED  :
    • D-alanine--poly(phosphoribitol) ligase subunit 1 (EC 6.1.1.13)
    Cell Wall and Capsule Gram-Positive cell wall components D-Alanyl Lipoteichoic Acid Biosynthesis  D-alanine--poly(phosphoribitol) ligase subunit 1 (EC 6.1.1.13)
    and 1 more
    Cell Wall and Capsule Gram-Positive cell wall components Teichoic and lipoteichoic acids biosynthesis  D-alanine--poly(phosphoribitol) ligase subunit 1 (EC 6.1.1.13)
  • PFAM:
    ANL (CL0378) AMP-binding; AMP-binding enzyme (PF00501; HMM-score: 243.5)
    and 2 more
    AMP-binding_C (CL0531) AMP-binding_C; AMP-binding enzyme C-terminal domain (PF13193; HMM-score: 42.4)
    no clan defined NIR_SIR; Nitrite and sulphite reductase 4Fe-4S domain (PF01077; HMM-score: 12.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004344
    • TAT(Tat/SPI): 0.000288
    • LIPO(Sec/SPII): 0.000819
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTDIINKLQAFADANPQSIAVRHTTDELTYQQLMDESSKLAHRLQGSKKPMILFGHMSPYMIVGMIGAIKAGCGYVPVDTSIPEDRIKMIINKVQPEFVFNTTDESFESLEGEVFTIEDIKTSQDPVIFDSQIKDNDTVYTIFTSGSTGEPKGVQIEYASLVQFTEWMLELNKSGNEQQWLNQAPFSFDLSVMAIYPCLASGGTLNLVDKNMINKPKLLNEMLTATPINIWVSTPSFMEMCLLLPTLNEEQYGSLNEFFFCGEILPHRAAKALVNRFPSATIYNTYGPTEATVAVTSIQITQEILDQYPTLPVGVERPGARLSTTDEGELVIEGQSVSLGYLKNDQKTAEVFNFDDGIRTYHTGDKAKFENGQWFIQGRIDFQIKLNGYRMELEEIETQLRQSEFVKEAIVVPVYKNDKVIHLIGAIVPTTEVTDNAEMTKNIKNDLKSRLPEYMIPRKFEWMEQLPLTSNGKIDRKKIAEVING

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [2] [3]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  4. ↑ 4.0 4.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

A Peschel, M Otto, R W Jack, H Kalbacher, G Jung, F Götz
Inactivation of the dlt operon in Staphylococcus aureus confers sensitivity to defensins, protegrins, and other antimicrobial peptides.
J Biol Chem: 1999, 274(13);8405-10
[PubMed:10085071] [WorldCat.org] [DOI] (P p)

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