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NCBI: 03-AUG-2016

Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00931
  • pan locus tag?: SAUPAN003152000
  • symbol: SAOUHSC_00931
  • pan gene symbol?: opp4B
  • synonym:
  • product: oligopeptide ABC transporter permease

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00931
  • symbol: SAOUHSC_00931
  • product: oligopeptide ABC transporter permease
  • replicon: chromosome
  • strand: +
  • coordinates: 903651..904613
  • length: 963
  • essential: no DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGATTAAATTAATACTTAAAAGATTAGGGTTAATGATCCCGTTACTAATTTTAATCTCA
    ATAGTTGTATTTTCATTAGCAATCATACAACCAGGAGATCCATTTTCAGATCTACAAAAC
    GGTAAAATAAAACAAGAAGCAATAAATGCACAAAGGGAAAAATTAGGTCTCAACGACTCA
    ATACCACATCAATACATTAGATGGGTCAATCATGTTATACACGGTGATTTAGGGGAGTCA
    ATCAAATATAAAAGGCCGGTAATTGATGTTATTGAGGAAAGAATTCCAAATACAATATTA
    CTCGGTGCTATGTCATTAATTATTACTTATATTATCTCATTTGCTTTAGGAATAACGTCA
    GGTAGATATTCTTACAGTTTGACGGATTATACTGTGCAAATATTTAATTATTTGATGTTA
    GCCATTCCATCTTTTATTGCGGGAGTATTTGCAATTTTTATTTTTTCTTTTGAATTACAA
    TGGTTCCCGTTTCAAGGTTCTGTTGATATTAACCTTAAAGAAGGTACTTTTGAATATTAT
    ATGAGTAAAATATATCACACATTTTTACCTGCATTCACTTTAGGTTTATTATCTACTGCT
    GGTTATATTCAATATTTACGTAATGATATTATTGAAAATTCTAAAAAAGATTATGTATTG
    ACGGCAAGGTCAAAAGGATTATCTATGAATAAAATTTATAATAAACATATATTGAGAAAT
    TCTCTAATACCTATTATTACATTTTTAGGTGCTGATATTGTAAGTATTTTAGGTGGAGCC
    GTGATTACGGAGACTATCTTTTCATATAACGGTATCGGTAAATTATTTTTAGAATCGGTA
    ATAGGCCAAGACTATCCATTAATGATGGCATTAACGTTATTTTTCTCATTTTTAGGTTTA
    CTGGGTAATTTGATTTCTGATATTACTTATGGATTTATAGATCCAAGAATTAGAAGTAAC
    TAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    963

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAOUHSC_00931
  • symbol: SAOUHSC_00931
  • description: oligopeptide ABC transporter permease
  • length: 320
  • theoretical pI: 8.94727
  • theoretical MW: 36123.3
  • GRAVY: 0.459062

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds nickel ABC transporter, permease subunit NikB (TIGR02789; EC 3.6.3.24; HMM-score: 236.8)
    and 3 more
    Metabolism Transport and binding proteins Cations and iron carrying compounds nickel ABC transporter, permease subunit NikC (TIGR02790; EC 3.6.3.24; HMM-score: 23.4)
    Metabolism Transport and binding proteins Anions phosphate ABC transporter, permease protein PstC (TIGR02138; HMM-score: 18)
    Metabolism Transport and binding proteins Anions phosphate ABC transporter, permease protein PstA (TIGR00974; HMM-score: 15.3)
  • TheSEED  :
    • Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1)
    Membrane Transport ABC transporters ABC transporter oligopeptide (TC 3.A.1.5.1)  Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1)
  • PFAM:
    BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 138.5)
    and 1 more
    IT (CL0182) CitMHS; Citrate transporter (PF03600; HMM-score: 11.2)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 6
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: Signal peptide SP(Sec/SPI) length 27 aa
    • SP(Sec/SPI): 0.517601
    • TAT(Tat/SPI): 0.001512
    • LIPO(Sec/SPII): 0.035073
    • Cleavage Site: CS pos: 27-28. SLA-II. Pr: 0.1516
  • predicted transmembrane helices (TMHMM): 6

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MIKLILKRLGLMIPLLILISIVVFSLAIIQPGDPFSDLQNGKIKQEAINAQREKLGLNDSIPHQYIRWVNHVIHGDLGESIKYKRPVIDVIEERIPNTILLGAMSLIITYIISFALGITSGRYSYSLTDYTVQIFNYLMLAIPSFIAGVFAIFIFSFELQWFPFQGSVDINLKEGTFEYYMSKIYHTFLPAFTLGLLSTAGYIQYLRNDIIENSKKDYVLTARSKGLSMNKIYNKHILRNSLIPIITFLGADIVSILGGAVITETIFSYNGIGKLFLESVIGQDYPLMMALTLFFSFLGLLGNLISDITYGFIDPRIRSN

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator: CodY* (repression) regulon
    CodY*(TF)important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit | edit source]