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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL0999 [new locus tag: SACOL_RS05105 ]
- pan locus tag?: SAUPAN003152000
- symbol: SACOL0999
- pan gene symbol?: opp4B
- synonym:
- product: oligopeptide ABC transporter permease
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL0999 [new locus tag: SACOL_RS05105 ]
- symbol: SACOL0999
- product: oligopeptide ABC transporter permease
- replicon: chromosome
- strand: +
- coordinates: 1007261..1008223
- length: 963
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3236083 NCBI
- RefSeq: YP_185867 NCBI
- BioCyc: see SACOL_RS05105
- MicrobesOnline: 912468 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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961ATGATTAAATTAATACTTAAAAGATTAGGGTTAATGATCCCGTTACTAATTTTAATCTCA
ATAGTTGTATTTTCATTAGCAATCATACAACCAGGAGATCCATTTTCAGATCTACAAAAC
GGTAAAATAAAACAAGAAGCAATAAATGCACAAAGGGAAAAATTAGGTCTCAACGACTCA
ATACCACATCAATACATTAGATGGGTCAATCATGTTATACACGGTGATTTAGGGGAGTCA
ATCAAATATAAAAGGCCGGTAATTGATGTTATTGAGGAAAGAATTCCAAATACAATATTA
CTCGGTGCTATGTCATTAATTATTACTTATATTATCTCATTTGCTTTAGGAATAACGTCA
GGTAGATATTCTTACAGTTTGACGGATTATACTGTGCAAATATTTAATTATTTGATGTTA
GCCATTCCATCTTTTATTGCGGGAGTATTTGCAATTTTTATTTTTTCTTTTGAATTACAA
TGGTTCCCGTTTCAAGGTTCTGTTGATATTAACCTTAAAGAAGGTACTTTTGAATATTAT
ATGAGTAAAATATATCACACATTTTTACCTGCATTCACTTTAGGTTTATTATCTACTGCT
GGTTATATTCAATATTTACGTAATGATATTATTGAAAATTCTAAAAAAGATTATGTATTG
ACGGCAAGGTCAAAAGGATTATCTATGAATAAAATTTATAATAAACATATATTGAGAAAT
TCTCTAATACCTATTATTACATTTTTAGGTGCTGATATTGTAAGTATTTTAGGTGGAGCC
GTGATTACGGAGACTATCTTTTCATATAACGGTATCGGTAAATTATTTTTAGAATCGGTA
ATAGGCCAAGACTATCCATTAATGATGGCATTAACGTTATTTTTCTCATTTTTAGGTTTA
CTGGGTAATTTGATTTCTGATATTACTTATGGATTTATAGATCCAAGAATTAGAAGTAAC
TAG60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL0999 [new locus tag: SACOL_RS05105 ]
- symbol: SACOL0999
- description: oligopeptide ABC transporter permease
- length: 320
- theoretical pI: 8.94727
- theoretical MW: 36123.3
- GRAVY: 0.459062
⊟Function[edit | edit source]
- TIGRFAM: Transport and binding proteins Cations and iron carrying compounds nickel ABC transporter, permease subunit NikB (TIGR02789; EC 3.6.3.24; HMM-score: 236.8)and 3 moreTransport and binding proteins Cations and iron carrying compounds nickel ABC transporter, permease subunit NikC (TIGR02790; EC 3.6.3.24; HMM-score: 23.4)Transport and binding proteins Anions phosphate ABC transporter, permease protein PstC (TIGR02138; HMM-score: 18)Transport and binding proteins Anions phosphate ABC transporter, permease protein PstA (TIGR00974; HMM-score: 15.3)
- TheSEED :
- Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1)
- PFAM: BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 138.5)and 1 moreIT (CL0182) CitMHS; Citrate transporter (PF03600; HMM-score: 11.2)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 6
- LocateP: Multi-transmembrane
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0.17
- Signal peptide possibility: 0.5
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: Signal peptide SP(Sec/SPI) length 27 aa
- SP(Sec/SPI): 0.517601
- TAT(Tat/SPI): 0.001512
- LIPO(Sec/SPII): 0.035073
- Cleavage Site: CS pos: 27-28. SLA-II. Pr: 0.1516
- predicted transmembrane helices (TMHMM): 6
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MIKLILKRLGLMIPLLILISIVVFSLAIIQPGDPFSDLQNGKIKQEAINAQREKLGLNDSIPHQYIRWVNHVIHGDLGESIKYKRPVIDVIEERIPNTILLGAMSLIITYIISFALGITSGRYSYSLTDYTVQIFNYLMLAIPSFIAGVFAIFIFSFELQWFPFQGSVDINLKEGTFEYYMSKIYHTFLPAFTLGLLSTAGYIQYLRNDIIENSKKDYVLTARSKGLSMNKIYNKHILRNSLIPIITFLGADIVSILGGAVITETIFSYNGIGKLFLESVIGQDYPLMMALTLFFSFLGLLGNLISDITYGFIDPRIRSN
⊟Experimental data[edit | edit source]
- experimentally validated:
- protein localization:
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: CodY (repression) regulon
CodY (TF) important in Amino acid metabolism; RegPrecise transcription unit transferred from N315 data RegPrecise
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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