From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0999 [new locus tag: SACOL_RS05105 ]
  • pan locus tag?: SAUPAN003152000
  • symbol: SACOL0999
  • pan gene symbol?: opp4B
  • synonym:
  • product: oligopeptide ABC transporter permease

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0999 [new locus tag: SACOL_RS05105 ]
  • symbol: SACOL0999
  • product: oligopeptide ABC transporter permease
  • replicon: chromosome
  • strand: +
  • coordinates: 1007261..1008223
  • length: 963
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGATTAAATTAATACTTAAAAGATTAGGGTTAATGATCCCGTTACTAATTTTAATCTCA
    ATAGTTGTATTTTCATTAGCAATCATACAACCAGGAGATCCATTTTCAGATCTACAAAAC
    GGTAAAATAAAACAAGAAGCAATAAATGCACAAAGGGAAAAATTAGGTCTCAACGACTCA
    ATACCACATCAATACATTAGATGGGTCAATCATGTTATACACGGTGATTTAGGGGAGTCA
    ATCAAATATAAAAGGCCGGTAATTGATGTTATTGAGGAAAGAATTCCAAATACAATATTA
    CTCGGTGCTATGTCATTAATTATTACTTATATTATCTCATTTGCTTTAGGAATAACGTCA
    GGTAGATATTCTTACAGTTTGACGGATTATACTGTGCAAATATTTAATTATTTGATGTTA
    GCCATTCCATCTTTTATTGCGGGAGTATTTGCAATTTTTATTTTTTCTTTTGAATTACAA
    TGGTTCCCGTTTCAAGGTTCTGTTGATATTAACCTTAAAGAAGGTACTTTTGAATATTAT
    ATGAGTAAAATATATCACACATTTTTACCTGCATTCACTTTAGGTTTATTATCTACTGCT
    GGTTATATTCAATATTTACGTAATGATATTATTGAAAATTCTAAAAAAGATTATGTATTG
    ACGGCAAGGTCAAAAGGATTATCTATGAATAAAATTTATAATAAACATATATTGAGAAAT
    TCTCTAATACCTATTATTACATTTTTAGGTGCTGATATTGTAAGTATTTTAGGTGGAGCC
    GTGATTACGGAGACTATCTTTTCATATAACGGTATCGGTAAATTATTTTTAGAATCGGTA
    ATAGGCCAAGACTATCCATTAATGATGGCATTAACGTTATTTTTCTCATTTTTAGGTTTA
    CTGGGTAATTTGATTTCTGATATTACTTATGGATTTATAGATCCAAGAATTAGAAGTAAC
    TAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    963

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL0999 [new locus tag: SACOL_RS05105 ]
  • symbol: SACOL0999
  • description: oligopeptide ABC transporter permease
  • length: 320
  • theoretical pI: 8.94727
  • theoretical MW: 36123.3
  • GRAVY: 0.459062

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds nickel ABC transporter, permease subunit NikB (TIGR02789; EC 3.6.3.24; HMM-score: 236.8)
    and 3 more
    Metabolism Transport and binding proteins Cations and iron carrying compounds nickel ABC transporter, permease subunit NikC (TIGR02790; EC 3.6.3.24; HMM-score: 23.4)
    Metabolism Transport and binding proteins Anions phosphate ABC transporter, permease protein PstC (TIGR02138; HMM-score: 18)
    Metabolism Transport and binding proteins Anions phosphate ABC transporter, permease protein PstA (TIGR00974; HMM-score: 15.3)
  • TheSEED  :
    • Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1)
    Membrane Transport ABC transporters ABC transporter oligopeptide (TC 3.A.1.5.1)  Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1)
  • PFAM:
    BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 138.5)
    and 1 more
    IT (CL0182) CitMHS; Citrate transporter (PF03600; HMM-score: 11.2)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 6
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: Signal peptide SP(Sec/SPI) length 27 aa
    • SP(Sec/SPI): 0.517601
    • TAT(Tat/SPI): 0.001512
    • LIPO(Sec/SPII): 0.035073
    • Cleavage Site: CS pos: 27-28. SLA-II. Pr: 0.1516
  • predicted transmembrane helices (TMHMM): 6

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MIKLILKRLGLMIPLLILISIVVFSLAIIQPGDPFSDLQNGKIKQEAINAQREKLGLNDSIPHQYIRWVNHVIHGDLGESIKYKRPVIDVIEERIPNTILLGAMSLIITYIISFALGITSGRYSYSLTDYTVQIFNYLMLAIPSFIAGVFAIFIFSFELQWFPFQGSVDINLKEGTFEYYMSKIYHTFLPAFTLGLLSTAGYIQYLRNDIIENSKKDYVLTARSKGLSMNKIYNKHILRNSLIPIITFLGADIVSILGGAVITETIFSYNGIGKLFLESVIGQDYPLMMALTLFFSFLGLLGNLISDITYGFIDPRIRSN

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator: CodY (repression) regulon
    CodY(TF)important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]