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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00994
  • pan locus tag?: SAUPAN003260000
  • symbol: SAOUHSC_00994
  • pan gene symbol?: atl
  • synonym: atlA
  • product: bifunctional autolysin

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00994
  • symbol: SAOUHSC_00994
  • product: bifunctional autolysin
  • replicon: chromosome
  • strand: -
  • coordinates: 964093..967863
  • length: 3771
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGAAAAAATTCAATTACAAACTACCATCAATGGTTGCATTAACGCTTGTAGGTTCA
    GCAGTCACTGCACATCAAGTTCAAGCAGCTGAGACGACACAAGATCAAACTACTAATAAA
    AACGTTTTAGATAGTAATAAAGTTAAAGCAACTACTGAACAAGCAAAAGCTGAGGTAAAA
    AATCCAACGCAAAACATTTCTGGCACTCAAGTATATCAAGACCCTGCTATTGTCCAACCA
    AAAACAGCAAATAACAAAACAGGCAATGCTCAAGTAAGTCAAAAAGTTGATACTGCACAA
    GTAAATGGTGACACTCGTGCTAATCAATCAGCGACTACAAATAATACGCAGCCTGTTGCA
    AAGTCAACAAGCACTACAGCACCTAAAACTAACACTAATGTTACAAATGCTGGTTATAGT
    TTAGTTGATGATGAAGATGATAATTCAGAAAATCAAATTAATCCAGAATTAATTAAATCA
    GCTGCTAAACCTGCAGCTCTTGAAACGCAATATAAAACCGCAGCACCTAAAGCTGCAACT
    ACATCAGCACCTAAAGCTAAAACTGAAGCGACACCTAAAGTAACTACTTTTAGCGCTTCA
    GCACAACCAAGATCAGTTGCTGCAACACCAAAAACGAGTTTGCCAAAATATAAACCACAA
    GTAAACTCTTCAATTAACGATTACATTTGTAAAAATAACTTAAAAGCACCTAAAATTGAA
    GAAGATTATACATCTTACTTCCCTAAATACGCATACCGTAACGGCGTAGGTCGTCCTGAA
    GGTATCGTAGTTCATGATACAGCTAATGATCGTTCGACGATAAATGGTGAAATTAGTTAT
    ATGAAAAATAACTATCAAAACGCATTCGTACATGCATTTGTTGATGGGGATCGTATAATC
    GAAACAGCACCAACGGATTACTTATCTTGGGGTGTCGGTGCAGTCGGTAACCCTAGATTC
    ATCAATGTTGAAATCGTACACACACACGACTATGCTTCATTTGCACGTTCAATGAATAAC
    TATGCTGACTATGCAGCTACACAATTACAATATTATGGTTTAAAACCAGACAGTGCTGAG
    TATGATGGAAATGGTACAGTATGGACTCACTACGCTGTAAGTAAATATTTAGGTGGTACT
    GACCATGCCGATCCACATGGATATTTAAGAAGTCATAATTATAGTTATGATCAATTATAT
    GACTTAATTAATGAAAAATATTTAATAAAAATGGGTAAAGTGGCGCCATGGGGTACGCAA
    TCTACAACTACCCCTACTACACCATCAAAACCAACAACACCGTCGAAACCATCAACTGGT
    AAATTAACAGTTGCTGCAAACAATGGTGTCGCACAAATCAAACCAACAAATAGTGGTTTA
    TATACTACTGTATACGACAAAACTGGTAAAGCAACTAATGAAGTTCAAAAAACATTTGCT
    GTATCTAAAACAGCTACATTAGGTAATCAAAAATTCTATCTTGTTCAAGATTACAATTCT
    GGTAATAAATTTGGTTGGGTTAAAGAAGGCGATGTGGTTTACAACACAGCTAAATCACCT
    GTAAATGTAAATCAATCATATTCAATCAAACCTGGTACGAAACTTTATACAGTACCTTGG
    GGTACATCTAAACAAGTTGCTGGTAGTGTGTCTGGCTCTGGAAACCAAACATTTAAGGCT
    TCAAAGCAACAACAAATTGATAAATCAATTTATTTATATGGCTCTGTGAATGGTAAATCT
    GGTTGGGTAAGTAAAGCATATTTAGTTGATACTGCTAAACCTACGCCTACACCAACACCT
    AAGCCATCAACACCTACAACAAATAATAAATTAACAGTTTCATCATTAAACGGTGTTGCT
    CAAATTAATGCTAAAAACAATGGCTTATTCACTACAGTTTATGACAAAACTGGTAAGCCA
    ACGAAAGAAGTTCAAAAAACATTTGCTGTAACAAAAGAAGCAAGTTTAGGTGGAAACAAA
    TTCTACTTAGTTAAAGATTACAATAGTCCAACTTTAATTGGTTGGGTTAAACAAGGTGAC
    GTTATTTATAACAATGCAAAATCACCTGTAAATGTAATGCAAACATATACAGTAAAACCA
    GGCACTAAATTATATTCAGTACCTTGGGGCACTTATAAACAAGAAGCTGGTGCAGTTTCT
    GGTACAGGTAACCAAACTTTTAAAGCGACTAAGCAACAACAAATTGATAAATCTATCTAT
    TTATTTGGAACTGTAAATGGTAAATCTGGTTGGGTAAGTAAAGCATATTTAGCTGTACCT
    GCTGCACCTAAAAAAGCAGTAGCACAACCAAAAACAGCTGTAAAAGCTTATACTGTTACT
    AAACCACAAACGACTCAAACAGTTAGCAAGATTGCTCAAGTTAAACCAAACAACACTGGT
    ATTCGTGCTTCTGTTTATGAAAAAACAGCGAAAAACGGTGCGAAATATGCAGACCGTACG
    TTCTATGTAACAAAAGAGCGTGCTCATGGTAATGAAACGTATGTATTATTAAACAATACA
    AGCCATAACATCCCATTAGGTTGGTTCAATGTAAAAGACTTAAATGTTCAAAACTTAGGC
    AAAGAAGTTAAAACGACTCAAAAATATACTGTTAATAAATCAAATAACGGCTTATCAATG
    GTTCCTTGGGGTACTAAAAACCAAGTCATTTTAACAGGCAATAACATTGCTCAAGGTACA
    TTTAATGCAACGAAACAAGTATCTGTAGGCAAAGATGTTTATTTATACGGTACTATTAAT
    AACCGCACTGGTTGGGTAAATGCAAAAGATTTAACTGCACCAACTGCTGTGAAACCAACT
    ACATCAGCTGCCAAAGATTATAACTACACTTATGTAATTAAAAATGGTAATGGTTATTAC
    TATGTAACACCAAATTCTGATACAGCTAAATACTCATTAAAAGCATTTAATGAACAACCA
    TTCGCAGTTGTTAAAGAACAAGTCATTAATGGACAAACTTGGTACTATGGTAAATTATCT
    AACGGTAAATTAGCATGGATTAAATCAACTGATTTAGCTAAAGAATTAATTAAGTATAAT
    CAAACAGGTATGACATTAAACCAAGTTGCTCAAATACAAGCTGGTTTACAATATAAACCA
    CAAGTACAACGTGTACCAGGTAAGTGGACAGATGCTAAATTTAATGATGTTAAGCATGCA
    ATGGATACGAAGCGTTTAGCTCAAGATCCAGCATTAAAATATCAATTCTTACGCTTAGAC
    CAACCACAAAATATTTCTATTGATAAAATTAATCAATTCTTAAAAGGTAAAGGTGTATTA
    GAAAACCAAGGTGCTGCATTTAACAAAGCTGCTCAAATGTATGGCATTAATGAAGTTTAT
    CTTATCTCACATGCCCTATTAGAAACAGGTAACGGTACTTCTCAATTAGCGAAAGGTGCA
    GATGTAGTGAACAACAAAGTTGTAACTAACTCAAACACGAAATACCATAACGTATTTGGT
    ATTGCTGCATATGATAACGATCCTTTACGTGAAGGTATTAAATATGCTAAACAAGCTGGT
    TGGGACACAGTATCAAAAGCAATCGTTGGTGGTGCTAAATTCATCGGCAACTCATATGTA
    AAAGCTGGTCAAAATACACTTTACAAAATGAGATGGAATCCTGCACATCCAGGAACACAC
    CAATATGCTACAGATGTAGATTGGGCTAACATCAATGCTAAAATCATCAAAGGCTACTAT
    GATAAAATTGGCGAAGTCGGCAAATACTTCGACATCCCACAATATAAATAA
    60
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    360
    420
    480
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    660
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    840
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    960
    1020
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    2160
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    3060
    3120
    3180
    3240
    3300
    3360
    3420
    3480
    3540
    3600
    3660
    3720
    3771

⊟Protein[edit | edit source]

Protein Data Bank: 4KNK
Protein Data Bank: 4KNL

⊟General[edit | edit source]

  • locus tag: SAOUHSC_00994
  • symbol: SAOUHSC_00994
  • description: bifunctional autolysin
  • length: 1256
  • theoretical pI: 10.0768
  • theoretical MW: 137383
  • GRAVY: -0.615366

⊟Function[edit | edit source]

  • reaction:
    EC 3.2.1.96?  ExPASy
    Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase Endohydrolysis of the N,N'-diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the -(Man(GlcNAc)2)Asn-structure. One N-acetyl-D-glucosamine residue remains attached to the protein; the rest of the oligosaccharide is released intact
    EC 3.5.1.28?  ExPASy
    N-acetylmuramoyl-L-alanine amidase Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides
  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds V-type ATPase, D subunit (TIGR00309; EC 3.6.3.14; HMM-score: 9.9)
  • TheSEED  :
    • Bifunctional autolysin Atl
    • Endo-beta-N-acetylglucosaminidase (EC 3.2.1.96)
    • N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28)
    Cell Wall and Capsule Cell Wall and Capsule - no subcategory Murein Hydrolases  N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28)
    and 4 more
    Cell Wall and Capsule Cell Wall and Capsule - no subcategory Recycling of Peptidoglycan Amino Acids  N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28)
    Regulation and Cell signaling Quorum sensing and biofilm formation Biofilm formation in Staphylococcus  Bifunctional autolysin Atl
    Regulation and Cell signaling Regulation and Cell signaling - no subcategory Cell envelope-associated LytR-CpsA-Psr transcriptional attenuators  Bifunctional autolysin Atl
    Virulence, Disease and Defense Adhesion Adhesins in Staphylococcus  Bifunctional autolysin Atl
  • PFAM:
    Lysozyme (CL0037) Glucosaminidase; Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase (PF01832; HMM-score: 54.1)
    SH3 (CL0010) SH3_8; SH3-like domain (PF13457; HMM-score: 47.2)
    and 3 more
    no clan defined DGOK; 2-keto-3-deoxy-galactonokinase (PF05035; HMM-score: 25.1)
    DUF780; Caenorhabditis elegans protein of unknown function (DUF780) (PF05611; HMM-score: 13.2)
    SH3 (CL0010) SH3_3; Bacterial SH3 domain (PF08239; HMM-score: 10.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 10
    • Internal Helices: 0
  • LocateP: Secretory(released) (with CS)
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: -0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: HQVQAAET
  • SignalP: Signal peptide SP(Sec/SPI) length 29 aa
    • SP(Sec/SPI): 0.997209
    • TAT(Tat/SPI): 0.001289
    • LIPO(Sec/SPII): 0.001006
    • Cleavage Site: CS pos: 29-30. VQA-AE. Pr: 0.9829
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MAKKFNYKLPSMVALTLVGSAVTAHQVQAAETTQDQTTNKNVLDSNKVKATTEQAKAEVKNPTQNISGTQVYQDPAIVQPKTANNKTGNAQVSQKVDTAQVNGDTRANQSATTNNTQPVAKSTSTTAPKTNTNVTNAGYSLVDDEDDNSENQINPELIKSAAKPAALETQYKTAAPKAATTSAPKAKTEATPKVTTFSASAQPRSVAATPKTSLPKYKPQVNSSINDYICKNNLKAPKIEEDYTSYFPKYAYRNGVGRPEGIVVHDTANDRSTINGEISYMKNNYQNAFVHAFVDGDRIIETAPTDYLSWGVGAVGNPRFINVEIVHTHDYASFARSMNNYADYAATQLQYYGLKPDSAEYDGNGTVWTHYAVSKYLGGTDHADPHGYLRSHNYSYDQLYDLINEKYLIKMGKVAPWGTQSTTTPTTPSKPTTPSKPSTGKLTVAANNGVAQIKPTNSGLYTTVYDKTGKATNEVQKTFAVSKTATLGNQKFYLVQDYNSGNKFGWVKEGDVVYNTAKSPVNVNQSYSIKPGTKLYTVPWGTSKQVAGSVSGSGNQTFKASKQQQIDKSIYLYGSVNGKSGWVSKAYLVDTAKPTPTPTPKPSTPTTNNKLTVSSLNGVAQINAKNNGLFTTVYDKTGKPTKEVQKTFAVTKEASLGGNKFYLVKDYNSPTLIGWVKQGDVIYNNAKSPVNVMQTYTVKPGTKLYSVPWGTYKQEAGAVSGTGNQTFKATKQQQIDKSIYLFGTVNGKSGWVSKAYLAVPAAPKKAVAQPKTAVKAYTVTKPQTTQTVSKIAQVKPNNTGIRASVYEKTAKNGAKYADRTFYVTKERAHGNETYVLLNNTSHNIPLGWFNVKDLNVQNLGKEVKTTQKYTVNKSNNGLSMVPWGTKNQVILTGNNIAQGTFNATKQVSVGKDVYLYGTINNRTGWVNAKDLTAPTAVKPTTSAAKDYNYTYVIKNGNGYYYVTPNSDTAKYSLKAFNEQPFAVVKEQVINGQTWYYGKLSNGKLAWIKSTDLAKELIKYNQTGMTLNQVAQIQAGLQYKPQVQRVPGKWTDAKFNDVKHAMDTKRLAQDPALKYQFLRLDQPQNISIDKINQFLKGKGVLENQGAAFNKAAQMYGINEVYLISHALLETGNGTSQLAKGADVVNNKVVTNSNTKYHNVFGIAAYDNDPLREGIKYAKQAGWDTVSKAIVGGAKFIGNSYVKAGQNTLYKMRWNPAHPGTHQYATDVDWANINAKIIKGYYDKIGEVGKYFDIPQYK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: WalR* (activation) regulon, SigB* (activation) regulon
    WalR*(response regulator)important in cell wall homeostasis;  [4]
    SigB*(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation;  [3]   other strains

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 3.2 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)
  4. ↑ Sarah Dubrac, Ivo Gomperts Boneca, Olivier Poupel, Tarek Msadek
    New insights into the WalK/WalR (YycG/YycF) essential signal transduction pathway reveal a major role in controlling cell wall metabolism and biofilm formation in Staphylococcus aureus.
    J Bacteriol: 2007, 189(22);8257-69
    [PubMed:17827301] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

T Oshida, M Sugai, H Komatsuzawa, Y M Hong, H Suginaka, A Tomasz
A Staphylococcus aureus autolysin that has an N-acetylmuramoyl-L-alanine amidase domain and an endo-beta-N-acetylglucosaminidase domain: cloning, sequence analysis, and characterization.
Proc Natl Acad Sci U S A: 1995, 92(1);285-9
[PubMed:7816834] [WorldCat.org] [DOI] (P p)

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