From AureoWiki
Jump to navigation Jump to search

NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01049
  • pan locus tag?: SAUPAN003326000
  • symbol: SAOUHSC_01049
  • pan gene symbol?: potD
  • synonym:
  • product: spermidine/putrescine ABC transporter substrate-binding protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01049
  • symbol: SAOUHSC_01049
  • product: spermidine/putrescine ABC transporter substrate-binding protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1017131..1018204
  • length: 1074
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAAACGATTTTTACAACTCATTATAGGTGCATTAGTTGTGGGTATGCTTTGTCTTACT
    TTAAGTCATTGGTTTAAATCTAAAGAACAAGTGCATACAAATCAAAAAATTTACGTATAC
    AATTGGGGCGAATATATTGATCCAGAGTTAATTAAGAAATTTGAAAAAGAAACTGGCATT
    CAAGTCGTTTATGAAACTTTCGATTCAAATGAAGCGATGGAAGCCAAAATTCGCAATGGC
    GGTACACATTATGATGTTGCTTTTCCTAGTGAATATACAGTTCAAAAATTGAAAAGAGAT
    CATTTATTGTTACCAATAGATCATAATAAGGTACCTAATATTAAAAATTTAGATTCAGAT
    TATATGAATATGTCATTTGATAGAGGCAATAAATATTCTTTACCTTATTTCTTTGGAACT
    GTAGGTATTTTATATAATAAAGAAAAGTATCCAAATGAATCATTTGATAGTTGGAAGTCA
    TTGTATAATCCTAAATTTAAAAACCAAATTTTACTAGTTGACGGTGCTAGAGAGATTATA
    GGCATGAGTTTGAATAAACTTGGGTATAATCTTAATGACCGTAATTCGCACCATTTAAAA
    GAAGCAGAGCGAGATTTAACCAAACTAGCACCACAAGTAAGAGGTGTCGTAGGTGATGAA
    ATTACCATGATGCTTCAACAAAATGAAGGTAACATAGCGGTTGTTTGGAGTGGTGTTGCA
    GCACCTCTAGTGCAAGAAGGGGATAAATATAATTATGTTATTCCTAAAGAAGGATCGAAT
    TTATGGTTCGACAATATGGTAATTCCAAAAACGGCACAAAATAAAGAGGGTGCATATAAA
    TTTATGAATTTTTTATTAGATGCTAAAAATAACAAGCAAAATACAGAATTCGTAGGCTAT
    GCAACGCCAAACAAGGCTGCTCGACAATTGTTGCCTAAAGAGATTAAAGACGACCATCGT
    TTTTATCCGACTAAGAAAGAGCAAGAACGCCTTGAAGTTTATAAAGATTTAGGACCTGAA
    GTTTTAAGTGAATACAATGAAAACTTTTTGAATTTCAAAATGTCATTAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1074

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01049
  • symbol: SAOUHSC_01049
  • description: spermidine/putrescine ABC transporter substrate-binding protein
  • length: 357
  • theoretical pI: 8.29147
  • theoretical MW: 41471.1
  • GRAVY: -0.583754

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Amino acids, peptides and amines putative 2-aminoethylphosphonate ABC transporter, periplasmic 2-aminoethylphosphonate-binding protein (TIGR03261; HMM-score: 23.2)
    and 4 more
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids carbohydrate ABC transporter substrate-binding protein, CPR_0540 family (TIGR03850; HMM-score: 16.1)
    Metabolism Transport and binding proteins Anions molybdate ABC transporter, periplasmic molybdate-binding protein (TIGR01256; HMM-score: 16)
    Metabolism Transport and binding proteins Other ABC transporter periplasmic binding protein, thiB subfamily (TIGR01254; HMM-score: 15.9)
    Metabolism Transport and binding proteins Other thiamin/thiamin pyrophosphate ABC transporter, thiamin/thiamin pyrophospate-binding protein (TIGR01276; HMM-score: 13.8)
  • TheSEED  :
    • ABC transporter, periplasmic spermidine putrescine-binding protein PotD (TC 3.A.1.11.1)
    Amino Acids and Derivatives Arginine; urea cycle, polyamines Polyamine Metabolism  ABC transporter, periplasmic spermidine putrescine-binding protein PotD (TC 3.A.1.11.1)
  • PFAM:
    PBP (CL0177) SBP_bac_8; Bacterial extracellular solute-binding protein (PF13416; HMM-score: 123.8)
    SBP_bac_6; Bacterial extracellular solute-binding protein (PF13343; HMM-score: 102.2)
    and 5 more
    SBP_bac_1; Bacterial extracellular solute-binding protein (PF01547; HMM-score: 74.2)
    SBP_bac_11; Bacterial extracellular solute-binding protein (PF13531; HMM-score: 53.4)
    Lipoprotein_8; Hypothetical lipoprotein (MG045 family) (PF02030; HMM-score: 33.1)
    ABC-2 (CL0181) ABC2_membrane_3; ABC-2 family transporter protein (PF12698; HMM-score: 16.7)
    PBP (CL0177) Phosphonate-bd; ABC transporter, phosphonate, periplasmic substrate-binding protein (PF12974; HMM-score: 13.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helix: 1
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0
    • N-terminally Anchored Score: 2
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: Signal peptide SP(Sec/SPI) length 33 aa
    • SP(Sec/SPI): 0.49937
    • TAT(Tat/SPI): 0.004282
    • LIPO(Sec/SPII): 0.241175
    • Cleavage Site: CS pos: 33-34. VHT-NQ. Pr: 0.1597
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKRFLQLIIGALVVGMLCLTLSHWFKSKEQVHTNQKIYVYNWGEYIDPELIKKFEKETGIQVVYETFDSNEAMEAKIRNGGTHYDVAFPSEYTVQKLKRDHLLLPIDHNKVPNIKNLDSDYMNMSFDRGNKYSLPYFFGTVGILYNKEKYPNESFDSWKSLYNPKFKNQILLVDGAREIIGMSLNKLGYNLNDRNSHHLKEAERDLTKLAPQVRGVVGDEITMMLQQNEGNIAVVWSGVAAPLVQEGDKYNYVIPKEGSNLWFDNMVIPKTAQNKEGAYKFMNFLLDAKNNKQNTEFVGYATPNKAARQLLPKEIKDDHRFYPTKKEQERLEVYKDLGPEVLSEYNENFLNFKMSLK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SAOUHSC_01049&oldid=95113"