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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01271
  • pan locus tag?: SAUPAN003598000
  • symbol: SAOUHSC_01271
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01271
  • symbol: SAOUHSC_01271
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1225887..1226378
  • length: 492
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGAAATCAAGAAAACTGGCTATAACTGCACTTTTAATTGCAATAAATGTTGTATTAAGC
    AGTATTATCATCATTCCTCTAGGACCAGTTAAGGCAGCACCAGTACAGCATTTTGTAAAT
    GTATTAAGTGCGGTCATAGTAGGTCCTTGGTATGGATTAGCTCAAGCGCTTATATCATCA
    ATTTTAAGAGTTCTTTTTGGTACTGGTACAGCTTTTGCATTTCCGGGTAGTATGATTGGA
    GTTTTATTGGCTAGTATGTTTTACATATATCGTAAACATATATTTATGGCCGCGGTCGGT
    GAAGTACTTGGAACTGGTGTCATCGGAAGTTTAATTTGTATACCATTAGCATATTTCCTT
    GGGCTTCAAGACTTCTTCATTAAACCGTTAATGATTACGTTCATAGTCTCAAGTGCTATC
    GGATCTATTATAAGTTATTTCTTATTAATTACTCTAAAAAAACGTGGTATTCTTCAAAGG
    TTTATAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    492

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01271
  • symbol: SAOUHSC_01271
  • description: hypothetical protein
  • length: 163
  • theoretical pI: 10.9181
  • theoretical MW: 17600.5
  • GRAVY: 1.23067

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine energy coupling factor transporter S component ThiW (TIGR02359; HMM-score: 207)
    Metabolism Transport and binding proteins Other energy coupling factor transporter S component ThiW (TIGR02359; HMM-score: 207)
    and 2 more
    Metabolism Transport and binding proteins Unknown substrate ECF transporter S component, folate family (TIGR04518; HMM-score: 36.3)
    Cell structure Cell envelope Surface structures PEP-CTERM protein-sorting domain (TIGR02595; HMM-score: 9)
  • TheSEED  :
    • Substrate-specific component ThiW of predicted thiazole ECF transporter
    Cofactors, Vitamins, Prosthetic Groups, Pigments Cofactors, Vitamins, Prosthetic Groups, Pigments - no subcategory Thiamin biosynthesis  Substrate-specific component ThiW of predicted thiazole ECF transporter
    and 1 more
    Membrane Transport Membrane Transport - no subcategory ECF class transporters  Substrate-specific component ThiW of predicted thiazole ECF transporter
  • PFAM:
    Gx_transp (CL0315) ThiW; Thiamine-precursor transporter protein (ThiW) (PF09512; HMM-score: 181.3)
    and 3 more
    ECF_trnsprt; ECF transporter, substrate-specific component (PF12822; HMM-score: 40.9)
    ECF-ribofla_trS; ECF-type riboflavin transporter, S component (PF07155; HMM-score: 26.6)
    5TM-5TMR_LYT; 5TMR of 5TMR-LYT (PF07694; HMM-score: 10.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: Signal peptide SP(Sec/SPI) length 32 aa
    • SP(Sec/SPI): 0.879749
    • TAT(Tat/SPI): 0.014507
    • LIPO(Sec/SPII): 0.003793
    • Cleavage Site: CS pos: 32-33. VKA-AP. Pr: 0.8741
  • predicted transmembrane helices (TMHMM): 5

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKSRKLAITALLIAINVVLSSIIIIPLGPVKAAPVQHFVNVLSAVIVGPWYGLAQALISSILRVLFGTGTAFAFPGSMIGVLLASMFYIYRKHIFMAAVGEVLGTGVIGSLICIPLAYFLGLQDFFIKPLMITFIVSSAIGSIISYFLLITLKKRGILQRFIK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 3.2 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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