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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01469
  • pan locus tag?: SAUPAN003912000
  • symbol: SAOUHSC_01469
  • pan gene symbol?: nth
  • synonym:
  • product: endonuclease III

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01469
  • symbol: SAOUHSC_01469
  • product: endonuclease III
  • replicon: chromosome
  • strand: -
  • coordinates: 1424647..1425306
  • length: 660
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGTAAGTAAGAAAAAAGCATTAGAAATGATTGATGTTATAGCAAATATGTTTCCGGAT
    GCAGAATGTGAATTAAAACATGATAATCCGTTCGAATTAACTATTGCTGTATTATTGTCA
    GCGCAATGTACAGACGTTCTAGTAAATAGAGTAACAACTGAGCTGTTTAAAAAATACAAA
    ACGCCTGAAGATTATTTGGCAGTCAGTGATGAAGAACTTATGAATGATATTCGTTCTATC
    GGTCTGTATCGTAACAAAGCTAAGAATATTAAGAAACTTTGTCAATCTTTGATTGATCAA
    TTTAATGGAGAAATACCACAAACACATAAGGAATTAGAAAGTTTAGCAGGTGTAGGACGT
    AAAACTGCTAATGTAGTCATGAGTGTAGCATTTGATGAACCTTCATTAGCTGTTGATACG
    CATGTAGAACGTGTTTCTAAACGCTTAGGTATTAATCGTTGGAAAGATAATGTGAGACAG
    GTAGAAGACCGTTTATGTTCTGTCATACCTAGAGATAGATGGAATAGGAGCCATCATCAA
    CTCATTTTCTTTGGAAGATACCACTGTTTAGCCCGAAAACCTAAATGCGATATTTGTCCA
    CTATTAGAAGATTGTAGAGAAGGACAAAAACGTTATAAAGCTAGTTTGAAAGAAGCGTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01469
  • symbol: SAOUHSC_01469
  • description: endonuclease III
  • length: 219
  • theoretical pI: 8.39452
  • theoretical MW: 25234
  • GRAVY: -0.456621

⊟Function[edit | edit source]

  • reaction:
    EC 4.2.99.18?  ExPASy
    DNA-(apurinic or apyrimidinic site) lyase The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair endonuclease III (TIGR01083; EC 4.2.99.18; HMM-score: 273.8)
    and 3 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair A/G-specific adenine glycosylase (TIGR01084; EC 3.2.2.-; HMM-score: 89)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair 8-oxoguanine DNA-glycosylase (ogg) (TIGR00588; EC 3.2.2.-; HMM-score: 21.6)
    Unknown function Enzymes of unknown specificity HAD hydrolase, family IIIA (TIGR01662; HMM-score: 14)
  • TheSEED  :
    • Endonuclease III (EC 4.2.99.18)
    Cell Division and Cell Cycle Cell Division and Cell Cycle - no subcategory Control of cell elongation - division cycle in Bacilli  Endonuclease III (EC 4.2.99.18)
    and 1 more
    DNA Metabolism DNA repair DNA Repair Base Excision  Endonuclease III (EC 4.2.99.18)
  • PFAM:
    HHH (CL0198) HhH-GPD; HhH-GPD superfamily base excision DNA repair protein (PF00730; HMM-score: 82.1)
    and 4 more
    HHH; Helix-hairpin-helix motif (PF00633; HMM-score: 33.7)
    no clan defined EndIII_4Fe-2S; Iron-sulfur binding domain of endonuclease III (PF10576; HMM-score: 23.9)
    DUF2400; Protein of unknown function (DUF2400) (PF09674; HMM-score: 12.6)
    P-loop_NTPase (CL0023) AAA_32; AAA domain (PF13654; HMM-score: 11.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: [4Fe-4S] cluster
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.019353
    • TAT(Tat/SPI): 0.000335
    • LIPO(Sec/SPII): 0.007391
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MVSKKKALEMIDVIANMFPDAECELKHDNPFELTIAVLLSAQCTDVLVNRVTTELFKKYKTPEDYLAVSDEELMNDIRSIGLYRNKAKNIKKLCQSLIDQFNGEIPQTHKELESLAGVGRKTANVVMSVAFDEPSLAVDTHVERVSKRLGINRWKDNVRQVEDRLCSVIPRDRWNRSHHQLIFFGRYHCLARKPKCDICPLLEDCREGQKRYKASLKEA

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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