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NCBI: 03-AUG-2016

Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01471
  • pan locus tag?: SAUPAN003914000
  • symbol: asnC
  • pan gene symbol?: asnC
  • synonym:
  • product: asparaginyl-tRNA synthetase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01471
  • symbol: asnC
  • product: asparaginyl-tRNA synthetase
  • replicon: chromosome
  • strand: -
  • coordinates: 1426310..1427602
  • length: 1293
  • essential: no [1] DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGAAAACAACGATTAAACAAGCGAAAGATCATTTAAACCAAGACGTTACAATTGGTGCT
    TGGTTAACAAATAAACGTTCAAGTGGTAAAATCGCCTTTTTACAATTACGTGATGGAACA
    GGCTTTATGCAAGGCGTAGTAGTTAAATCAGAAGTTGATGAAGAGGTATTCAAACTTGCG
    AAAGAAATTACTCAAGAATCATCTCTATACGTTACAGGCACAATTACAGAAGATAATCGT
    TCTGACTTAGGATACGAAATGCAAGTGAAATCAATTGAAGTTATTTCAGAAGCGCATGAC
    TATCCGATTACACCTAAAAATCATGGTACAGAATTCTTAATGGATCACCGTCATTTATGG
    TTACGTTCTAAAAAACAACATGCTGTAATGAAAATTAGAAATGAAGTTATTCGTGCAACG
    TATGAATTTTTCAACAAAGATGGATTTACAAAGGTTGATCCACCAATTTTGACAGCAAGT
    GCGCCAGAAGGTACAAGTGAATTATTCCATACTAAATACTTTGATCAAGATGCGTTTTTA
    TCTCAAAGTGGTCAGTTATACTTAGAAGCTGCAGCAATGGCACACGGAAAAGTATTTTCA
    TTTGGTCCAACTTTCAGAGCTGAAAAATCAAAAACACGTAGACACTTGATCGAGTTCTGG
    ATGATTGAAGGAGAAATGGCTTTCACAAATCATGCTGAAAGTTTAGAAATTCAAGAACAA
    TATGTAACACATGTTGTAAAATCAGTTTTAGAAAATTGTAAACTAGAGTTGAAAATTTTA
    GAGCGTGATACATCAAAACTTGAAAAAGTTGCGACACCATTCCCTAGAATTTCATATGAT
    GATGCAATTGAATTCTTAAAAGCAGAAGGCTTTGATGATATTGAATGGGGCGAAGATTTT
    GGTGCGCCACATGAAACAGCCATTGCTAATCATTATGATTTACCGGTGTTTATTACTAAT
    TATCCAACTAAAATTAAGCCTTTCTATATGCAACCAAATCCTGAAAATGAAGAAACTGTA
    TTATGTGCAGACTTAATTGCACCTGAAGGATACGGTGAAATTATTGGTGGATCTGAACGT
    GTGGATGACTTAGAATTGTTAGAACAACGCGTTAAAGAACATGGATTAGACGAAGAAGCA
    TATAGTTACTACTTAGACTTACGTCGTTATGGTAGTGTGCCACACTGTGGATTTGGTTTA
    GGTTTAGAGCGTACAGTAGCATGGATTTCTGGTGTTGAACACGTTCGTGAAACAGCGCCA
    TTCCCAAGATTATTAAACCGTTTATATCCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1293

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAOUHSC_01471
  • symbol: AsnC
  • description: asparaginyl-tRNA synthetase
  • length: 430
  • theoretical pI: 5.09478
  • theoretical MW: 49157.2
  • GRAVY: -0.443023

Function[edit | edit source]

  • reaction:
    EC 6.1.1.22?  ExPASy
    Asparagine--tRNA ligase ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn)
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA aminoacylation asparagine--tRNA ligase (TIGR00457; EC 6.1.1.22; HMM-score: 546.9)
    and 6 more
    Genetic information processing Protein synthesis tRNA aminoacylation aspartate--tRNA(Asn) ligase (TIGR00458; EC 6.1.1.23; HMM-score: 336.8)
    Genetic information processing Protein synthesis tRNA aminoacylation aspartate--tRNA ligase (TIGR00459; EC 6.1.1.-; HMM-score: 156.2)
    Genetic information processing Protein synthesis tRNA aminoacylation lysine--tRNA ligase (TIGR00499; EC 6.1.1.6; HMM-score: 97.8)
    Unknown function General EF-P lysine aminoacylase GenX (TIGR00462; HMM-score: 34.4)
    Genetic information processing Protein synthesis tRNA aminoacylation pyrrolysine--tRNA ligase, C-terminal region (TIGR02367; EC 6.1.1.26; HMM-score: 22.1)
    Metabolism Energy metabolism Aerobic pyruvate oxidase (TIGR02720; EC 1.2.3.3; HMM-score: 11.3)
  • TheSEED  :
    • Asparaginyl-tRNA synthetase (EC 6.1.1.22)
    Protein Metabolism Protein biosynthesis tRNA aminoacylation, Glu and Gln  Asparaginyl-tRNA synthetase (EC 6.1.1.22)
    and 1 more
    RNA Metabolism RNA processing and modification tRNA modification Archaea  Asparaginyl-tRNA synthetase (EC 6.1.1.22)
  • PFAM:
    tRNA_synt_II (CL0040) tRNA-synt_2; tRNA synthetases class II (D, K and N) (PF00152; HMM-score: 278.6)
    and 5 more
    OB (CL0021) tRNA_anti-codon; OB-fold nucleic acid binding domain (PF01336; HMM-score: 59.7)
    tRNA_synt_II (CL0040) tRNA-synt_2b; tRNA synthetase class II core domain (G, H, P, S and T) (PF00587; HMM-score: 17.9)
    tRNA-synt_2d; tRNA synthetases class II core domain (F) (PF01409; HMM-score: 15.8)
    no clan defined YliH; Biofilm formation protein (YliH/bssR) (PF10799; HMM-score: 13.1)
    OB (CL0021) tRNA_anti_2; OB-fold nucleic acid binding domain (PF13742; HMM-score: 12.6)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 10
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004447
    • TAT(Tat/SPI): 0.000467
    • LIPO(Sec/SPII): 0.000662
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKTTIKQAKDHLNQDVTIGAWLTNKRSSGKIAFLQLRDGTGFMQGVVVKSEVDEEVFKLAKEITQESSLYVTGTITEDNRSDLGYEMQVKSIEVISEAHDYPITPKNHGTEFLMDHRHLWLRSKKQHAVMKIRNEVIRATYEFFNKDGFTKVDPPILTASAPEGTSELFHTKYFDQDAFLSQSGQLYLEAAAMAHGKVFSFGPTFRAEKSKTRRHLIEFWMIEGEMAFTNHAESLEIQEQYVTHVVKSVLENCKLELKILERDTSKLEKVATPFPRISYDDAIEFLKAEGFDDIEWGEDFGAPHETAIANHYDLPVFITNYPTKIKPFYMQPNPENEETVLCADLIAPEGYGEIIGGSERVDDLELLEQRVKEHGLDEEAYSYYLDLRRYGSVPHCGFGLGLERTVAWISGVEHVRETAPFPRLLNRLYP

Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [2] [3]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SAOUHSC_02503(rpsQ)30S ribosomal protein S17  [4] (data from MRSA252)
    SAOUHSC_01801isocitrate dehydrogenase  [4] (data from MRSA252)
    SAOUHSC_01806pyruvate kinase  [4] (data from MRSA252)
    SAOUHSC_02441alkaline shock protein 23  [4] (data from MRSA252)

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  4. 4.0 4.1 4.2 4.3 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  5. 5.0 5.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit | edit source]