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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01693
  • pan locus tag?: SAUPAN004176000
  • symbol: SAOUHSC_01693
  • pan gene symbol?: comEA
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01693
  • symbol: SAOUHSC_01693
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1604192..1604878
  • length: 687
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAACTCAATGGAAGTTA
    TATATTCTAAGTGCTGTTGTTTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAA
    GATGATTATACTTCAAGAAATTTTGAAAATAAAGATACTGCTCTGAAACAAAGCACTAGT
    GAAAATAGTAGTTTGTCCAAAGTAGAAGATGTCCAGGTCAAAGATGGAGATAATTCCAAA
    AATAAGGGTCCTGTATATGTCGATGTAAAAGGTGCTGTTAAACATCCTAATGTTTATAAA
    ATGACATCTAAGGATAGAGTAGTTGATTTACTTGATAAAGCACAATTATTGGAGGATGCA
    GATGTAAGTCAAATTAATTTGTCTGAAAAATTAACAGATCAAAAAATGATTTTCATACCT
    CATAAAGGACAAAAGAATGTTGAACCACAAATTGAAGTAAACAGTGTGCACGAAAAAAAT
    GGGAACACAAATAATACTAAAGTAAATTTAAATACGGCATCTGTATCAGAATTGATGTCT
    GTTCCTGGAGTTGGGCAAGCTAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGT
    GCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTTTTGGAAGTAAAACTTTTGAT
    AAACTGAAATCTTATTTCACGACATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    687

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01693
  • symbol: SAOUHSC_01693
  • description: hypothetical protein
  • length: 228
  • theoretical pI: 9.36125
  • theoretical MW: 25863.3
  • GRAVY: -0.462719

⊟Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes DNA transformation comEA protein (TIGR01259; HMM-score: 116.3)
    and 4 more
    Cellular processes Cellular processes DNA transformation competence protein ComEA helix-hairpin-helix repeat region (TIGR00426; HMM-score: 74.2)
    Unknown function Enzymes of unknown specificity putative DNA modification/repair radical SAM protein (TIGR03916; HMM-score: 20.7)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair Holliday junction DNA helicase RuvA (TIGR00084; EC 3.6.4.12; HMM-score: 13.3)
    Metabolism Transport and binding proteins Nucleosides, purines and pyrimidines nucleoside Transporter (TIGR00939; HMM-score: 12.4)
  • TheSEED  :
    • Late competence protein ComEA, DNA receptor
    DNA Metabolism DNA uptake, competence Gram Positive Competence  Late competence protein ComEA, DNA receptor
    and 1 more
    DNA Metabolism DNA uptake, competence Late competence  Late competence protein ComEA, DNA receptor
  • PFAM:
    HHH (CL0198) HHH_3; Helix-hairpin-helix motif (PF12836; HMM-score: 89.7)
    and 11 more
    HHH; Helix-hairpin-helix motif (PF00633; HMM-score: 46.1)
    HHH_5; Helix-hairpin-helix domain (PF14520; HMM-score: 29.3)
    Ubiquitin (CL0072) SLBB; SLBB domain (PF10531; HMM-score: 26.9)
    HHH (CL0198) HHH_2; Helix-hairpin-helix motif (PF12826; HMM-score: 22.4)
    HHH_6; Helix-hairpin-helix motif (PF14579; HMM-score: 19.8)
    T2SSK; Type II secretion system (T2SS), protein K (PF03934; HMM-score: 18.3)
    HHH_8; Helix-hairpin-helix domain (PF14716; HMM-score: 16)
    RNA_pol_A_CTD; Bacterial RNA polymerase, alpha chain C terminal domain (PF03118; HMM-score: 14.9)
    PsbU; Photosystem II 12 kDa extrinsic protein (PsbU) (PF06514; HMM-score: 14.8)
    O-anti_assembly (CL0499) Wzy_C; O-Antigen ligase (PF04932; HMM-score: 14.6)
    Aha1_BPI (CL0648) DUF1439; Protein of unknown function (DUF1439) (PF07273; HMM-score: 13.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.17
    • Cytoplasmic Membrane Score: 9.51
    • Cellwall Score: 0.16
    • Extracellular Score: 0.15
    • Internal Helix: 1
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 4
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001648
    • TAT(Tat/SPI): 0.000233
    • LIPO(Sec/SPII): 0.001342
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MVLLYQFLLRYKDFLTQWKLYILSAVVLIMVLIGFIFWRQDDYTSRNFENKDTALKQSTSENSSLSKVEDVQVKDGDNSKNKGPVYVDVKGAVKHPNVYKMTSKDRVVDLLDKAQLLEDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQIEVNSVHEKNGNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLKSYFTT

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • data available for N315

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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