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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1646 [new locus tag: SACOL_RS08395 ]
  • pan locus tag?: SAUPAN004176000
  • symbol: comEA
  • pan gene symbol?: comEA
  • synonym:
  • product: comE operon protein 1-like protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1646 [new locus tag: SACOL_RS08395 ]
  • symbol: comEA
  • product: comE operon protein 1-like protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1678152..1678838
  • length: 687
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAACTCAATGGAAGTTA
    TATATTCTAAGTGCTGTTGTTTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAA
    GATGATTATACTTCAAGAAATTTTGAAAATAAAGATACTGCTCTGAAACAAAGCACTAGT
    GAAAATAGTAGTTTGTCCAAAGTAGAAGATGTCCAGGTCAAAGATGGAGATAATTCCAAA
    AATAAGGGTCCTGTATATGTCGATGTAAAAGGTGCTGTTAAACATCCTAATGTTTATAAA
    ATGACATCTAAGGATAGAGTAGTTGATTTACTTGATAAAGCACAATTATTGGAGGATGCA
    GATGTAAGTCAAATTAATTTGTCTGAAAAATTAACAGATCAAAAAATGATTTTCATACCT
    CATAAAGGACAAAAGAATGTTGAACCACAAATTGAAGTAAACAGTGTGCACGAAAAAAAT
    GGGAACACAAATAATACTAAAGTAAATTTAAATACGGCATCTGTATCAGAATTGATGTCT
    GTTCCTGGAGTTGGGCAAGCTAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGT
    GCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTTTTGGAAGTAAAACTTTTGAT
    AAACTGAAATCTTATTTCACGACATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    687

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL1646 [new locus tag: SACOL_RS08395 ]
  • symbol: ComEA
  • description: comE operon protein 1-like protein
  • length: 228
  • theoretical pI: 9.36125
  • theoretical MW: 25863.3
  • GRAVY: -0.462719

Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes DNA transformation comEA protein (TIGR01259; HMM-score: 116.3)
    and 4 more
    Cellular processes Cellular processes DNA transformation competence protein ComEA helix-hairpin-helix repeat region (TIGR00426; HMM-score: 74.2)
    Unknown function Enzymes of unknown specificity putative DNA modification/repair radical SAM protein (TIGR03916; HMM-score: 20.7)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair Holliday junction DNA helicase RuvA (TIGR00084; EC 3.6.4.12; HMM-score: 13.3)
    Metabolism Transport and binding proteins Nucleosides, purines and pyrimidines nucleoside Transporter (TIGR00939; HMM-score: 12.4)
  • TheSEED  :
    • Late competence protein ComEA, DNA receptor
    DNA Metabolism DNA uptake, competence Gram Positive Competence  Late competence protein ComEA, DNA receptor
    and 1 more
    DNA Metabolism DNA uptake, competence Late competence  Late competence protein ComEA, DNA receptor
  • PFAM:
    HHH (CL0198) HHH_3; Helix-hairpin-helix motif (PF12836; HMM-score: 89.7)
    and 11 more
    HHH; Helix-hairpin-helix motif (PF00633; HMM-score: 46.1)
    HHH_5; Helix-hairpin-helix domain (PF14520; HMM-score: 29.3)
    Ubiquitin (CL0072) SLBB; SLBB domain (PF10531; HMM-score: 26.9)
    HHH (CL0198) HHH_2; Helix-hairpin-helix motif (PF12826; HMM-score: 22.4)
    HHH_6; Helix-hairpin-helix motif (PF14579; HMM-score: 19.8)
    T2SSK; Type II secretion system (T2SS), protein K (PF03934; HMM-score: 18.3)
    HHH_8; Helix-hairpin-helix domain (PF14716; HMM-score: 16)
    RNA_pol_A_CTD; Bacterial RNA polymerase, alpha chain C terminal domain (PF03118; HMM-score: 14.9)
    PsbU; Photosystem II 12 kDa extrinsic protein (PsbU) (PF06514; HMM-score: 14.8)
    O-anti_assembly (CL0499) Wzy_C; O-Antigen ligase (PF04932; HMM-score: 14.6)
    Aha1_BPI (CL0648) DUF1439; Protein of unknown function (DUF1439) (PF07273; HMM-score: 13.7)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.17
    • Cytoplasmic Membrane Score: 9.51
    • Cellwall Score: 0.16
    • Extracellular Score: 0.15
    • Internal Helix: 1
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 4
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001648
    • TAT(Tat/SPI): 0.000233
    • LIPO(Sec/SPII): 0.001342
  • predicted transmembrane helices (TMHMM): 1

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MVLLYQFLLRYKDFLTQWKLYILSAVVLIMVLIGFIFWRQDDYTSRNFENKDTALKQSTSENSSLSKVEDVQVKDGDNSKNKGPVYVDVKGAVKHPNVYKMTSKDRVVDLLDKAQLLEDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQIEVNSVHEKNGNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLKSYFTT

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • data available for N315

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]