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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01846
  • pan locus tag?: SAUPAN004397000
  • symbol: SAOUHSC_01846
  • pan gene symbol?: acsA
  • synonym:
  • product: acetate--CoA ligase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01846
  • symbol: SAOUHSC_01846
  • product: acetate--CoA ligase
  • replicon: chromosome
  • strand: -
  • coordinates: 1752547..1754253
  • length: 1707
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGAAAGTCGAAGTTTATAAAGGAGCGCAAGGTAAACATAACCTTAAAGATTATGAAGAA
    ACATATAATACTTTTGATTGGAAAGACGTAGAACAAGCATTTTCTTGGAGTGAAACTGGA
    AAAATGAACATGGCATATGAATGCATAGATCGCCATGTAGATCAAGGATTAGGGGATAAA
    ATAGCGTTAAATTACAAAGATGAGCACAGAAAAGAATCGTATACTTATAAAGATATGCAA
    CGGTTATCTAATAAAGCAGCGAATGTTTTGTCTGAACATGCAGAAGTTGACAAAGGTGAC
    AGAGTATTTATATTTATGTCGCGTACACCTGAACTATATTTTGCGTTGTTAGGTGTTTTA
    AAAATTGGTGCAATTGTTGGGCCGTTATTTGAAGCATTTATGGAAAAGGCAGTTGCGGAT
    AGATTAGAGAACAGTGAAGCTAAAGTGTTAATTACTAATAAGGCATTGTTACCTCGAGTA
    CCTGTAGATAAATTACCAAACTTGAAAAAAATTGTTGTCGTAGATGAGGATGTAGAAGAC
    AATTACATAGACTTCATTAGTTTGATGGAAACTGCTAGCGATGAATTTGACATTGAATGG
    TTAAAGTCGGATGATGGTTTGATTTTACATTATACATCAGGTTCTACTGGGCAACCTAAA
    GGTGTATTGCATGTTCAACAAGCAATGTTAGTGCACTATATTTCTGGAAAATATGTATTA
    GATTTACAAGAAGATGATGTTTATTGGTGTACAGCAGATCCAGGTTGGGTTACAGGAACA
    TCTTATGGTATTTTTGCACCATGGTTAAATGGCGCTACAAATTGTATAGCTGGTGGTCGC
    TTTTCGCCAGAACAGTGGTATAGTATGATTGAAGATTTTAAAGTGACGATTTGGTATACG
    GCACCAACAGCTTTAAGAATGTTAATGAGTGCTGGTGACGATATTGTTGAGAAATATGAC
    TTGTCATCGTTACGTTCGATTCTATCAGTAGGTGAGCCTTTAAATCCTGAAGTTATAAAA
    TGGGCGAAAAAAGTATACGGTTTAACGGTGTTAGATACTTGGTGGATGACAGAAACAGGT
    GGACATATGATTGTTAACTATCCAACGATGGACGTCAAGCTTGGCTCAATGGGCAAACCA
    TTACCTGGTATTCAAGCTGCAATTATCGATGATGCAGGGAATGAATTACCACCAAATCGA
    ATGGGCAACCTTGCTATAAAAAAAGGCTGGCCATCAATGATGTATCGTATCTGGAAGAAT
    CCAGAAAAATATAAATCATATTTTATTGGAGACTGGTATGTATCTGGTGATTCGGCATAT
    AAAGATGAAGATGGTTACTTCTGGTTCCAAGGACGTGTTGATGATGTAATTATGACAGCT
    GGTGAACGAGTTGGACCATTTGAGGTTGAGTCTAAATTGGTTGAACACGAAGCAGTTGCC
    GAAGCAGGAATTATTGGTAAACCTGATCCGGTTCGCGGTGAAATAATTAAGGCGTTTGTT
    GCACTGAGAAAAGGATATGAACCAACAGACGAATTAAAAGAAGAAATTCGTATATTTGTT
    AAAGAAGGTTTGTCGGCACATGCAGCACCACGTGAAATCGAATTTAAAGATAAATTACCT
    AAAACACGGTCAGGTAAAATTATGAGACGTGTATTAAAAGCTTGGGAATTAAATTTAGAT
    GCTGGGGATTTAAGTACAATGGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1707

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01846
  • symbol: SAOUHSC_01846
  • description: acetate--CoA ligase
  • length: 568
  • theoretical pI: 4.81296
  • theoretical MW: 64364.1
  • GRAVY: -0.33169

⊟Function[edit | edit source]

  • reaction:
    EC 6.2.1.1?  ExPASy
    Acetate--CoA ligase ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA
  • TIGRFAM:
    acetate--CoA ligase (TIGR02188; EC 6.2.1.1; HMM-score: 543.7)
    and 12 more
    propionate--CoA ligase (TIGR02316; EC 6.2.1.17; HMM-score: 306.2)
    benzoate-CoA ligase family (TIGR02262; HMM-score: 287.7)
    cyclohexanecarboxylate-CoA ligase (TIGR03208; EC 6.2.1.-; HMM-score: 199.3)
    dicarboxylate--CoA ligase PimA (TIGR03205; EC 6.2.1.23; HMM-score: 187.8)
    amino acid adenylation domain (TIGR01733; HMM-score: 176.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone O-succinylbenzoate-CoA ligase (TIGR01923; EC 6.2.1.26; HMM-score: 158)
    acyl-CoA ligase (AMP-forming), exosortase A-associated (TIGR03098; HMM-score: 157.9)
    Metabolism Transport and binding proteins Cations and iron carrying compounds (2,3-dihydroxybenzoyl)adenylate synthase (TIGR02275; EC 2.7.7.58; HMM-score: 139.5)
    Metabolism Central intermediary metabolism Other acetoacetate-CoA ligase (TIGR01217; EC 6.2.1.16; HMM-score: 135.2)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan D-alanine--poly(phosphoribitol) ligase, subunit 1 (TIGR01734; EC 6.1.1.13; HMM-score: 97.8)
    4-coumarate--CoA ligase (TIGR02372; EC 6.2.1.12; HMM-score: 53.4)
    L-aminoadipate-semialdehyde dehydrogenase (TIGR03443; EC 1.2.1.31; HMM-score: 36.4)
  • TheSEED  :
    • Acetyl-coenzyme A synthetase (EC 6.2.1.1)
    Carbohydrates Central carbohydrate metabolism Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate  Acetyl-coenzyme A synthetase (EC 6.2.1.1)
  • PFAM:
    ANL (CL0378) AMP-binding; AMP-binding enzyme (PF00501; HMM-score: 317.9)
    and 1 more
    AMP-binding_C (CL0531) AMP-binding_C; AMP-binding enzyme C-terminal domain (PF13193; HMM-score: 82.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 0.83
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.015848
    • TAT(Tat/SPI): 0.000645
    • LIPO(Sec/SPII): 0.00221
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKVEVYKGAQGKHNLKDYEETYNTFDWKDVEQAFSWSETGKMNMAYECIDRHVDQGLGDKIALNYKDEHRKESYTYKDMQRLSNKAANVLSEHAEVDKGDRVFIFMSRTPELYFALLGVLKIGAIVGPLFEAFMEKAVADRLENSEAKVLITNKALLPRVPVDKLPNLKKIVVVDEDVEDNYIDFISLMETASDEFDIEWLKSDDGLILHYTSGSTGQPKGVLHVQQAMLVHYISGKYVLDLQEDDVYWCTADPGWVTGTSYGIFAPWLNGATNCIAGGRFSPEQWYSMIEDFKVTIWYTAPTALRMLMSAGDDIVEKYDLSSLRSILSVGEPLNPEVIKWAKKVYGLTVLDTWWMTETGGHMIVNYPTMDVKLGSMGKPLPGIQAAIIDDAGNELPPNRMGNLAIKKGWPSMMYRIWKNPEKYKSYFIGDWYVSGDSAYKDEDGYFWFQGRVDDVIMTAGERVGPFEVESKLVEHEAVAEAGIIGKPDPVRGEIIKAFVALRKGYEPTDELKEEIRIFVKEGLSAHAAPREIEFKDKLPKTRSGKIMRRVLKAWELNLDAGDLSTME

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: CodY* (repression) regulon, CcpA* regulon
    CodY*(TF)important in Amino acid metabolism; RegPrecise 
    CcpA*(TF)important in Carbon catabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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