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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01886
  • pan locus tag?: SAUPAN004455000
  • symbol: ribH
  • pan gene symbol?: ribH
  • synonym:
  • product: 6,7-dimethyl-8-ribityllumazine synthase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01886
  • symbol: ribH
  • product: 6,7-dimethyl-8-ribityllumazine synthase
  • replicon: chromosome
  • strand: -
  • coordinates: 1798611..1799075
  • length: 465
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGAATTTTGAAGGTAAATTAATTGGAAAAGATTTGAAAGTTGCAATCGTAGTTAGTCGA
    TTTAATGATTTTATCACTGGAAGATTACTTGAAGGTGCAAAAGATACTTTGATTCGACAT
    GATGTTAATGAAGACAATATTGATGTAGCATTTGTGCCAGGTGCGTTTGAAATTCCTTTA
    GTAGCTAAAAAATTAGCCTCATCAGGAAATTATGATGCAGTAATTACATTAGGATGCGTA
    ATTCGCGGTGCTACGTCTCATTATGATTATGTTTGTAATGAAGTCGCGAAAGGTGTTTCT
    AAAGTGAATGATCAAACTAATGTACCAGTCATATTTGGTATTTTAACGACTGAAAGTATT
    GAACAAGCTGTGGAAAGAGCAGGTACGAAAGCTGGCAATAAAGGTGCTGAAGCAGCAGTA
    AGTGCAATTGAAATGGCTAATTTATTAAAATCTATAAAAGCATAG
    60
    120
    180
    240
    300
    360
    420
    465

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01886
  • symbol: RibH
  • description: 6,7-dimethyl-8-ribityllumazine synthase
  • length: 154
  • theoretical pI: 5.78192
  • theoretical MW: 16395.7
  • GRAVY: 0.127273

⊟Function[edit | edit source]

  • reaction:
    EC 2.5.1.9?  ExPASy
    Riboflavin synthase 2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
    EC 2.5.1.78?  ExPASy
    6,7-dimethyl-8-ribityllumazine synthase 1-deoxy-L-glycero-tetrulose 4-phosphate + 5-amino-6-(D-ribitylamino)uracil = 6,7-dimethyl-8-(D-ribityl)lumazine + 2 H2O + phosphate
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Riboflavin, FMN, and FAD 6,7-dimethyl-8-ribityllumazine synthase (TIGR00114; EC 2.5.1.78; HMM-score: 198)
    and 2 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Riboflavin, FMN, and FAD riboflavin synthase (TIGR01506; EC 2.5.1.9; HMM-score: 21.6)
    Metabolism Amino acid biosynthesis Aromatic amino acid family 3-dehydroquinate dehydratase, type II (TIGR01088; EC 4.2.1.10; HMM-score: 12.5)
  • TheSEED  :
    • 6,7-dimethyl-8-ribityllumazine synthase (EC 2.5.1.78)
    Cofactors, Vitamins, Prosthetic Groups, Pigments Riboflavin, FMN, FAD Riboflavin, FMN and FAD metabolism Extended  6,7-dimethyl-8-ribityllumazine synthase (EC 2.5.1.78)
  • PFAM:
    no clan defined DMRL_synthase; 6,7-dimethyl-8-ribityllumazine synthase (PF00885; HMM-score: 202.7)
    and 1 more
    TIM_barrel (CL0036) Trp_syntA; Tryptophan synthase alpha chain (PF00290; HMM-score: 13.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002429
    • TAT(Tat/SPI): 0.000207
    • LIPO(Sec/SPII): 0.000354
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNFEGKLIGKDLKVAIVVSRFNDFITGRLLEGAKDTLIRHDVNEDNIDVAFVPGAFEIPLVAKKLASSGNYDAVITLGCVIRGATSHYDYVCNEVAKGVSKVNDQTNVPVIFGILTTESIEQAVERAGTKAGNKGAEAAVSAIEMANLLKSIKA

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: FMN-box (transcription termination) regulon
    FMN-box(5' cis-acting region)important in Riboflavin biosynthesis; transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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