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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02142
  • pan locus tag?: SAUPAN004956000
  • symbol: SAOUHSC_02142
  • pan gene symbol?: aldH
  • synonym:
  • product: aldehyde dehydrogenase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02142
  • symbol: SAOUHSC_02142
  • product: aldehyde dehydrogenase
  • replicon: chromosome
  • strand: +
  • coordinates: 2014926..2016305
  • length: 1380
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAATATCATTGAGCAAAAATTTTATGACAGTAAAGCTTTTTTCAATACACAACAAACT
    AAAGATATTAGTTTTAGAAAAGAGCAATTAAAGAAGTTAAGCAAAGCTATTAAATCATAC
    GAGAGCGATATTTTAGAAGCACTATATACAGATTTAGGAAAAAATAAAGTCGAAGCTTAT
    GCTACTGAAATTGGCATAACTTTGAAAAGTATCAAAATTGCCCGTAAGGAACTTAAAAAC
    TGGACTAAAACAAAAAATGTAGACACACCTTTATATTTATTTCCAACAAAAAGCTATATC
    AAAAAAGAACCTTATGGAACAGTTTTGATCATTGCACCATTTAACTATCCTTTTCAACTA
    GTGTTCGAACCTTTAATCGGTGCTATTGCAGCAGGTAATACGGCAATTATTAAACCATCT
    GAGTTGACGCCTAATGTTGCACGAGTGATTAAACGGTTAATCAATGAAACATTTGATGCA
    AATTACATTGAAGTTATTGAGGGAGGAATTGAAGAAACGCAAACGTTAATTCACTTACCT
    TTTGACTATGTCTTCTTTACAGGAAGTGAAAATGTAGGCAAAATCGTTTATCAAGCTGCC
    AGCGAAAATTTAGTTCCTGTGACATTAGAAATGGGCGGTAAATCTCCAGTCATCGTTGAT
    GAAACAGCGAATATTAAAGTTGCTAGTGAGCGCATTTGTTTTGGGAAATTCACTAATGCC
    GGCCAAACATGTGTTGCACCAGATTACATTTTAGTACACGAATCTGTAAAAGATGATTTA
    ATCACAGCCCTATCAAAAACGTTGCGTGAATTTTATGGTCAAAATATACAACAAAGTCCA
    GATTATGGCCGCATTGTAAACCTTAAACATTATCATCGTCTGACTTCATTACTTAACAGT
    GCACAAATGAATATTGTATTTGGTGGTCATAGTGATGAGGATGAACGTTATATAGAACCA
    ACATTGTTAGATCACGTTACAAGTGATTCAGCAATTATGCAAGAAGAAATTTTTGGTCCT
    ATCTTACCGATTTTAACGTATCAGTCATTGGATGAAGCAATAGCCTTTATTCACCAAAGA
    CCAAAACCTTTGAGTTTATATTTATTTAGCGAAGATGAAAATGCTACACAACGTGTAATA
    AACGAGCTATCATTTGGCGGCGGCGCTATTAATGATACATTGATGCACCTAGCGAATCCT
    AAATTACCATTTGGTGGTGTTGGTGCCTCAGGTATGGGACGCTATCATGGTAAATATTCA
    TTCGACACTTTTACACATGAAAAAAGCTACATTTTCAAATCTACACGATTAGAATCAGGT
    GTCCATTTACCACCATATAAAGGTAAATTTAAATACATCAAAGCTTTCTTTAAAAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02142
  • symbol: SAOUHSC_02142
  • description: aldehyde dehydrogenase
  • length: 459
  • theoretical pI: 7.12638
  • theoretical MW: 51740.9
  • GRAVY: -0.218736

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Central intermediary metabolism Other succinate-semialdehyde dehydrogenase (TIGR01780; EC 1.2.1.-; HMM-score: 209.5)
    Cellular processes Cellular processes Adaptations to atypical conditions betaine-aldehyde dehydrogenase (TIGR01804; EC 1.2.1.8; HMM-score: 199.4)
    Unknown function Enzymes of unknown specificity aldehyde dehydrogenase, Rv0768 family (TIGR04284; EC 1.2.1.-; HMM-score: 188.6)
    5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase (TIGR02299; EC 1.2.1.60; HMM-score: 182.3)
    Metabolism Energy metabolism Other 2-hydroxymuconic semialdehyde dehydrogenase (TIGR03216; EC 1.2.1.-; HMM-score: 168.3)
    and 11 more
    Metabolism Energy metabolism Amino acids and amines succinylglutamate-semialdehyde dehydrogenase (TIGR03240; EC 1.2.1.71; HMM-score: 157.6)
    putative phosphonoacetaldehyde dehydrogenase (TIGR03250; EC 1.2.1.-; HMM-score: 154.5)
    Metabolism Energy metabolism Amino acids and amines putative delta-1-pyrroline-5-carboxylate dehydrogenase (TIGR01237; EC 1.2.1.88; HMM-score: 146.1)
    1-pyrroline dehydrogenase (TIGR03374; EC 1.2.1.19; HMM-score: 146)
    Metabolism Energy metabolism Amino acids and amines methylmalonate-semialdehyde dehydrogenase (acylating) (TIGR01722; EC 1.2.1.27; HMM-score: 122.2)
    Metabolism Energy metabolism Amino acids and amines delta-1-pyrroline-5-carboxylate dehydrogenase (TIGR01238; EC 1.2.1.88; HMM-score: 99.3)
    Metabolism Energy metabolism Amino acids and amines 1-pyrroline-5-carboxylate dehydrogenase (TIGR01236; EC 1.2.1.88; HMM-score: 91.8)
    phenylacetic acid degradation protein paaN (TIGR02278; HMM-score: 71.3)
    phenylacetic acid degradation protein paaN (TIGR02288; HMM-score: 45.5)
    Metabolism Amino acid biosynthesis Glutamate family glutamate-5-semialdehyde dehydrogenase (TIGR00407; EC 1.2.1.41; HMM-score: 25.1)
    acetaldehyde dehydrogenase (acetylating) (TIGR02518; EC 1.2.1.10; HMM-score: 20.8)
  • TheSEED  :
    • Glyceraldehyde-3-phosphate dehydrogenase (NADP(+)) (EC 1.2.1.9)
    Carbohydrates Central carbohydrate metabolism Methylglyoxal Metabolism  Aldehyde dehydrogenase (EC 1.2.1.3)
    and 2 more
    Carbohydrates Central carbohydrate metabolism Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate  Aldehyde dehydrogenase (EC 1.2.1.3)
    Fatty Acids, Lipids, and Isoprenoids Phospholipids Glycerolipid and Glycerophospholipid Metabolism in Bacteria  Aldehyde dehydrogenase (EC 1.2.1.3)
  • PFAM:
    ALDH-like (CL0099) Aldedh; Aldehyde dehydrogenase family (PF00171; HMM-score: 320.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003667
    • TAT(Tat/SPI): 0.001363
    • LIPO(Sec/SPII): 0.000347
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNIIEQKFYDSKAFFNTQQTKDISFRKEQLKKLSKAIKSYESDILEALYTDLGKNKVEAYATEIGITLKSIKIARKELKNWTKTKNVDTPLYLFPTKSYIKKEPYGTVLIIAPFNYPFQLVFEPLIGAIAAGNTAIIKPSELTPNVARVIKRLINETFDANYIEVIEGGIEETQTLIHLPFDYVFFTGSENVGKIVYQAASENLVPVTLEMGGKSPVIVDETANIKVASERICFGKFTNAGQTCVAPDYILVHESVKDDLITALSKTLREFYGQNIQQSPDYGRIVNLKHYHRLTSLLNSAQMNIVFGGHSDEDERYIEPTLLDHVTSDSAIMQEEIFGPILPILTYQSLDEAIAFIHQRPKPLSLYLFSEDENATQRVINELSFGGGAINDTLMHLANPKLPFGGVGASGMGRYHGKYSFDTFTHEKSYIFKSTRLESGVHLPPYKGKFKYIKAFFKN

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SAOUHSC_01471(asnC)asparaginyl-tRNA synthetase  [3] (data from MRSA252)
    SAOUHSC_00947enoyl-(acyl carrier protein) reductase  [3] (data from MRSA252)
    SAOUHSC_01490DNA-binding protein HU  [3] (data from MRSA252)
    SAOUHSC_01801isocitrate dehydrogenase  [3] (data from MRSA252)
    SAOUHSC_01814hypothetical protein  [3] (data from MRSA252)
    SAOUHSC_02485DNA-directed RNA polymerase subunit alpha  [3] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 3.2 3.3 3.4 3.5 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  4. ↑ 4.0 4.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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