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NCBI: 03-AUG-2016
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus NCTC8325
- locus tag: SAOUHSC_02576
- pan locus tag?: SAUPAN005772000
- symbol: SAOUHSC_02576
- pan gene symbol?: —
- synonym:
- product: secretory antigen SsaA
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAOUHSC_02576
- symbol: SAOUHSC_02576
- product: secretory antigen SsaA
- replicon: chromosome
- strand: +
- coordinates: 2366718..2367218
- length: 501
- essential: no DEG other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3921573 NCBI
- RefSeq: YP_501037 NCBI
- BioCyc: G1I0R-2432 BioCyc
- MicrobesOnline: 1291008 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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481ATGAAAAAATTAGTAACAGCAACTACGTTAACAGCAGGAATCGGCACAGCATTAGTAGGT
CAAGCACATCATGCAGATGCTGCTGAAAATTATACAAATTACAACAACTATAACTACAAC
ACGACTCAAACTACAACGACTACGACAACTACGACAACTACATCATCAATTTCACATTCT
GGTAACTTATACACTGCAGGACAATGTACTTGGTATGTATATGATAAAGTTGGCGGAGAA
ATCGGTTCTACTTGGGGAAATGCTAATAATTGGGCTGCTGCTGCACAAGGTGCTGGATTC
ACAGTAAATCATACACCTTCTAAAGGCGCTATCCTACAATCTTCTGAAGGACCATTTGGT
CACGTTGCATATGTAGAAAGTGTAAACAGTGATGGTTCAGTTACAATTTCAGAAATGAAT
TATAGTGGCGGACCTTTCTCAGTAAGTTCTAGAACTATTTCTGCAAGTGAAGCAGGTAAC
TACAACTACATCCATATTTAA60
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501
⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAOUHSC_02576
- symbol: SAOUHSC_02576
- description: secretory antigen SsaA
- length: 166
- theoretical pI: 6.21564
- theoretical MW: 17398.7
- GRAVY: -0.351205
⊟Function[edit | edit source]
- TIGRFAM: Cell envelope Surface structures pilus (MSHA type) biogenesis protein MshL (TIGR02519; HMM-score: 5.4)
- TheSEED :
- Secretory antigen SsaA-like protein SAV2304
- PFAM: Peptidase_CA (CL0125) CHAP; CHAP domain (PF05257; HMM-score: 53.4)and 1 moreno clan defined Dicty_REP; Dictyostelium (Slime Mold) REP protein (PF05086; HMM-score: 4.8)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Extracellular
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0.02
- Extracellular Score: 9.98
- Internal Helices: 0
- LocateP: Secretory(released) (with CS)
- Prediction by SwissProt Classification: Extracellular
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: -0.17
- Signal peptide possibility: 1
- N-terminally Anchored Score: -2
- Predicted Cleavage Site: HHADAAEN
- SignalP: Signal peptide SP(Sec/SPI) length 27 aa
- SP(Sec/SPI): 0.996663
- TAT(Tat/SPI): 0.001506
- LIPO(Sec/SPII): 0.001376
- Cleavage Site: CS pos: 27-28. ADA-AE. Pr: 0.9730
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MKKLVTATTLTAGIGTALVGQAHHADAAENYTNYNNYNYNTTQTTTTTTTTTTTSSISHSGNLYTAGQCTWYVYDKVGGEIGSTWGNANNWAAAAQGAGFTVNHTPSKGAILQSSEGPFGHVAYVESVNSDGSVTISEMNYSGGPFSVSSRTISASEAGNYNYIHI
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas [1] [2]
- protein localization: data available for COL
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- predicted SigA promoter [3] : S993 > SAOUHSC_02576
⊟Regulation[edit | edit source]
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: [3]
Multi-gene expression profiles
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
Proteomics: 2015, 15(21);3648-61
[PubMed:26224020] [WorldCat.org] [DOI] (I p) - ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
Sci Rep: 2017, 7(1);9718
[PubMed:28887440] [WorldCat.org] [DOI] (I e) - ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
PLoS Genet: 2016, 12(4);e1005962
[PubMed:27035918] [WorldCat.org] [DOI] (I e) - ↑ Sarah Dubrac, Ivo Gomperts Boneca, Olivier Poupel, Tarek Msadek
New insights into the WalK/WalR (YycG/YycF) essential signal transduction pathway reveal a major role in controlling cell wall metabolism and biofilm formation in Staphylococcus aureus.
J Bacteriol: 2007, 189(22);8257-69
[PubMed:17827301] [WorldCat.org] [DOI] (I p)