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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02595
  • pan locus tag?: SAUPAN005801000
  • symbol: SAOUHSC_02595
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02595
  • symbol: SAOUHSC_02595
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2384973..2385890
  • length: 918
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGTTTCAAAAATTGAGTATATTTGCCACGAAGAGTTTTTTGGTATGGATGTTAGTAGCA
    GCTGTTATTGGATTTATTTTCCCACAACATGTTGCGACATTAGGTAAATGGGTACCTTAT
    TTACTTGGTATTGTTATGTTAGGTATGGGATTAACAATTACACCTAATGATTTCAAAATG
    GTCTTTAAAGCACCTAGAGCAGTAATTATTGGTGTCTGTCTACAATTCAGTATTATGCCC
    ACATTAGCATTTATAATTGCAAAGTCTTTTCATTTACCACCTGATATTGCTGTTGGCGTA
    ATATTAGTTGGATGTTGTCCGGGTGGGACATCAAGTAATGTAATGAGTTATTTAGCCAAA
    GCTAACGTAGCACTTTCTGTTTCTATTACGACGGTCTCTACGTTGCTAGCGCCATTCGTT
    ACACCTGCGTTAATATATCTATTTGCAAATGAATGGTTGGAAGTATCTTTCGTGAGTATG
    TTGTGGTCAGTTGTTCAAGTTGTATTAATTCCAATTGCTTTAGGTATTGTTTTGCAAATT
    ATTAATCGTAAAATTGCTGAAAAAGCTTCTACAGCTTTGCCAATTATATCAGTTGTTGCT
    ATTTCATTAATTTTAGCAATAGTTGTAGGTGGCAGTAAGCACCAAATCTTAACTACAGGA
    TTATTAATATTTTTAGTAGTTATTTTACATAACGTATTAGGGTATACGATTGGATATTGG
    TTAGCTCGTCTTTTAAAATTAGATCGACAAGATCAAAAAGCAGTCAGTATTGAAGTTGGA
    ATGCAGAACTCTGGTTTAGCTGTGTCATTAGCAGCATTGCATTTTAATCCAATTGCAGCA
    GTACCAGGCGCAGTGTTTAGTTTCATTCATAATATAACAGGGCCTATTTTAGCAAAGTAT
    TGGTCAAAAAAGTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    918

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02595
  • symbol: SAOUHSC_02595
  • description: hypothetical protein
  • length: 305
  • theoretical pI: 10.2753
  • theoretical MW: 32851.6
  • GRAVY: 1.06951

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids bile acid transporter (TIGR00841; HMM-score: 289)
    and 1 more
    membrane protein AbrB duplication (TIGR03082; HMM-score: 11.3)
  • TheSEED  :
    • COG0385 sodium-dependent transporter
    dcernst CoA Salvage2  COG0385 sodium-dependent transporter
  • PFAM:
    CPA_AT (CL0064) SBF; Sodium Bile acid symporter family (PF01758; HMM-score: 155.2)
    and 3 more
    SBF_like; SBF-like CPA transporter family (DUF4137) (PF13593; HMM-score: 77.2)
    Mem_trans; Membrane transport protein (PF03547; HMM-score: 10.4)
    no clan defined DUF3464; Protein of unknown function (DUF3464) (PF11947; HMM-score: 6.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 8
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.025516
    • TAT(Tat/SPI): 0.001144
    • LIPO(Sec/SPII): 0.015437
  • predicted transmembrane helices (TMHMM): 9

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MFQKLSIFATKSFLVWMLVAAVIGFIFPQHVATLGKWVPYLLGIVMLGMGLTITPNDFKMVFKAPRAVIIGVCLQFSIMPTLAFIIAKSFHLPPDIAVGVILVGCCPGGTSSNVMSYLAKANVALSVSITTVSTLLAPFVTPALIYLFANEWLEVSFVSMLWSVVQVVLIPIALGIVLQIINRKIAEKASTALPIISVVAISLILAIVVGGSKHQILTTGLLIFLVVILHNVLGYTIGYWLARLLKLDRQDQKAVSIEVGMQNSGLAVSLAALHFNPIAAVPGAVFSFIHNITGPILAKYWSKKL

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY* (repression) regulon
    CodY*(TF)important in Amino acid metabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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