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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02652
  • pan locus tag?: SAUPAN005886000
  • symbol: SAOUHSC_02652
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02652
  • symbol: SAOUHSC_02652
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 2438502..2439404
  • length: 903
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    GTGCTGATTAAAGTACATTACTCTGGTATAAATTATAAAGATGCTTTAGCGACTCAAGAT
    CATAATGCAGTCGTAAAATCGTATCCTATGATTCCAGGAATAGATTTAGCTGGAACAATT
    GTTGAATCCGAAGCACCAGGCTTTGAAAAAGGAGAACAAGTAATTGTAACGAGTTATGAC
    CTAGGTGTCAGCCATTATGGCGGTTTTAGTGAATATGCGCGTGTAAAATCAGAATGGATT
    ATCAAGCTTCCTGATACTTTAACATTAGAAGAATCAATGATATATGGCACAGCTGGTTAT
    ACTGCCGGTTTAGCAATTGAAAGACTTGAAAAAGTTGGAATGAATATTGAAGATGGTCCT
    GTACTCGTTCGCGGTGCTTCAGGTGGTGTCGGTACTTTAGCAGTACTCATGCTTAATGAA
    CTTGGTTATAAAGTTATCGCAAGTACAGGTAAACAAGATGTTAGCGATCAATTACTTGAA
    CTTGGTGCCAAAGAAGTTATCGATCGACTTCCTGTTGAAGATGATCATAAAAAGCCACTC
    GCATCATCAACTTGGCAAGCTTGTGTAGACCCTGTTGGTGGCGAAGGTATTAATTATGTT
    ACAAAGCGTTTAAATCATAGTGGGTCAATTACAGTTATTGGTATGACTGCCGGTAATACT
    TATACTAATTCTGTATTCCCTCACATTTTAAGAGGTGTAAACATTTTAGGAATTGACTCG
    GTATTTACTGCTATGAAATTAAGACAGCGCGTTTGGCGTCGTCTCGCAAAAGATTTAATG
    CCTGAAAATTTACATGAGATCAAGCAAGTTATTACATTTGATGAACTTCCAGAACAACTT
    AACAAAGTAATTAAACATGAAAATAAAGGGCGCATTGTTATCGATTTCGGTGTAGATAAA
    TAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    903

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02652
  • symbol: SAOUHSC_02652
  • description: hypothetical protein
  • length: 300
  • theoretical pI: 5.87292
  • theoretical MW: 32865.5
  • GRAVY: -0.0836667

⊟Function[edit | edit source]

  • TIGRFAM:
    Unknown function Enzymes of unknown specificity putative quinone oxidoreductase, YhdH/YhfP family (TIGR02823; HMM-score: 421.9)
    and 7 more
    Unknown function Enzymes of unknown specificity putative NAD(P)H quinone oxidoreductase, PIG3 family (TIGR02824; HMM-score: 89.7)
    crotonyl-CoA carboxylase/reductase (TIGR01751; EC 1.3.1.85; HMM-score: 51.5)
    Metabolism Energy metabolism Fermentation zinc-binding alcohol dehydrogenase family protein (TIGR02817; HMM-score: 35.9)
    leukotriene B4 12-hydroxydehydrogenase/15-oxo-prostaglandin 13-reductase (TIGR02825; EC 1.3.1.48,1.3.1.74; HMM-score: 33.8)
    Unknown function Enzymes of unknown specificity NDMA-dependent alcohol dehydrogenase, Rxyl_3153 family (TIGR03989; EC 1.1.99.36; HMM-score: 25.7)
    Metabolism Energy metabolism Amino acids and amines L-threonine 3-dehydrogenase (TIGR00692; EC 1.1.1.103; HMM-score: 24.4)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll chlorophyll synthesis pathway protein BchC (TIGR01202; HMM-score: 18.2)
  • TheSEED  :
    • Acryloyl-CoA reductase AcuI/YhdH (EC 1.3.1.84)
    Carbohydrates Fermentation Fermentations: Lactate  Alcohol dehydrogenase (EC 1.1.1.1)
    and 2 more
    Carbohydrates Fermentation Fermentations: Mixed acid  Alcohol dehydrogenase (EC 1.1.1.1)
    Fatty Acids, Lipids, and Isoprenoids Phospholipids Glycerolipid and Glycerophospholipid Metabolism in Bacteria  Alcohol dehydrogenase (EC 1.1.1.1)
  • PFAM:
    NADP_Rossmann (CL0063) ADH_zinc_N; Zinc-binding dehydrogenase (PF00107; HMM-score: 46.8)
    and 4 more
    GroES (CL0296) ADH_N; Alcohol dehydrogenase GroES-like domain (PF08240; HMM-score: 30.1)
    NADP_Rossmann (CL0063) ADH_zinc_N_2; Zinc-binding dehydrogenase (PF13602; HMM-score: 15.3)
    ELFV_dehydrog; Glutamate/Leucine/Phenylalanine/Valine dehydrogenase (PF00208; HMM-score: 12.2)
    Methyltransf_32; Methyltransferase domain (PF13679; HMM-score: 11.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.012456
    • TAT(Tat/SPI): 0.000311
    • LIPO(Sec/SPII): 0.001529
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MLIKVHYSGINYKDALATQDHNAVVKSYPMIPGIDLAGTIVESEAPGFEKGEQVIVTSYDLGVSHYGGFSEYARVKSEWIIKLPDTLTLEESMIYGTAGYTAGLAIERLEKVGMNIEDGPVLVRGASGGVGTLAVLMLNELGYKVIASTGKQDVSDQLLELGAKEVIDRLPVEDDHKKPLASSTWQACVDPVGGEGINYVTKRLNHSGSITVIGMTAGNTYTNSVFPHILRGVNILGIDSVFTAMKLRQRVWRRLAKDLMPENLHEIKQVITFDELPEQLNKVIKHENKGRIVIDFGVDK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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