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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2317 [new locus tag: SAUSA300_RS12800 ]
  • pan locus tag?: SAUPAN005886000
  • symbol: SAUSA300_2317
  • pan gene symbol?:
  • synonym:
  • product: putative zinc-binding dehydrogenase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2317 [new locus tag: SAUSA300_RS12800 ]
  • symbol: SAUSA300_2317
  • product: putative zinc-binding dehydrogenase
  • replicon: chromosome
  • strand: +
  • coordinates: 2491405..2492307
  • length: 903
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    GTGCTGATTAAAGTACATTACTCTGGTATAAATTATAAAGATGCTTTAGCGACTCAAGAT
    CATAATGCAGTCGTAAAATCGTATCCTATGATTCCAGGAATAGATTTAGCTGGAACAATT
    GTTGAATCCGAAGCACCAGGCTTTGAAAAAGGAGAACAAGTAATTGTAACGAGTTATGAC
    CTAGGTGTCAGCCATTATGGCGGTTTTAGTGAATATGCGCGTGTAAAATCAGAATGGATT
    ATCAAGCTTCCTGATACTTTAACATTAGAAGAATCAATGATATATGGCACAGCTGGTTAT
    ACTGCCGGTTTAGCAATTGAAAGACTTGAAAAAGTTGGAATGAATATTGAAGATGGTCCT
    GTACTCGTTCGCGGTGCTTCAGGTGGTGTCGGTACTTTAGCAGTACTCATGCTTAATGAA
    CTTGGTTATAAAGTTATCGCAAGTACAGGTAAACAAGATGTTAGCGATCAATTACTTGAA
    CTTGGTGCCAAAGAAGTTATCGATCGACTTCCTGTTGAAGATGATCATAAAAAGCCACTC
    GCATCATCAACTTGGCAAGCTTGTGTAGACCCTGTTGGTGGCGAAGGTATTAATTATGTT
    ACAAAGCGTTTAAATCATAGTGGGTCAATTGCAGTTATTGGTATGACTGCCGGTAATACT
    TATACTAATTCTGTATTCCCTCACATTTTAAGAGGTGTAAACATTTTAGGAATTGACTCG
    GTATTTACTGCTATGAAATTAAGACAGCGCGTTTGGCGTCGTCTCGCAAAAGATTTAATG
    CCTGAAAATTTACATGAGATCAAGCAAGTTATTACATTTGATGAACTTCCAGAACAACTT
    AACAAAGTAATTAAACATGAAAATAAAGGGCGCATTGTTATCGATTTCGGTGTAGATAAA
    TAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    903

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_2317 [new locus tag: SAUSA300_RS12800 ]
  • symbol: SAUSA300_2317
  • description: putative zinc-binding dehydrogenase
  • length: 300
  • theoretical pI: 5.87292
  • theoretical MW: 32835.5
  • GRAVY: -0.0753333

Function[edit | edit source]

  • TIGRFAM:
    Unknown function Enzymes of unknown specificity putative quinone oxidoreductase, YhdH/YhfP family (TIGR02823; HMM-score: 422.5)
    and 7 more
    Unknown function Enzymes of unknown specificity putative NAD(P)H quinone oxidoreductase, PIG3 family (TIGR02824; HMM-score: 89.7)
    crotonyl-CoA carboxylase/reductase (TIGR01751; EC 1.3.1.85; HMM-score: 51.3)
    Metabolism Energy metabolism Fermentation zinc-binding alcohol dehydrogenase family protein (TIGR02817; HMM-score: 35.9)
    leukotriene B4 12-hydroxydehydrogenase/15-oxo-prostaglandin 13-reductase (TIGR02825; EC 1.3.1.48,1.3.1.74; HMM-score: 35.7)
    Unknown function Enzymes of unknown specificity NDMA-dependent alcohol dehydrogenase, Rxyl_3153 family (TIGR03989; EC 1.1.99.36; HMM-score: 25.5)
    Metabolism Energy metabolism Amino acids and amines L-threonine 3-dehydrogenase (TIGR00692; EC 1.1.1.103; HMM-score: 25)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll chlorophyll synthesis pathway protein BchC (TIGR01202; HMM-score: 18.1)
  • TheSEED:  
    Carbohydrates Fermentation Fermentations: Lactate  Alcohol dehydrogenase (EC 1.1.1.1)
    and 2 more
    Fermentations: Mixed acid  Alcohol dehydrogenase (EC 1.1.1.1)
    Fatty Acids, Lipids, and Isoprenoids Phospholipids Glycerolipid and Glycerophospholipid Metabolism in Bacteria  Alcohol dehydrogenase (EC 1.1.1.1)
  • PFAM:
    NADP_Rossmann (CL0063) ADH_zinc_N; Zinc-binding dehydrogenase (PF00107; HMM-score: 47.1)
    and 3 more
    GroES (CL0296) ADH_N; Alcohol dehydrogenase GroES-like domain (PF08240; HMM-score: 30.1)
    NADP_Rossmann (CL0063) ADH_zinc_N_2; Zinc-binding dehydrogenase (PF13602; HMM-score: 15.7)
    ELFV_dehydrog; Glutamate/Leucine/Phenylalanine/Valine dehydrogenase (PF00208; HMM-score: 12.1)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.012456
    • TAT(Tat/SPI): 0.000311
    • LIPO(Sec/SPII): 0.001529
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MLIKVHYSGINYKDALATQDHNAVVKSYPMIPGIDLAGTIVESEAPGFEKGEQVIVTSYDLGVSHYGGFSEYARVKSEWIIKLPDTLTLEESMIYGTAGYTAGLAIERLEKVGMNIEDGPVLVRGASGGVGTLAVLMLNELGYKVIASTGKQDVSDQLLELGAKEVIDRLPVEDDHKKPLASSTWQACVDPVGGEGINYVTKRLNHSGSIAVIGMTAGNTYTNSVFPHILRGVNILGIDSVFTAMKLRQRVWRRLAKDLMPENLHEIKQVITFDELPEQLNKVIKHENKGRIVIDFGVDK

Experimental data[edit | edit source]

  • experimentally validated: no data available

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

  • SAUSA300_2317 no polycistronic organisation predicted

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]