From AureoWiki
Jump to navigation Jump to search

NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02937
  • pan locus tag?: SAUPAN006318000
  • symbol: SAOUHSC_02937
  • pan gene symbol?: cudT
  • synonym:
  • product: choline transporter

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02937
  • symbol: SAOUHSC_02937
  • product: choline transporter
  • replicon: chromosome
  • strand: -
  • coordinates: 2703339..2704961
  • length: 1623
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    TTGAAAAAAGAGAAAGTTATGGACTGGACGACCTTTATAGGGACAGTAGCTGTACTTCTT
    TTTGCAGTTATACCTATGATGGCTTTTCCAAAAGCAAGTGAAGATATCATCACTGGTATT
    AATAGTGCCATTTCTGATTCAATTGGTTCGATATATTTATTTATGGGGCTGGCGATTTTT
    TGCTTTGTAATGTATATCGCATTTGGTAAATATGGTAATGTCACACTTGGTAAAGCAAGT
    GATAAACCAGAATTTAATACATTTACATGGGCGGCAATGCTGTTTTGTGCAGGCATAGGC
    TCTGATATTTTATACTGGGGCGTTATTGAATGGGCTTTTTACTATCAAGTTCCACCAAAT
    GGCGCGAAAAGTATGAGTGATGAAGCACTCCAATATGCGACGCAATATGGTATGTTCCAC
    TGGGGGCCAATTGCTTGGGCTATTTATGTTCTACCAGCATTACCAATTGGTTATTTAGTA
    TTTGTTAAAAAACAACCGGTGTATAAAATTAGTCAAGCTTGTCGTCCGATTTTAAAAGGT
    CAAACAGATAAATTTGTAGGTAAAGTTGTAGATATCTTATTTATCTTTGGATTGCTAGGT
    GGTGCGGCAACATCACTAGCGTTAGGTGTGCCATTAATTTCTGCAGGCATAGAAAGATTA
    ACTGGTTTAGATGGTAAAAATATGATTTTACGTTCGGCCATTTTATTAACAATCACGGTT
    ATATTTGCCATTAGTTCATATACAGGATTGAAAAAAGGTATTCAAAAGTTAAGTGATATC
    AACGTTTGGCTATCCTTTGTACTTTTAGCCTTTATATTTATTATTGGACCGACTGTTTTT
    ATTATGGAAACGACAGTGACAGGGTTCGGAAATATGTTGAGAGATTTCTTTCATATGGCA
    ACATGGTTAGAACCATTCGGTGGTATTAAAGGTCGAAAAGAAACGAATTTCCCACAAGAC
    TGGACAATATTCTACTGGTCATGGTGGTTAGTATATGCGCCATTTATCGGTTTATTTATC
    GCTAGAATTTCAAAAGGTCGACGCCTTAAAGAAGTCGTGCTAGGAACAATTATTTATGGA
    ACGCTTGGATGCGTATTATTCTTTGGTATTTTTGGTAACTATGCTGTGTATTTACAAATT
    TCTGGACAGTTTAATGTAACACAATATTTAAATACACATGGTACAGAGGCAACCATTATT
    GAAGTGGTGCATCATTTACCATTCCCATCATTGATGATTGTACTATTCTTAGTATCTGCT
    TTCTTATTCTTAGCAACAACATTTGATTCGGGTTCATATATTTTAGCGGCAGCATCTCAG
    AAAAAAGTGGTAGGCGAACCATTACGTGCCAATCGTTTATTCTGGGCATTTGCATTGTGC
    TTATTGCCATTTTCATTGATGCTAGTTGGTGGTGAACGTGCATTAGAAGTATTGAAAACT
    GCTTCAATACTGGCAAGTGTGCCATTAATTGTTATTTTTATTTTCATGATGATATCATTT
    TTAATCATTTTAGGGCGCGATAGAATTAAACTTGAAACGCGTGCTGAAAAATTAAAAGAA
    GTTGAACGTCGTTCATTGCGAATCGTTCAAGTATCAGAAGAAGAACAAGACGATAATTTA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1623

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02937
  • symbol: SAOUHSC_02937
  • description: choline transporter
  • length: 540
  • theoretical pI: 9.09649
  • theoretical MW: 60254
  • GRAVY: 0.619259

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Other transporter, betaine/carnitine/choline transporter (BCCT) family (TIGR00842; HMM-score: 460.9)
  • TheSEED  :
    • Betaine/carnitine/choline transporter (BCCT) family
    Stress Response Osmotic stress Choline and Betaine Uptake and Betaine Biosynthesis  High-affinity choline uptake protein BetT
  • PFAM:
    APC (CL0062) BCCT; BCCT, betaine/carnitine/choline family transporter (PF02028; HMM-score: 502.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.242236
    • TAT(Tat/SPI): 0.005112
    • LIPO(Sec/SPII): 0.056956
  • predicted transmembrane helices (TMHMM): 12

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKKEKVMDWTTFIGTVAVLLFAVIPMMAFPKASEDIITGINSAISDSIGSIYLFMGLAIFCFVMYIAFGKYGNVTLGKASDKPEFNTFTWAAMLFCAGIGSDILYWGVIEWAFYYQVPPNGAKSMSDEALQYATQYGMFHWGPIAWAIYVLPALPIGYLVFVKKQPVYKISQACRPILKGQTDKFVGKVVDILFIFGLLGGAATSLALGVPLISAGIERLTGLDGKNMILRSAILLTITVIFAISSYTGLKKGIQKLSDINVWLSFVLLAFIFIIGPTVFIMETTVTGFGNMLRDFFHMATWLEPFGGIKGRKETNFPQDWTIFYWSWWLVYAPFIGLFIARISKGRRLKEVVLGTIIYGTLGCVLFFGIFGNYAVYLQISGQFNVTQYLNTHGTEATIIEVVHHLPFPSLMIVLFLVSAFLFLATTFDSGSYILAAASQKKVVGEPLRANRLFWAFALCLLPFSLMLVGGERALEVLKTASILASVPLIVIFIFMMISFLIILGRDRIKLETRAEKLKEVERRSLRIVQVSEEEQDDNL

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • data available for N315

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SAOUHSC_02937&oldid=86021"