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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_2549 [new locus tag: SAUSA300_RS14155 ]
- pan locus tag?: SAUPAN006318000
- symbol: bccT
- pan gene symbol?: cudT
- synonym:
- product: BCCT family choline/carnitine/betaine transporter
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_2549 [new locus tag: SAUSA300_RS14155 ]
- symbol: bccT
- product: BCCT family choline/carnitine/betaine transporter
- replicon: chromosome
- strand: -
- coordinates: 2754711..2756333
- length: 1623
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3915059 NCBI
- RefSeq: YP_495183 NCBI
- BioCyc: see SAUSA300_RS14155
- MicrobesOnline: 1294064 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1621TTGAAAAAAGAGAAAGTTATGGACTGGACGACCTTTATAGGGACAGTAGCTGTACTTCTT
TTTGCAGTTATACCTATGATGGCTTTTCCAAAAGCAAGTGAAGATATCATCACTGGTATT
AATAGTGCCATTTCTGATTCAATTGGTTCGATATATTTATTTATGGGGCTGGCGATTTTT
TGCTTTGTAATGTATATCGCATTTGGTAAATATGGTAATGTCACACTTGGTAAAGCAAGT
GATAAACCAGAATTTAATACATTTACATGGGCGGCAATGCTGTTTTGTGCAGGCATAGGC
TCTGATATTTTATACTGGGGCGTTATTGAATGGGCTTTTTACTATCAAGTTCCACCAAAT
GGCGCGAAAAGTATGAGTGATGAAGCACTCCAATATGCGACGCAATATGGTATGTTCCAC
TGGGGGCCAATTGCTTGGGCTATTTATGTTCTACCAGCATTACCAATTGGTTATTTAGTA
TTTGTTAAAAAACAACCGGTGTATAAAATTAGTCAAGCTTGTCGTCCGATTTTAAAAGGT
CAAACAGATAAATTTGTAGGTAAAGTTGTAGATATCTTATTTATCTTTGGATTGCTAGGT
GGTGCGGCAACATCACTAGCGTTAGGTGTGCCATTAATTTCTGCAGGCATAGAAAGATTA
ACTGGTTTAGATGGTAAAAATATGATTTTACGTTCGGCCATTTTATTAACAATCACGGTT
ATATTTGCCATTAGTTCATATACAGGATTGAAAAAAGGTATTCAAAAGTTAAGTGATATC
AACGTTTGGCTATCCTTTGTACTTTTAGCCTTTATATTTATTATTGGACCGACTGTTTTT
ATTATGGAAACGACAGTGACAGGGTTCGGAAATATGTTGAGAGATTTCTTTCATATGGCA
ACATGGTTAGAACCATTCGGTGGTATTAAAGGTCGAAAAGAAACGAATTTCCCACAAGAC
TGGACAATATTCTACTGGTCATGGTGGTTAGTATATGCGCCATTTATCGGTTTATTTATC
GCTAGAATTTCAAAAGGTCGACGCCTTAAAGAAGTCGTGCTAGGAACAATTATTTATGGA
ACGCTTGGATGCGTATTATTCTTTGGTATTTTTGGTAACTATGCTGTGTATTTACAAATT
TCTGGACAGTTTAATGTAACACAATATTTAAATACACATGGTACAGAGGCAACCATTATT
GAAGTGGTGCATCATTTACCATTCCCATCATTGATGATTGTACTATTCTTAGTATCTGCT
TTCTTATTCTTAGCAACAACATTTGATTCGGGTTCATATATTTTAGCGGCAGCATCTCAG
AAAAAAGTGGTAGGCGAACCATTACGTGCCAATCGTTTATTCTGGGCATTTGCATTGTGC
TTATTGCCATTTTCATTGATGCTAGTTGGTGGTGAACGTGCATTAGAAGTATTGAAAACT
GCTTCAATACTGGCAAGTGTGCCATTAATTGTTATTTTTATTTTCATGATGATATCATTT
TTAATCATTTTAGGGCGCGATAGAATTAAACTTGAAACGCGTGCTGAAAAATTAAAAGAA
GTTGAACGTCGTTCATTGCGAATCGTTCAAGTATCAGAAGAAGAACAAGACGATAATTTA
TAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_2549 [new locus tag: SAUSA300_RS14155 ]
- symbol: BccT
- description: BCCT family choline/carnitine/betaine transporter
- length: 540
- theoretical pI: 9.09649
- theoretical MW: 60254
- GRAVY: 0.619259
⊟Function[edit | edit source]
- TIGRFAM: Transport and binding proteins Other transporter, betaine/carnitine/choline transporter (BCCT) family (TIGR00842; HMM-score: 460.9)
- TheSEED :
- Betaine/carnitine/choline transporter (BCCT) family
- PFAM: APC (CL0062) BCCT; BCCT, betaine/carnitine/choline family transporter (PF02028; HMM-score: 502.5)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 12
- LocateP: Multi-transmembrane
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0.17
- Signal peptide possibility: 0.5
- N-terminally Anchored Score: -1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.242236
- TAT(Tat/SPI): 0.005112
- LIPO(Sec/SPII): 0.056956
- predicted transmembrane helices (TMHMM): 12
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MKKEKVMDWTTFIGTVAVLLFAVIPMMAFPKASEDIITGINSAISDSIGSIYLFMGLAIFCFVMYIAFGKYGNVTLGKASDKPEFNTFTWAAMLFCAGIGSDILYWGVIEWAFYYQVPPNGAKSMSDEALQYATQYGMFHWGPIAWAIYVLPALPIGYLVFVKKQPVYKISQACRPILKGQTDKFVGKVVDILFIFGLLGGAATSLALGVPLISAGIERLTGLDGKNMILRSAILLTITVIFAISSYTGLKKGIQKLSDINVWLSFVLLAFIFIIGPTVFIMETTVTGFGNMLRDFFHMATWLEPFGGIKGRKETNFPQDWTIFYWSWWLVYAPFIGLFIARISKGRRLKEVVLGTIIYGTLGCVLFFGIFGNYAVYLQISGQFNVTQYLNTHGTEATIIEVVHHLPFPSLMIVLFLVSAFLFLATTFDSGSYILAAASQKKVVGEPLRANRLFWAFALCLLPFSLMLVGGERALEVLKTASILASVPLIVIFIFMMISFLIILGRDRIKLETRAEKLKEVERRSLRIVQVSEEEQDDNL
⊟Experimental data[edit | edit source]
- experimentally validated:
- protein localization: data available for COL
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator: ArgR (repression) regulon
ArgR (TF) important in Arginine biosynthesis, Arginine degradation; RegPrecise
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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